Mouse Pro-opiomelanocortin (POMC) ELISA KIT

Instructions
Code CSB-EL018363MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Pro-opiomelanocortin (POMC)
Alternative Names Pomc ELISA Kit; Pomc1Pro-opiomelanocortin ELISA Kit; POMC ELISA Kit; Corticotropin-lipotropin) [Cleaved into: NPP; Melanotropin gamma ELISA Kit; Gamma-MSH); Corticotropin ELISA Kit; Adrenocorticotropic hormone ELISA Kit; ACTH); Melanocyte-stimulating hormone alpha ELISA Kit; Alpha-MSH ELISA Kit; Melanotropin alpha); Corticotropin-like intermediary peptide ELISA Kit; CLIP); Lipotropin beta ELISA Kit; Beta-LPH); Lipotropin gamma ELISA Kit; Gamma-LPH); Melanocyte-stimulating hormone beta ELISA Kit; Beta-MSH ELISA Kit; Melanotropin beta); Beta-endorphin; Met-enkephalin] ELISA Kit
Abbreviation POMC
Uniprot No. P01193
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.73 pg/mL-3000 pg/mL
Sensitivity 0.18 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse POMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-92  
1:2 Average % 100  
Range % 95-110  
1:4 Average % 90  
Range % 88-94  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of mouse POMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 98 93-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
3000 0.227 0.219 0.223    
750 0.304 0.298 0.301    
187.5 0.475 0.456 0.466    
46.88 0.752 0.705 0.729    
11.72 1.256 1.223 1.240    
2.93 1.573 1.614 1.594    
0.73 1.811 1.742 1.777    
0 2.268 2.297 2.283    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Corticotropin
Gene References into Functions
  1. Authors show that renal-denervated WT and diabetic mice recapitulate the phenotype of improved glucose tolerance and elevated glycosuria associated with reduced renal GLUT2 levels observed in obese ArcPomc(-/-) mice. PMID: 29031726
  2. Alpha - MSH enhanced leptin sensitivity and preadipocyte proliferation, meanwhile inhibited endoplasmic reticulum stress of preadipocytes by activating Notch1 signal. PMID: 27717825
  3. Glutamate transporter-associated protein 3-18 (GTRAP3-18), an anchor protein that retains interacting proteins in the endoplasmic reticulum, is a critical regulator of food intake and body weight by interacting with POMC. GTRAP3-18-deficient mice showed hypophagia, lean bodies, and lower blood glucose, insulin, and leptin levels with increased serum and brain alpha-MSH levels, leading to AMPK inhibition. PMID: 28904020
  4. Immunostaining of serial sections of the hypothalamus revealed that POMC-expressing neurons were smaller in WNK1 TG mice than in WT mice. PMID: 29392534
  5. Selenoprotein T is a novel oligosaccharyltransferase subunit that regulates unfolded protein response signaling and ACTH secretion. PMID: 28928140
  6. Study shows that alpha melanocyte stimulating hormone and forkhead box C2 protein promote fatty acid oxidation through C/EBPbeta negative transcription in mice adipose tissue. PMID: 27819350
  7. Our findings demonstrate alphaMSH plays a key role in the prevention of adipose inflammation and inflammatory diseases by down-regulating Akt/JNK signal pathway and negatively interacting with FoxOs, which brings up alphaMSH as a novel candidate factor in the adipose anti-inflammation process in obesity. PMID: 28514752
  8. Data indicate the regulation of energy balance in BMPR1A-mediated appetite regulation in POMC neurons. PMID: 29040444
  9. POMC and AgRP neurons receive direct steroid- and frequency-dependent glutamatergic synaptic input from Kiss1(ARC) neurons in male mice PMID: 27093227
  10. Localization of the dopamine receptors of types 1 and 2 on the bodies of POMC-expressing neurons of the arcuate nucleus of the hypothalamus in mice and rats.( PMID: 28429261
  11. These findings indicate that the relevance of AgRP to POMC neuron GABA connectivity depends on the state of AgRP neuron activity and suggest that different types of transmitter release should be considered when circuit mapping. PMID: 28667175
  12. To clarify the functional roles of the AKT-mTOR pathway in the hippocampal neurons, we generated conditional knockout mice harboring the deletion of Pten (Pten-cKO) in Proopiomelanocortin-expressing neurons. PMID: 26961412
  13. Epo regulates hypothalamus POMC expression via STAT3 activation. PMID: 26563310
  14. Data suggest that the solitary tract pro-opiomelanocortin (NTSPOMC) neurons were involved in cardiorespiratory control and endogenous analgesia. PMID: 27077912
  15. hypothalamic arcuate nucleus (Arc)POMC-deficient mice, which develop severe obesity and insulin resistance, unexpectedly exhibit improved glucose tolerance and remain protected from hyperglycemia via decreased renal GLUT2 expression and glycosuria. PMID: 26467632
  16. Hypomethylation of POMC promoter occurred in the hypothalamus of the high sucrose diet offspring compared with controls. PMID: 25936720
  17. Alpha-MSH may play an important role in the itching associated with pigmented cutaneous lesions and that the histamine released from keratinocytes is involved in this alpha-MSH-induced itching. PMID: 26358220
  18. These data indicate that glucose induces distinct excitatory synaptic plasticity in different subpopulations of POMC neurons. PMID: 25127258
  19. Although genetic deletion of either Atg12 or Atg5 renders POMC neurons autophagy-deficient, mice lacking Atg5 in POMC neurons do not exhibit these phenotypes. PMID: 25585051
  20. these results reveal that cooperative interactions between the enhancers confer robustness of Pomc expression against gene regulatory disturbances and preclude deleterious metabolic phenotypes caused by Pomc deficiency in adulthood PMID: 25671638
  21. In contrast to mice with total hypothalamic Pomc deficiency, which are severely obese, mice with Lepr-restricted Pomc expression displayed fully normal body weight, food consumption, glucose homeostasis, and locomotor activity. PMID: 25594696
  22. Data suggest FoxO1 (forkhead box O1) region Gly140-Leu160 (Gln145, Arg147, Lys148, Arg153, Arg154) is critical for FoxO1 interaction with Stat3 (signal transducer/activator of transcription 3) in down-regulation of leptin-induced Pomc transcription. PMID: 25510553
  23. increased plasma ACTH levels in chronic psychosocially stressed male mice PMID: 24376791
  24. Data (including data from knockout mice) suggest that mobilization of neutrophils during stress requires expression of annexin A1 and likely involves ACTH-mediated activation/up-regulation of various receptors on surface membrane of neutrophils. PMID: 25184992
  25. ACTH activates Brown adipose tissue and browning of white adipose tissue while corticosterone counteracts this PMID: 25085924
  26. we demonstrate that POMC gene delivery suppressed melanoma growth by inducing apoptosis, which was at least partly dependent on Nox4 upregulation. PMID: 24412703
  27. alpha-MSH overexpression in the nucleus tractus solitarius decreases fat mass and elevates heart rate. PMID: 24829220
  28. Data indicate that early-life exposure to ethanol disrupts circadian oscillations of gene expression of proopiomelanocortin (POMC) and metabolic genes in POMC neurons. PMID: 24797626
  29. Rab3a deficiency promotes constitutive secretion and underlies selective impairment of Ca(2)-dependent release of alpha-MSH PMID: 24205339
  30. Data suggest changes in DNA methylation regulate expression of pituitary Pomc; CpG site-specific methylation of Pomc promoter represses mRNA transcription; reduction in DNA methylation occurs with delay after early-life stress and persists 1 year. PMID: 24506071
  31. postnatal ablation of POMC neurons leads to enhanced anxiety and the development of obesity despite decreased food intake and glucocorticoid deficiency. PMID: 23676213
  32. Gamma-2MSH directly regulates both ENaCalpha expression and cellular localization in the inner medulla to exert its natriuretic effect. PMID: 23871693
  33. This study demonstrated that both glutamatergic and GABAergic cells comprise significant, distinct subpopulations of hypothalamic POMC neurons. PMID: 23640796
  34. Data indicate the cellular and molecular mechanisms that underlie proopiomelanocortin (POMC) neuronal function under normal and perturbed physiological conditions in immortalized neuronal cell lines. PMID: 24134870
  35. NF-kappaB suppresses food intake and energy expenditure in mice by directly activating the Pomc promoter PMID: 23370526
  36. alpha-MSH and NPY modulate phagocytic activity in macrophages. PMID: 23689030
  37. Our results show dynamic changes in POMC in hypothalamic and extra-hypothalamic regions that may contribute to the negative affective/emotional state of heroin withdrawal PMID: 23337531
  38. The authors show a negative correlation between miR-375 and Pomc expression and suggest that miR-375 is a novel negative regulator of POMC expression and related hormone secretion. PMID: 23430746
  39. Additional studies demonstrate that arcuate nucleus pro-opiomelanocortin (POMC) expressing neurons are unresponsive to leptin PMID: 23341784
  40. In females, adult exposure to the high fat diet increased POMC expression in the arcuate nucleus. There was no change for males. There were no effects of BPA or DES exposure. PMID: 23493373
  41. Cordycepin decreased the phosphorylation of CREB induced by alpha-MSH and IBMX in B16F10 melanoma cells. PMID: 21972008
  42. Pomc expression has an effect on diverse immature neuronal populations PMID: 22166984
  43. symptoms of atopic dermatitis such as scratching can be exacerbated by alpha-MSH, which is induced by iNOS-derived NO PMID: 21895774
  44. The coordinate expression of Etv1 with POMC cell differentiation and its interaction with the highly cell-restricted Tpit factor indicate that Etv1 participates in a combinatorial code for pituitary cell-specific gene expression. PMID: 21622576
  45. Apelin and the proopiomelanocortin system: a new regulatory pathway of hypothalamic alpha-MSH release. PMID: 21846903
  46. SGK1 is strongly stimulated by glucocorticoids in pituitary corticotrophs; however, its effects on POMC transcription are antagonistic to the classical inhibitory glucocorticoid action PMID: 21586695
  47. Pituitary expression of POMC gene relies on a upstream enhancer that complements the activity of the proximal promoter with Tpit as the major regulator of both regulatory regions. The enhancer is conserved among species but not in hormone responsiveness. PMID: 21193556
  48. These data show that PTP1B in proopiomelanocortin neurons plays a role in short-term cold-induced reduction of spontaneous physical activity PMID: 21406615
  49. Data show that the phenotype of a mouse lacking both POMC and AgRP (DKO) is indistinguishable from that of mice lacking Pomc alone. PMID: 21363936
  50. The results shed light on the sex dependent differences in brain functioning and the role of Sry in the beta endorphin system related to the higher frequency of autistic disorder in males. PMID: 21408198
  51. The design, generation, validation, and preliminary analysis of one such partial POMC mutant specifically lacking alpha-MSH, is reported. PMID: 21195130
  52. These studies confirm an essential involvement of POMC peptides and of adrenal glucocorticoids and catecholamines on glucose homeostasis critical for early postnatal survival. PMID: 21184805
  53. alpha-MSH(1-13) is an in vivo substrate of PREP. PMID: 19490636
  54. Data reveal beta-endorphin-containing T cells as a crucial component of beneficial adaptive immune responses associated with painful peripheral nerve injuries. PMID: 20385224
  55. beta-E moderates behavioral responses to stressful stimuli and may have a role in coping behavior.The effects of EtOH on the response to stress may be mediated by beta-E. PMID: 20384608
  56. decreased plasma IL-10 levels but increased IFN-gamma in the model of pollinosis PMID: 20299824
  57. opiomelanocortin peptide and its opioid cleavage product, beta-endorphin, is expressed in the mouse retina PMID: 20533364
  58. results further support the existence of POMC projections from the hypothalamus to the NTS and suggest that these projections have a functional role in the control of food intake PMID: 20071607
  59. PTP1B and SHP2 as important components of POMC neuron regulation of energy balance and point to what we believe to be a novel role for SHP2 in the normal function of the melanocortin system. PMID: 20160350
  60. The CaR couples to Galpha(s) and regulates PTHrP and ACTH secretion in pituitary cells. PMID: 20032198
  61. The overexpression of Asb-4 in POMC neurons increased POMC mRNA expression in the arcuate nucleus. PMID: 19934378
  62. The study reports the identification of a Dexras1 interactor, prenylated Rab acceptor domain family member 1, which plays a novel role in ACTH stimulated secretion. PMID: 19733236
  63. Beta-endorphin also serves as a growth factor that regulates preimplantation development. PMID: 19994794
  64. 7B2-related peptides modulate regulated secretion of ACTH in neuroendocrine cells. PMID: 11852092
  65. fenfluramine-induced anorexia requires activation of central nervous system melanocortin pathways PMID: 12142539
  66. proopiomelanocortin is sorted to the regulated secretory pathway at the trans-Golgi network by a mechanism involving the interaction of a specific sorting signal on these molecules with the sorting receptor [review] PMID: 12438160
  67. mRNA expression of Pomc was up-regulated in Prader-Willi syndrome neonatal mice. PMID: 12443978
  68. CRH upregulates POMC mRNA expression in mouse skin in vitro. Activation of central hypothalamo-pituitary-adrenal axis may over ride activation of cutaneous CRH-POMC mechanism in development of DNFB-stimulated allergic contact dermatitis. PMID: 12637208
  69. In normal, nonsensitized mice, a nasal antigen challenge results in enhanced alpha-MSH production in the lung; sensitized mice treated with alpha-MSH demonstrate a strong anti-inflammatory effect of alpha-MSH in airways and lung that is IL-10 dependent. PMID: 12817018
  70. Data suggest that POMC-derived beta-endorphin may function in at least two primary modes to modulate feeding. PMID: 12851316
  71. Mice deficient in proopiomelanocortin peptides were obese, their adrenals were atrophied, they had undetectable plasma corticosterone in basal and stressed states, and they suffered perinatal lethality when intercrossed. PMID: 12851317
  72. Results substantiate a pivotal role of the pro-opiomelanocortin gene products in integrating metabolism. PMID: 12851327
  73. POMC mutants have normal levels of insulin, normal fasting glucose, and normal clearance of glucose thus insulin production, sensitivity and glucose uptake is functioning but homozygous POMC mutants do not recover from insulin-induced hypoglycemia. T PMID: 12970157
  74. POMC transgene attenuated fasting-induced hyperphagia in wild-type mice PMID: 14578285
  75. alpha-melanocyte-stimulating hormone has a role in collagen metabolism PMID: 14645373
  76. regulation of corticotroph NF-kappaB activity by corticotropin releasing hormone is related to the activation of the pituitary proopiomelanocortin gene PMID: 14711817
  77. Nur77 inuction and activation are necessary for IL-1 stimulation of POMC in AtT-20 corticotrophs. PMID: 15063754
  78. Pomc gene is sufficient to render mice susceptible to the effects of high fat feeding emphasizes the potential importance of this locus as a site for gene-environment interactions predisposing to obesity, as seen in knockout mice. PMID: 15070780
  79. Murine adrenal glands can develop without exposure to endogenous POMC-derived peptides during fetal and neonatal life. Such glands are atrophic and hypofunctional. Exposure to ACTH alone can restore their size, morphology, and corticosterone secretion. PMID: 15231703
  80. melanocortin peptides and the opioid beta-endorphin processed from POMC mediate the homeostatic and behavioral responses linked to POMC neuronal circuits PMID: 15481808
  81. proopiomelanocortin is not required for eumelanogenesis in mouse hair PMID: 15564334
  82. In addition to its protective effect on endocrine cells, ACTH controls adult adrenal cortex trophicity through an additional paracrine mechanism implying maintenance of the vasculature by VEGF. PMID: 15666790
  83. Rb and p107 are present on the POMC promoter and inhibition of their expression decreases POMC mRNA levels PMID: 15701640
  84. results suggest that cyclic-ADP-ribose is a second messenger in pituitary cells and regulates ACTH secretion by a mechanism dependent on activation of the ryanodine channel by extracellular Ca2+ PMID: 15718277
  85. POMC peptides are not required for prenatal adrenal development and that POMC peptides in addition to ACTH are required for postnatal proliferation and maintenance of adrenal structures capable of producing both glucocorticoids and mineralocorticoids. PMID: 15731356
  86. Two phylogenetically conserved neuronal POMC enhancers designated nPE1 (600 bp) and nPE2 (150 bp) located approximately 10 to 12 kb upstream of POMC transcriptional units were identified. PMID: 15798195
  87. Data demonstrate that CNS proopiomelanocortin peptides play a critical role in energy homeostasis that is not substituted by peripheral proopiomelanocortin . PMID: 16440060
  88. ACTH affects the expression profile of Y1 adrenal cells principally through cAMP- and protein kinase A- dependent signaling PMID: 16484322
  89. Both POMC null homozygous and heterozygous mutants also develop pituitary gland tumors, which result in their premature death. PMID: 16914086
  90. requirement for Stat3 in transcriptional regulation of Pomc but this circuit is only one of several components that underlie the neuronal response to leptin PMID: 17023536
  91. Neuronal POMC peptides are necessary to regulate corticotrophin-releasing hormons(CRH). Chronic reduction or absence of hypothalamic POMC leads to trophic stimulation of pituitary cells directly or indirectly through elevated CRH. PMID: 17095588
  92. Hypo- and hyperglycemia are detected by distinct populations of glucosensing neurons, and POMC and neuropeptide Y neurons are not solely responsible for arcuate nucleu glucose sensing. PMID: 17261674
  93. Long-term melanocortin activation reduces body weight, adiposity, and hepatic fat accumulation and improves glucose metabolism, particularly in the setting of diet-induced obesity. PMID: 17374695
  94. analysis of pituitary POMC conserved transcriptional regulation in vertebrates despite great promoter sequence divergence PMID: 17698954
  95. Pomc genotype was a strong predictor of dietary fat preference with Pomc null animals choosing to eat approximately twice as much fat, but similar amounts of carbohydrate and protein, as wild-type animals. PMID: 17717049
  96. The mechanism for obesity-induced loss of glucose sensing in POMC neurons involves uncoupling protein 2 (UCP2), a mitochondrial protein that impairs glucose-stimulated ATP production PMID: 17728716
  97. Agouti-related peptide induced delayed and long-lasting modifications of energy balance in Pomc-deficient but not glucocorticoid-deficient Pomc PMID: 17909095
  98. aldosterone production and release in vivo do not require the action of ACTH during development or postnatal life and that secondary hyperaldosteronism in Pomc(-/-) mice is a consequence of glucocorticoid deficiency PMID: 17991729
  99. AgRP/NPY and POMC neurons each play mandatory roles in aspects of leptin-regulated energy homeostasis. PMID: 18162515
  100. The direct stimulatory effect of lipopolysaccharides on POMC gene expression via TLR (probably TLR4) is presented, although the intracellular signaling pathways in the corticotroph may be different from those in immune cells. PMID: 18323674

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Subcellular Location Secreted
Protein Families POMC family
Tissue Specificity ACTH and MSH are produced by the pituitary gland.
Database Links

KEGG: mmu:18976

STRING: 10090.ENSMUSP00000020990

UniGene: Mm.277996

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