Mouse Pro-opiomelanocortin (POMC) ELISA KIT

Code CSB-EL018363MO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01193
Lead Time 3-5 working days
Abbreviation POMC
Target Name Pro-opiomelanocortin (POMC)
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.73 pg/mL-3000 pg/mL
Sensitivity 0.18 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse POMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-92  
1:2 Average % 100  
Range % 95-110  
1:4 Average % 90  
Range % 88-94  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of mouse POMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 98 93-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
3000 0.227 0.219 0.223    
750 0.304 0.298 0.301    
187.5 0.475 0.456 0.466    
46.88 0.752 0.705 0.729    
11.72 1.256 1.223 1.240    
2.93 1.573 1.614 1.594    
0.73 1.811 1.742 1.777    
0 2.268 2.297 2.283    
References
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Function Corticotropin
Subcellular Location Secreted
Protein Families POMC family
Tissue Specificity ACTH and MSH are produced by the pituitary gland.
Database Links

KEGG: mmu:18976

STRING: 10090.ENSMUSP00000020990

UniGene: Mm.277996

Pathway Melanogenesis
Adipocytokine signaling pathway

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