Mouse Protein FAM132B(FAM132B) ELISA kit

Instructions
Code CSB-EL008059MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name family with sequence similarity 132, member B
Alternative Names Erfe ELISA Kit; C1qtnf15 ELISA Kit; Ctrp15 ELISA Kit; Fam132bErythroferrone ELISA Kit; Complement C1q tumor necrosis factor-related protein 15 ELISA Kit; Myonectin ELISA Kit
Abbreviation FAM132B
Uniprot No. Q6PGN1
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.68 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FAM132B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %85
Range %80-90
1:2Average %93
Range %87-99
1:4Average %102
Range %98-107
1:8Average %98
Range %94-102
Recovery
The recovery of mouse FAM132B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-101
EDTA plasma (n=4)8984-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12002.388 2.533 2.461 2.297
6001.560 1.588 1.574 1.410
3000.978 0.901 0.940 0.776
1500.622 0.602 0.612 0.448
750.363 0.355 0.359 0.195
37.50.274 0.295 0.285 0.121
18.750.189 0.202 0.196 0.032
00.159 0.168 0.164
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage
Gene References into Functions
  1. Erfe(-/-) mice failed to adequately suppress hepcidin expression after infection with Plasmodium berghei K173. Importantly, the sustained production of hepcidin allowed by erythroferrone ablation was associated with decreased parasitemia, providing further evidence that transient iron restriction could be beneficial in the treatment of malaria. PMID: 27658439
  2. The here presented data unearthed EPO-dependent erythroferrone expression acts as an erythropoiesis-driven regulator of iron metabolism under phenylhydrazine-induced hemolytic anemia. PMID: 27067488
  3. Erythroferrone contributes to hepcidin suppression and iron overload in a mouse model of beta-thalassemia. PMID: 26276665
  4. Compared with wild-type mice, Fam132b-deficient mice exhibited a more severe anemia, had higher hepcidin levels and consequently lower serum iron concentration on days 14 and 21, and manifested impaired mobilization of iron from stores (liver and spleen). PMID: 25193872
  5. myonectin is a novel regulator of cellular autophagy PMID: 24187137

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Subcellular Location Secreted
Protein Families Adipolin/erythroferrone family
Tissue Specificity Predominantly expressed in skeletal muscle and, at much lower levels, in other tissues, including lung, eye, smooth muscle, heart, brain and kidney. Within skeletal muscles, higher expression levels in soleus as compared with plantaris. Found in blood (at
Database Links

KEGG: mmu:227358

STRING: 10090.ENSMUSP00000084073

UniGene: Mm.389157

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