Mouse Protein FAM132B(FAM132B) ELISA kit

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Target Background

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Product Details

Description

This Mouse FAM132B ELISA Kit was designed for the quantitative measurement of Mouse FAM132B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.

Target Name

family with sequence similarity 132, member B

Alternative Names

Erfe ELISA Kit; C1qtnf15 ELISA Kit; Ctrp15 ELISA Kit; Fam132bErythroferrone ELISA Kit; Complement C1q tumor necrosis factor-related protein 15 ELISA Kit; Myonectin ELISA Kit

Abbreviation

FAM132B

Uniprot No.

Q6PGN1

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

18.75 pg/mL-1200 pg/mL

Sensitivity

4.68 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FAM132B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	93
	Range %	87-99
1:4	Average %	102
	Range %	98-107
1:8	Average %	98
	Range %	94-102

Recovery

The recovery of mouse FAM132B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	89	84-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.388	2.533	2.461	2.297
600	1.560	1.588	1.574	1.410
300	0.978	0.901	0.940	0.776
150	0.622	0.602	0.612	0.448
75	0.363	0.355	0.359	0.195
37.5	0.274	0.295	0.285	0.121
18.75	0.189	0.202	0.196	0.032
0	0.159	0.168	0.164

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Related Products

Target Background

Function (From Uniprot)	Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.
Gene References into Functions	Erfe(-/-) mice failed to adequately suppress hepcidin expression after infection with Plasmodium berghei K173. Importantly, the sustained production of hepcidin allowed by erythroferrone ablation was associated with decreased parasitemia, providing further evidence that transient iron restriction could be beneficial in the treatment of malaria. PMID: 27658439 The here presented data unearthed EPO-dependent erythroferrone expression acts as an erythropoiesis-driven regulator of iron metabolism under phenylhydrazine-induced hemolytic anemia. PMID: 27067488 Erythroferrone contributes to hepcidin suppression and iron overload in a mouse model of beta-thalassemia. PMID: 26276665 Compared with wild-type mice, Fam132b-deficient mice exhibited a more severe anemia, had higher hepcidin levels and consequently lower serum iron concentration on days 14 and 21, and manifested impaired mobilization of iron from stores (liver and spleen). PMID: 25193872 myonectin is a novel regulator of cellular autophagy PMID: 24187137 Show More Hide All
Subcellular Location	Secreted.
Protein Families	Adipolin/erythroferrone family
Tissue Specificity	Predominantly expressed in skeletal muscle and, at much lower levels, in other tissues, including lung, eye, smooth muscle, heart, brain and kidney. Within skeletal muscles, higher expression levels in soleus as compared with plantaris. Found in blood (at
Database Links	KEGG: mmu:227358 STRING: 10090.ENSMUSP00000084073 UniGene: Mm.389157

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Check Code：

Code	CSB-EL008059MO
Size	96T,5×96T,10×96T
Trial Size
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.