Recombinant Mouse Erythroferrone (Erfe)

Code CSB-BP757647MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-EP757647MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-MP757647MO
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Erfe
Uniprot No.
Alternative Names
Erfe; C1qtnf15; Ctrp15; Fam132bErythroferrone; Complement C1q tumor necrosis factor-related protein 15; Myonectin
Species
Mus musculus (Mouse)
Expression Region
25-340
Target Protein Sequence
LGVPES AEPVGTHARP QPPGAELPAP PANSPPEPTI AHAHSVDPRD AWMLFVKQSD KGINSKRRSK ARRLKLGLPG PPGPPGPQGP PGPFIPSEVL LKEFQLLLKG AVRQRESHLE HCTRDLTTPA SGSPSRVPAA QELDSQDPGA LLALLAATLA QGPRAPRVEA AFHCRLRRDV QVDRRALHEL GIYYLPEVEG AFHRGPGLNL TSGQYTAPVA GFYALAATLH VALTEQPRKG PTRPRDRLRL LICIQSLCQH NASLETVMGL ENSSELFTIS VNGVLYLQAG HYTSVFLDNA SGSSLTVRSG SHFSAILLGL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.
Gene References into Functions
  1. Erfe(-/-) mice failed to adequately suppress hepcidin expression after infection with Plasmodium berghei K173. Importantly, the sustained production of hepcidin allowed by erythroferrone ablation was associated with decreased parasitemia, providing further evidence that transient iron restriction could be beneficial in the treatment of malaria. PMID: 27658439
  2. The here presented data unearthed EPO-dependent erythroferrone expression acts as an erythropoiesis-driven regulator of iron metabolism under phenylhydrazine-induced hemolytic anemia. PMID: 27067488
  3. Erythroferrone contributes to hepcidin suppression and iron overload in a mouse model of beta-thalassemia. PMID: 26276665
  4. Compared with wild-type mice, Fam132b-deficient mice exhibited a more severe anemia, had higher hepcidin levels and consequently lower serum iron concentration on days 14 and 21, and manifested impaired mobilization of iron from stores (liver and spleen). PMID: 25193872
  5. myonectin is a novel regulator of cellular autophagy PMID: 24187137

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Subcellular Location
Secreted.
Protein Families
Adipolin/erythroferrone family
Tissue Specificity
Predominantly expressed in skeletal muscle and, at much lower levels, in other tissues, including lung, eye, smooth muscle, heart, brain and kidney. Within skeletal muscles, higher expression levels in soleus as compared with plantaris. Found in blood (at
Database Links
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