Mouse Protein Wnt-3a(WNT3A) ELISA kit

Code CSB-EL026136MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
wingless-type MMTV integration site family, member 3A
Alternative Names
Wnt3a ELISA kit; Wnt-3aProtein Wnt-3a ELISA kit
Abbreviation
WNT3A
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Stem Cells
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %90-95
1:2Average %89
Range %85-94
1:4Average %94
Range %89-97
1:8Average %98
Range %95-100
Recovery
The recovery of mouse WNT3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-95
EDTA plasma (n=4)9591-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.886 2.864 2.875 2.778
7502.021 2.011 2.016 1.919
3751.152 1.132 1.142 1.045
187.50.645 0.638 0.642 0.545
940.364 0.378 0.371 0.274
470.239 0.255 0.247 0.150
23.50.162 0.172 0.167 0.070
00.095 0.098 0.097  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse WNT3A ELISA Kit was designed for the quantitative measurement of Mouse WNT3A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL .

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Target Background

Function
(From Uniprot)
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).
Gene References into Functions
  1. This work provides a new link between Wnt3a and macrophage-mediated angiogenesis under glucose and oxygen deprivation in vitro. PMID: 29199847
  2. Lrp6 is the key mediator of Wnt3a signaling in osteoblasts and Lrp5 played a less significant role in mediating Wnt3a signaling. PMID: 29176883
  3. These data define the mechanism responsible for the repressive effects of nitric oxide (NO) on the transcriptional activity of beta-catenin and link eNOS-derived NO to the modulation by VEGF of Wnt/beta-catenin-induced endothelial cell proliferation. PMID: 28320874
  4. These results suggest novel mechanisms for Wnt3a-induced osteoblast proliferation and cell survival via Npnt gene expression PMID: 28093227
  5. this study shows that the Wnt3a expression levels in dendritic cells influences the generation of memory T cells after 5 days in co-culture with naive T cells through activation of the Wnt canonical pathway PMID: 27544306
  6. Wnt3a induces Osx expression via p38 MAPK signaling in dental follicle cells. Wnt3a-induced Osx expression was inhibited in the presence of p38 mitogen-activated protein kinase (MAPK) inhibitors (SB203580 and SB202190) at gene and protein levels, as assessed by real-time PCR and immunocytohistochemistry, respectively. PMID: 27450807
  7. Western blot analysis demonstrated that following BMP2 and BMP7 cotransfection of MC3T3E1 cells, the protein expression levels of BMP2, BMP4, BMP6, BMP7, BMP9 and Wnt3a were increased compared with control cells PMID: 27633082
  8. Wnt3a promotes macrophage-mediated bacterial killing by elevating CRAMP and BD1 levels. PMID: 26846714
  9. Wnt3a acutely reduces nuclear acetyl-CoA, the necessary substrate for histone acetyltransferases, resulting in a global decrease in histone acetylation. PMID: 27129247
  10. Wnt3a rapidly activated the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathway and pharmacological stimulation of cAMP/PKA signaling suppressed osteoclast differentiation PMID: 26189772
  11. Mesenchymal Stem Cells Within Gelatin/CaSO4 Scaffolds Treated Ex Vivo with Low Doses of BMP-2 and Wnt3a Increase Bone Regeneration. PMID: 26414873
  12. Wnt3a increased the expression of NeuroD1 and Ins2 in the hypothalamus. PMID: 26956881
  13. that activation of the Wnt pathway made cells more readily tolerate ROS-caused mitochondria injury and cell apoptosis PMID: 26085341
  14. Pin1 is indispensable for Wnt3a-induced osteoblast differentiation. PMID: 26740630
  15. The mouse xenograft model showed that Wnt3a-overexpressing cells grew into larger tumor masses and formed more vasculogenic mimicry than the control cells. PMID: 26266404
  16. miR-27-3p inhibits melanogenesis by repressing Wnt3a protein expression. PMID: 26006230
  17. Wnt8a and Wnt3a cooperate to maintain Fgf8 expression and prevent premature Sox2 up-regulation in the axial stem cell niche, critical for posterior growth. PMID: 25809880
  18. WNT3A promotes myogenesis of human embryonic stem cells and enhances in vivo heterologous engraftment. PMID: 25084050
  19. C1q-induced activation of Wnt3a/CTNNB1/FOXO1 signaling contributes to skeletal fiber type shift toward fatigable fiber in congestive heart failure. PMID: 26038536
  20. Study reports that NRF2 participates in the formation of a protein complex with Axin1/GSK-3/beta-TrCP that is disrupted by WNT-3A, a prototypic canonical WNT ligand, and leads to upregulation of the NRF2 transcriptional signature. PMID: 25336178
  21. Wnt3a and MMP9 induce C3H10T1/2 cells differentiation into cardiomyocyte-like cells. PMID: 25854560
  22. Wnt3a/beta-catenin signaling directs trunk progenitors towards paraxial mesodermal progenitor fates; however, our data also suggest that Wnt3a positively supports a progenitor state for both mesodermal and neural progenitors. PMID: 25922526
  23. the presented systems-biology approach demonstrates that Wnt3a attenuates RANKL-driven osteoclastogenesis by blocking c-Fos expression PMID: 25038457
  24. Wnt3a/Frizzled-3 signaling plays important role in regulating the proliferation and differentiation of neural crest cells and various developmental stages of melanocyte precursors. PMID: 24815018
  25. Sp5 and Sp8 are pivotal downstream effectors of Wnt3a/beta-catenin that are essential for the self-renewal and differentiation of neuromesodermal stem cells. PMID: 24475213
  26. results suggest that Wnt3a overrides the effect of P2X7R on the Wnt/beta-catenin signaling to prevent the AEC I death and restrict the severity of ALI. PMID: 24922070
  27. these data identify HES5 as a key mediator of the Wnt-3a proneurogenic effect occurring independently of the classical Wnt/beta-catenin signaling cascade thus further deciphering crosstalk mechanisms of Wnt and Notch signaling pathways regulating cell fate PMID: 24548083
  28. These results indicate that JBP regulates the expressions of SHP-1, Wnt3a, and AP-1 proteins in chemically damaged mice. PMID: 24841652
  29. our results suggest a dose-dependent role for Wnt3a in regulating the balance between renewal and selection of differentiation fates of axial progenitors in the epiblast. PMID: 25152336
  30. Mutation of any individual cysteine of Wnt3a results in covalent Wnt oligomers through ectopic intermolecular disulfide bond formation and diminishes/abolishes Wnt signaling. PMID: 24841207
  31. epigenetic activation of Bmp2 gene expression contributes to Wnt3a-mediated direct trans-differentiation of pre-adipocytes or fibroblasts into osteoblasts PMID: 24867947
  32. Increasing amounts of soluble Frizzled8-cysteine-rich-domain protein modulated Wnt3a signaling in a biphasic manner. PMID: 24885675
  33. results demonstrate that, while ER signaling acts synergistically with Wnt3A in promoting osteogenic differentiation, Wnt3A may crosstalk with ER signaling by up-regulating ERalpha expression and down-regulating ERbeta expression in MPCs. PMID: 24340027
  34. Wnt3a/beta-catenin signaling regulates follicular development by modulating the expression of Foxo3a signaling components. PMID: 24246780
  35. Smad4 regulates maturation of skeletal collagen and osteoblast survival, and is required for matrix-forming responses to both BMP2 and Wnt3a. PMID: 24006258
  36. Jianpi Bushen Prescription plays a protective role on radiation-induced bone marrow through the activation of the Wnt3a signaling pathway. PMID: 24114209
  37. Structure-based mutational analysis of mouse Wnt3a shows that the linker between the N- and C-terminal domains is required for LRP6 binding. PMID: 23791946
  38. Results demonstrated that Wnt3a regulates proliferation, apoptosis and enhances function of pancreatic NIT-1 beta cells via activation of Wnt/beta-catenin signaling. PMID: 23296977
  39. Data indicate that Wnt/beta-catenin signaling mediates nandrolone-induced Numb transcription. PMID: 23649620
  40. WNTs have a dual and context-dependent effect on microglia acting in a homeostatic pro- and anti-inflammatory manner. PMID: 23534675
  41. We identified a number of stimuli triggering Wnt/beta-catenin pathway activation, including exogenous NKT cell activator, glycolipid alpha-GalCer, and endogenous prostaglandin E2 (PGE2). PMID: 22991247
  42. Results support a potential mechanism for the differentiation of mesenchymal stem cells into type II alveolar cells involving canonical wnt pathway activation. PMID: 23154940
  43. Wnt3a is a putative molecular partner of Zbtb20 in translating early positional information for the establishment of all developing medial pallidum fields. PMID: 22689450
  44. findings suggest that a spatially restricted Wnt signal induces an oriented cell division in embryonic stem cells that generates distinct cell fates at predictable positions relative to the Wnt source PMID: 23520113
  45. Wnt3a stimulates repaid translocation of IPMK to the cell membrane. PMID: 22940627
  46. results identify LRP8 as a novel positive factor of canonical Wnt signaling pathway and show its involvement in Wnt-induced osteoblast differentiation PMID: 22589174
  47. results of this study suggest that Wnt3a selectively stimulates proliferation of progenitors that are committed to differentiation or are in the process of exiting the SSC state, leading to enhanced formation of germ cell communities PMID: 22761943
  48. Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2. PMID: 22357953
  49. Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells. Wnt3a plays an important role in melanocyte homeostasis. PMID: 22710324
  50. These data suggest that stabilization of beta-catenin by WNT3A inhibits BMP2-mediated induction of cementoblast/osteoblast differentiation in SVF4 cells, although BMP2 requires endogenous Wnt/beta-catenin signaling to promote cell maturation. PMID: 22150562

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Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families
Wnt family
Tissue Specificity
Dorsal portion of the neural tube (developing roof plate), and mesenchyme tissue surrounding the umbilical veins.
Database Links
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