Mouse Protein Wnt-3a(WNT3A) ELISA kit

Code CSB-EL026136MO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P27467
Lead Time 5-7 working days
Abbreviation WNT3A
Protein Biological Process 1 Signaling Pathway
Target Name wingless-type MMTV integration site family, member 3A
Alias MGC119418, MGC119419, MGC119420,
Species Mus musculus (Mouse)
Protein Biological Process 3 Wnt signaling pathway
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region.
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %92
Range %90-95
1:2Average %89
Range %85-94
1:4Average %94
Range %89-97
1:8Average %98
Range %95-100
The recovery of mouse WNT3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-95
EDTA plasma (n=4)9591-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
15002.886 2.864 2.875 2.778
7502.021 2.011 2.016 1.919
3751.152 1.132 1.142 1.045
187.50.645 0.638 0.642 0.545
940.364 0.378 0.371 0.274
470.239 0.255 0.247 0.150
23.50.162 0.172 0.167 0.070
00.095 0.098 0.097  
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Function Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Wnt family
Tissue Specificity Dorsal portion of the neural tube (developing roof plate), and mesenchyme tissue surrounding the umbilical veins.
Database Links

KEGG: mmu:22416

STRING: 10090.ENSMUSP00000010044

UniGene: Mm.1367

Pathway Hippo signaling pathway
mTOR signaling pathway
Wnt signaling pathway
Signaling pathways regulating pluripotency of stem cells

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