Mouse Protein delta homolog 1(DLK1) ELISA kit

Instructions
Code CSB-EL006945MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name delta-like 1 homolog (Drosophila)
Alternative Names Dlk1 ELISA kit; Dlk ELISA kit; Pref1 ELISA kit; Scp-1Protein delta homolog 1 ELISA kit; DLK-1 ELISA kit; Adipocyte differentiation inhibitor protein ELISA kit; Preadipocyte factor 1 ELISA kit; Pref-1) [Cleaved into: Fetal antigen 1 ELISA kit; FA1)] ELISA kit
Abbreviation DLK1
Uniprot No. Q09163
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse DLK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %84-96
1:2Average %95
Range %91-99
1:4Average %94
Range %90-98
1:8Average %102
Range %92-108
Recovery
The recovery of mouse DLK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-102
EDTA plasma (n=4)8982-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.045 1.944 1.995 1.844
5001.644 1.523 1.584 1.433
2501.073 1.052 1.063 0.912
1250.673 0.662 0.668 0.517
62.50.371 0.382 0.377 0.226
31.20.284 0.273 0.279 0.128
15.60.235 0.231 0.233 0.082
00.148 0.153 0.151  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.
Gene References into Functions
  1. Functional analysis revealed that ground-state miRNAs embedded in the Dlk1-Dio3 locus (miR-541-5p, miR-410-3p, and miR-381-3p) promoted pluripotency via inhibition of multi-lineage differentiation and stimulation of self-renewal PMID: 29129685
  2. This study suggests that Pref-1 is an endocrine factor which is synergistically increased by obesity and age. PMID: 29138005
  3. Gene body methylation of noncoding RNA genes was observed and among these microRNA genes were prominent. Of particular note, observed only in hyperphenylalaninemic animals, was hypomethylation of miRNA genes within the imprinted Dlk1-Dio3 locus on chromosome 12. PMID: 26822703
  4. The role of elevated oxygen levels in eroding imprinted Dlk1 expression during prolonged culture and in vitro differentiation. PMID: 27729406
  5. Loss of DLK1 expression is associated with fetal growth retardation complications of pregnancy. PMID: 27776119
  6. Pref-1 mRNA is a novel substrate of RNase-L. PMID: 27831565
  7. the conserved sequences in IG-DMR are involved in the expression regulation of some of the imprinted genes in the Dlk1-Dio3 domain PMID: 27904015
  8. To investigate the nature of these more variably methylated secondary differentially methylated regions, we adopted a hairpin linker bisulfite mutagenesis approach to examine CpG dyad methylation at differentially methylated regions associated with the murine Dlk1/Gtl2 imprinting cluster on both complementary strands. PMID: 28649282
  9. NOTCH1 ligand Delta-like 1 homolog (DLK1) self interacts PMID: 28099888
  10. the salivary gland phenotype of Dlk1 knock-out mice, was investigated. PMID: 26711912
  11. The brain-specific miR-379/miR-410 gene cluster at the imprinted Dlk1-Dio3 domain is implicated in several aspects of brain development and function. PMID: 26744330
  12. data provide the first evidence for a direct interaction between DLK1 and NOTCH1 in mammals, and substantiate that non-canonical NOTCH ligands exist, adding to the complexity of NOTCH signaling. PMID: 26791579
  13. the Dlk1-Gtl2 locus plays a critical role in preserving long-term repopulating HSCs (LT-HSCs). PMID: 26627594
  14. DNA methylation regulates genomic imprinted DLK1-Dio3 miRNAs in autoimmune lupus PMID: 27070142
  15. identify the molecular regulators involved in the recruitment of AFF3 to gDMRs and provide mechanistic insight into the requirement of AFF3 at an enhancer for the expression of an approximately 200-kb polycistronic transcript within the imprinted Dlk1-Dio3 locus PMID: 26728555
  16. Dppa3 has a critical role in generation of fully reprogrammed iPS cells and maintenance of Dlk1-Dio3 imprinting PMID: 25613421
  17. results suggest that maternal expression of the imprinted miR-379/miR-544 cluster regulates paternal expression of the Dlk1 gene in mice PMID: 26487685
  18. Dll1 and Jag1, Notch ligands, function redundantly and are necessary to maintain the centroacinar cells as an environmental niche in the developing pancreas. PMID: 25919081
  19. Gtl2((-/+)) teratomas have decreased expression of 28 miRNAs encoded by the Dlk1-Dio3 domain PMID: 25355544
  20. DLK1 is a novel inflammatory inhibitor which interferes with NOTCH1 signaling in TLR-activated murine macrophages. PMID: 26115479
  21. these data suggest that miR-143 promotes adipogenesis by directly modulating the pref-1 expression in adipocytes. PMID: 25366176
  22. This study provides the spatiotemporal expression and dynamic changes of lncRNAs from Dlk1-Dio3 imprinted region in mouse preimplantation stage embryos and offers insight into the potential mechanisms responsible for Gtl2 activation. PMID: 26005002
  23. Pref-1 expressing cells are required for adipose tissue development. PMID: 25088414
  24. Data indicate that systemic delta-like 1 protein Dll1/delta-like4 protein Dll4 inhibition decreased T cell cytokines and graft infiltration. PMID: 25687759
  25. the function of DLK1 is to shift the metabolic mode of the organism toward peripheral lipid oxidation and away from lipid storage PMID: 25349437
  26. DNA methylation dynamics of a maternally methylated DMR in the mouse Dlk1-Dio3 domain PMID: 25447521
  27. Multiple Dlk1 splice variants are expressed during early mouse embryogenesis. PMID: 24860997
  28. DLK1 regulates submandibular salivary glands morphogenesis and parasympathetic nerve fibre outgrowth through inhibition of NOTCH signalling PMID: 24828459
  29. Upregulation of Prdm14 results in a highly homogeneous population of authentic pluripotent colonies and prevents the abnormal silencing of the Dlk1-Dio3 locus. PMID: 24552707
  30. Study characterized in detail AK044800, a transcript from the Dlk1-Dio3 imprinted region. PMID: 23515577
  31. Dlk1 isoforms have distinctive effects on myogenesis, and its regulation during myogenesis is critical for normal muscle development. PMID: 24582655
  32. Dlk1 is both necessary and sufficient for determining fast motor neurons and their corresponding biophysical signature in the mouse and chick. PMID: 24626931
  33. Dlk1 is a negative regulator of emerging hematopoietic stem and progenitor cells. PMID: 22801971
  34. delta-like 1 homolog (Dlk1), an imprinted gene best known for its ability to inhibit adipogenesis, is a crucial regulator of the myogenic program in skeletal muscle. PMID: 23946446
  35. data suggest that loss of Dlk1 gives rise to minor pituitary defects manifesting as an age- and sex-dependent reduction in pituitary hormone contents PMID: 23279263
  36. data identify Dlk1-Dio3 ncRNAs as novel candidate early biomarkers for mouse liver tumor promotion and provide new opportunities for assessing the carcinogenic potential of novel compounds PMID: 23091169
  37. Dlk1 expression is not essential for the normal development of beta-cells, somatotrophs and endothelial cells. PMID: 23059197
  38. Data indicate a set of 29 up-regulated miRNAs originating from the Dlk1-Dio3 genomic imprinted region, which has been identified as a hallmark of pluripotency and proliferation. PMID: 23554452
  39. Data indicate that DLK+ fetal hepatic progenitors cells support long-term expansion of hematopoietic stem cells (HSCs). PMID: 23415675
  40. Data also found that Dlk interrupts the binding between Cfr and Fgf18. The Fgf18 signaling pathway seems to be finely tuned by Cfr and Dlk for skeletal development. PMID: 20023171
  41. Data found that upon liver injury, Dlk1 was dramatically induced and initially expressed in hepatocytes and then into the HSCs by a paracrine manner. PMID: 21239501
  42. Data suggest that only membrane-bound Dlk1 (Dlk1-M) represses preadipocyte proliferation, not soluble form (Dlk1-S). Adult Dlk1-null mice exhibit higher amounts of fat than wildtype control mice, possibly due to increased preadipocyte replication. PMID: 22891218
  43. localize the majority of Dlk1 cis-regulatory elements to a 41 kb region upstream of the gene PMID: 22606264
  44. DLK1 may have a dual role in the hypothalamus: it may play a role in the post-natal hypothalamic maturation of the arginine vasopressin and oxytocin neurons. PMID: 22563444
  45. the imprinted delta-like homolog 1/preadipocyte factor (Dlk1/Pref1) and iodothyronine deiodinase type 3 (Dio3) functions converge on the development of brown fat at the transition to independent life. PMID: 22326222
  46. crosstalk between TGF-beta1 and Dlk1 was shown to promote early chondrogenesis during the embryonic endochondral ossification process. PMID: 22102178
  47. Dlk1-null mice showed increased levels of NOTCH downstream targets, such as Snail and Slug PMID: 22068159
  48. novel roles of HSC-derived DLK1 in activating HSCs via epigenetic Ppargamma repression and participating in liver regeneration and development in a manner involving the mesenchymal-epithelial interaction. PMID: 22298767
  49. These findings provide evidence supporting several regulatory functions of delta-like protein 1 (DLK1) in the pituitary gland. PMID: 21756269
  50. Dlk1/FA1 acts as an inhibitor of the PI3K/Akt pathways that leads to its inhibitory effects on chondrogenesis. PMID: 21724852
  51. genes in the Dlk1-Dio3 cluster play important roles in cell proliferation and/or differentiation, not only at late-gestation stages, but at developmental stages earlier than E11.5 PMID: 21620950
  52. Dlk1 is a novel regulator of bone mass that functions to inhibit bone formation and to stimulate bone resorption. PMID: 21308776
  53. mice that are deficient in Dlk1 have defects in postnatal neurogenesis in the subventricular zone: a developmental continuum that results in depletion of mature neurons in the olfactory bulb PMID: 21776083
  54. Data show that DLK1 and DLK2 act as inhibitory non-canonical protein ligands for the NOTCH1 receptor that modulate NOTCH signaling. PMID: 21419176
  55. Results suggest a model in which Dlk1 expressed by nascent or regenerating myofibers non-cell autonomously promotes the differentiation of their neighbor satellite cells and therefore leads to muscle hypertrophy. PMID: 21124733
  56. Meg3/Gtl2 and Dlk1/Peg9 genes are imprinted, with reciprocal expression from the maternal and paternal alleles, respectively. PMID: 10996796
  57. Dlk1 gene, like the human ortholog, is imprinted, with preferential expression from the paternal allele. PMID: 11168589
  58. activation of the imprinted Dlk1-Dio3 region correlates with pluripotency levels of mouse stem cells PMID: 20382743
  59. by interacting with fibronectin, Pref-1 activates integrin downstream signaling to activate MEK/ERK and to inhibit adipocyte differentiation PMID: 20457810
  60. miR-15a modulates DLK1 levels in preadipocytes thus providing a mechanism for DLK1 regulation that further links it to cell cycle arrest and cancer since miR-15a is deregulated in these processes PMID: 20385127
  61. Preadipocyte factor-1, a protein that suppresses differentiation and promotes colonocyte growth, may account in part for the sensitivity of A/J mice to azoxymeethane-induced carcinogenesis. PMID: 15297369
  62. Dlk1/FA1 is expressed specifically in cells undergoing transition from proliferating to prehypertrophic chondrocytes during endochondral ossification of the mouse limb. PMID: 20058200
  63. Data show that that lack of periostin in the embryonic OFT leads to ectopic expression of the proosteogenic growth factor pleiotrophin (Ptn) and overexpression of delta-like 1 homolog (Dlk1), a negative regulator of Notch1. PMID: 19723774
  64. Pref-1 has roles in adipogenesis and mesenchymal cell fate [review] PMID: 19541743
  65. Transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes. PMID: 19874716
  66. The level of expression of dlk is critical for 3T3-L1 fibroblasts to differentiate into adipocytes PMID: 11689349
  67. Dlk modulated signaling through the IGF-1 receptor,causing changes in ERK/MAPK levels that correlated with differentiation outcome. Dlk appears to modulate ERK/MAPK signaling in response to specific differentiation signals. PMID: 11900478
  68. Only the large soluble form of preadipocyte factor-1 (Pref-1), but not the small soluble and membrane forms, inhibits adipocyte differentiation: role of alternative splicing. PMID: 11988086
  69. Mice lacking paternally expressed Pref-1/Dlk1 display growth retardation, obesity, blepharophimosis, skeletal malformation, and increased serum lipid metabolites. Deficiency may produce most symptoms of maternal uniparental disomy syndrome PMID: 12101250
  70. efficient and regulated processing of Pref-1 occurs in 3T3-L1 preadipocytes releasing most of the extracellular domain as a 50-kDa heterogeneous protein, previously isolated and characterized as FA1. PMID: 12651852
  71. Pref-1 secretion controls fat cell differentiation and adipose tissue development. PMID: 12660879
  72. In preadipocytes Foxa-2 inhibits adipocyte differentiation by activating transcription of the Pref-1 gene. PMID: 12865419
  73. mechanism of imprinting at the Dlk1-Gtl2 domain PMID: 12937418
  74. dlk functionally interacts in vivo with Notch1, which may lead to the regulation of differentiation processes modulated by Notch1 activation and signaling PMID: 15652348
  75. Repression of Dlk1 requires HDAC3 deacetylase activity, which is recruited to the endogenous Dlk1 promoter where it interacts with KLF6. The interaction between HDAC3 and KLF6 is identified as a potential mechanism underlying adipogenesis. PMID: 15917248
  76. Dlk1 expression marks develoiping endothelium and sites of branching morphogenesis in the embryo and placenta. PMID: 16456855
  77. Identification of TACE as the major protease responsible for conversion of membrane-bound Pref-1 to the biologically active diffusible form provides a new insight into Pref-1 function in adipocyte differentiation. PMID: 16809777
  78. These data provide further evidence for the coregulation of the imprinted Dlk1 and Gtl2 genes, and support a role for Dlk1 as an important neonatal growth factor PMID: 17014736
  79. Mice lacking Pref-1 show accelerated fat deposition; conversely, mice overexpressing soluble Pref-1 in adipose tissue show a decrease in fat mass. PMID: 17116701
  80. interactions between DNA methylation and histone acetylation are involved in regulating the imprinting of the Dlk1-Gtl2 locus PMID: 17126526
  81. Preadipocyte factor 1 (Pref-1) activates MEK/ERK signaling, which is required for Pref-1 inhibition of adipogenesis[Preadipocyte factor 1] PMID: 17210639
  82. Adipogenesis may be modulated by the coordinated expression of Dlk1 and EGFL9/Dlk2. PMID: 17320102
  83. Dlk1 may potentiate or inhibit adipogenesis depending upon the cellular context, and that Notch1 expression and activation are important factors in this context. PMID: 17320900
  84. FA1 provides a novel class of developmental molecules that regulate physiological functions of the postnatal organisms. PMID: 17446189
  85. Dlk1 and the reciprocally imprinted non-coding RNA, Gtl2, have independent tissue-specific functions PMID: 17449025
  86. Data show that growth hormone mRNA, protein, and secretion were significantly decreased in GH3 pituitary cell clones that stably express Dlk1. PMID: 17485162
  87. inappropriate expression of Igf2 and Dlk1 is involved in impaired fetal hematopoiesis PMID: 18344616
  88. These data suggest that membrane dlk1 variants bind to extracellular IGFBP1/IGF-1 complexes, which may favor the release of IGF-1 and increase the local concentration of free IGF-1 that can enhance IGF receptor signaling, leading to adipogenesis. PMID: 18466921
  89. These results show that dlk expression is essential for normal B cell development. PMID: 18513163
  90. Ten imprinted genes were elucidated. The imprinting of three paternally expressed protein coding genes (Dlk1, Peg11, and Dio3) was confirmed. PMID: 19194500
  91. Embryos expressing a double dose of Dlk1 are growth enhanced but fail to thrive in early life. PMID: 19247431
  92. Results show that Sox9 downregulation is required for adipocyte differentiation and that Pref-1 inhibits adipocyte differentiation through upregulating Sox9 expression. PMID: 19254573
  93. PTTG1 and DLK1 are involved in cell differentiation and transformation. PMID: 19477929
  94. Dlk1 is required to prevent premature expression of Dat in meso-diencephalic dopamine neuronal precursors as part of the multifaceted process of neuronal differentiation driven by Nurr1 and Pitx3. PMID: 19515692
  95. expression of EpCAM and DLK1 suggests the developmental pathways of mouse liver progenitors PMID: 19527784
  96. Data suggest that DLK1(+) adipose stromal vascular fraction cells are of a vascular origin and not committed preadipocytes as assumed hitherto. PMID: 19665021
  97. Soluble Pref-1 activates MEK/ERK phosphorylation in a time and dose dependent manner to inhibit adipocyte differentiation. PMID: 17210639

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Subcellular Location Membrane, Single-pass type I membrane protein
Tissue Specificity Highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary and, to a lesser degree, in adult kidney, muscle, thymus and heart.
Database Links

KEGG: mmu:13386

STRING: 10090.ENSMUSP00000063104

UniGene: Mm.157069

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