Mouse Protein delta homolog 1(DLK1) ELISA kit

Instructions
Code CSB-EL006945MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name delta-like 1 homolog (Drosophila)
Alternative Names Dlk1 ELISA kit; Dlk ELISA kit; Pref1 ELISA kit; Scp-1Protein delta homolog 1 ELISA kit; DLK-1 ELISA kit; Adipocyte differentiation inhibitor protein ELISA kit; Preadipocyte factor 1 ELISA kit; Pref-1) [Cleaved into: Fetal antigen 1 ELISA kit; FA1)] ELISA kit
Abbreviation DLK1
Uniprot No. Q09163
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse DLK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %84-96
1:2Average %95
Range %91-99
1:4Average %94
Range %90-98
1:8Average %102
Range %92-108
Recovery
The recovery of mouse DLK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9692-102
EDTA plasma (n=4)8982-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.045 1.944 1.995 1.844
5001.644 1.523 1.584 1.433
2501.073 1.052 1.063 0.912
1250.673 0.662 0.668 0.517
62.50.371 0.382 0.377 0.226
31.20.284 0.273 0.279 0.128
15.60.235 0.231 0.233 0.082
00.148 0.153 0.151  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.
Gene References into Functions
  1. Functional analysis revealed that ground-state miRNAs embedded in the Dlk1-Dio3 locus (miR-541-5p, miR-410-3p, and miR-381-3p) promoted pluripotency via inhibition of multi-lineage differentiation and stimulation of self-renewal PMID: 29129685
  2. This study suggests that Pref-1 is an endocrine factor which is synergistically increased by obesity and age. PMID: 29138005
  3. Gene body methylation of noncoding RNA genes was observed and among these microRNA genes were prominent. Of particular note, observed only in hyperphenylalaninemic animals, was hypomethylation of miRNA genes within the imprinted Dlk1-Dio3 locus on chromosome 12. PMID: 26822703
  4. The role of elevated oxygen levels in eroding imprinted Dlk1 expression during prolonged culture and in vitro differentiation. PMID: 27729406
  5. Loss of DLK1 expression is associated with fetal growth retardation complications of pregnancy. PMID: 27776119
  6. Pref-1 mRNA is a novel substrate of RNase-L. PMID: 27831565
  7. the conserved sequences in IG-DMR are involved in the expression regulation of some of the imprinted genes in the Dlk1-Dio3 domain PMID: 27904015
  8. To investigate the nature of these more variably methylated secondary differentially methylated regions, we adopted a hairpin linker bisulfite mutagenesis approach to examine CpG dyad methylation at differentially methylated regions associated with the murine Dlk1/Gtl2 imprinting cluster on both complementary strands. PMID: 28649282
  9. NOTCH1 ligand Delta-like 1 homolog (DLK1) self interacts PMID: 28099888
  10. the salivary gland phenotype of Dlk1 knock-out mice, was investigated. PMID: 26711912
  11. The brain-specific miR-379/miR-410 gene cluster at the imprinted Dlk1-Dio3 domain is implicated in several aspects of brain development and function. PMID: 26744330
  12. data provide the first evidence for a direct interaction between DLK1 and NOTCH1 in mammals, and substantiate that non-canonical NOTCH ligands exist, adding to the complexity of NOTCH signaling. PMID: 26791579
  13. the Dlk1-Gtl2 locus plays a critical role in preserving long-term repopulating HSCs (LT-HSCs). PMID: 26627594
  14. DNA methylation regulates genomic imprinted DLK1-Dio3 miRNAs in autoimmune lupus PMID: 27070142
  15. identify the molecular regulators involved in the recruitment of AFF3 to gDMRs and provide mechanistic insight into the requirement of AFF3 at an enhancer for the expression of an approximately 200-kb polycistronic transcript within the imprinted Dlk1-Dio3 locus PMID: 26728555
  16. Dppa3 has a critical role in generation of fully reprogrammed iPS cells and maintenance of Dlk1-Dio3 imprinting PMID: 25613421
  17. results suggest that maternal expression of the imprinted miR-379/miR-544 cluster regulates paternal expression of the Dlk1 gene in mice PMID: 26487685
  18. Dll1 and Jag1, Notch ligands, function redundantly and are necessary to maintain the centroacinar cells as an environmental niche in the developing pancreas. PMID: 25919081
  19. Gtl2((-/+)) teratomas have decreased expression of 28 miRNAs encoded by the Dlk1-Dio3 domain PMID: 25355544
  20. DLK1 is a novel inflammatory inhibitor which interferes with NOTCH1 signaling in TLR-activated murine macrophages. PMID: 26115479
  21. these data suggest that miR-143 promotes adipogenesis by directly modulating the pref-1 expression in adipocytes. PMID: 25366176
  22. This study provides the spatiotemporal expression and dynamic changes of lncRNAs from Dlk1-Dio3 imprinted region in mouse preimplantation stage embryos and offers insight into the potential mechanisms responsible for Gtl2 activation. PMID: 26005002
  23. Pref-1 expressing cells are required for adipose tissue development. PMID: 25088414
  24. Data indicate that systemic delta-like 1 protein Dll1/delta-like4 protein Dll4 inhibition decreased T cell cytokines and graft infiltration. PMID: 25687759
  25. the function of DLK1 is to shift the metabolic mode of the organism toward peripheral lipid oxidation and away from lipid storage PMID: 25349437
  26. DNA methylation dynamics of a maternally methylated DMR in the mouse Dlk1-Dio3 domain PMID: 25447521
  27. Multiple Dlk1 splice variants are expressed during early mouse embryogenesis. PMID: 24860997
  28. DLK1 regulates submandibular salivary glands morphogenesis and parasympathetic nerve fibre outgrowth through inhibition of NOTCH signalling PMID: 24828459
  29. Upregulation of Prdm14 results in a highly homogeneous population of authentic pluripotent colonies and prevents the abnormal silencing of the Dlk1-Dio3 locus. PMID: 24552707
  30. Study characterized in detail AK044800, a transcript from the Dlk1-Dio3 imprinted region. PMID: 23515577
  31. Dlk1 isoforms have distinctive effects on myogenesis, and its regulation during myogenesis is critical for normal muscle development. PMID: 24582655
  32. Dlk1 is both necessary and sufficient for determining fast motor neurons and their corresponding biophysical signature in the mouse and chick. PMID: 24626931
  33. Dlk1 is a negative regulator of emerging hematopoietic stem and progenitor cells. PMID: 22801971
  34. delta-like 1 homolog (Dlk1), an imprinted gene best known for its ability to inhibit adipogenesis, is a crucial regulator of the myogenic program in skeletal muscle. PMID: 23946446
  35. data suggest that loss of Dlk1 gives rise to minor pituitary defects manifesting as an age- and sex-dependent reduction in pituitary hormone contents PMID: 23279263
  36. data identify Dlk1-Dio3 ncRNAs as novel candidate early biomarkers for mouse liver tumor promotion and provide new opportunities for assessing the carcinogenic potential of novel compounds PMID: 23091169
  37. Dlk1 expression is not essential for the normal development of beta-cells, somatotrophs and endothelial cells. PMID: 23059197
  38. Data indicate a set of 29 up-regulated miRNAs originating from the Dlk1-Dio3 genomic imprinted region, which has been identified as a hallmark of pluripotency and proliferation. PMID: 23554452
  39. Data indicate that DLK+ fetal hepatic progenitors cells support long-term expansion of hematopoietic stem cells (HSCs). PMID: 23415675
  40. Data also found that Dlk interrupts the binding between Cfr and Fgf18. The Fgf18 signaling pathway seems to be finely tuned by Cfr and Dlk for skeletal development. PMID: 20023171
  41. Data found that upon liver injury, Dlk1 was dramatically induced and initially expressed in hepatocytes and then into the HSCs by a paracrine manner. PMID: 21239501
  42. Data suggest that only membrane-bound Dlk1 (Dlk1-M) represses preadipocyte proliferation, not soluble form (Dlk1-S). Adult Dlk1-null mice exhibit higher amounts of fat than wildtype control mice, possibly due to increased preadipocyte replication. PMID: 22891218
  43. localize the majority of Dlk1 cis-regulatory elements to a 41 kb region upstream of the gene PMID: 22606264
  44. DLK1 may have a dual role in the hypothalamus: it may play a role in the post-natal hypothalamic maturation of the arginine vasopressin and oxytocin neurons. PMID: 22563444
  45. the imprinted delta-like homolog 1/preadipocyte factor (Dlk1/Pref1) and iodothyronine deiodinase type 3 (Dio3) functions converge on the development of brown fat at the transition to independent life. PMID: 22326222
  46. crosstalk between TGF-beta1 and Dlk1 was shown to promote early chondrogenesis during the embryonic endochondral ossification process. PMID: 22102178
  47. Dlk1-null mice showed increased levels of NOTCH downstream targets, such as Snail and Slug PMID: 22068159
  48. novel roles of HSC-derived DLK1 in activating HSCs via epigenetic Ppargamma repression and participating in liver regeneration and development in a manner involving the mesenchymal-epithelial interaction. PMID: 22298767
  49. These findings provide evidence supporting several regulatory functions of delta-like protein 1 (DLK1) in the pituitary gland. PMID: 21756269
  50. Dlk1/FA1 acts as an inhibitor of the PI3K/Akt pathways that leads to its inhibitory effects on chondrogenesis. PMID: 21724852

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Subcellular Location Membrane, Single-pass type I membrane protein
Tissue Specificity Highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary and, to a lesser degree, in adult kidney, muscle, thymus and heart.
Database Links

KEGG: mmu:13386

STRING: 10090.ENSMUSP00000063104

UniGene: Mm.157069

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