Mouse Secretoglobin family 3A member 2(SCGB3A2) ELISA kit

Code CSB-EL020819MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
secretoglobin, family 3A, member 2
Alternative Names
Scgb3a2 ELISA Kit; Pnsp1 ELISA Kit; Ugrp1 ELISA Kit; Secretoglobin family 3A member 2 ELISA Kit; Pneumo secretory protein 1 ELISA Kit; PnSP-1 ELISA Kit; Uteroglobin-related protein 1 ELISA Kit
Abbreviation
SCGB3A2
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
9.4 pg/mL-600 pg/mL
Sensitivity
2.35 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Epigenetics and Nuclear Signaling
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SCGB3A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 82-91
1:2 Average % 94
Range % 89-98
1:4 Average % 107
Range % 103-110
1:8 Average % 110
Range % 104-114
Recovery
The recovery of mouse SCGB3A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 91-99
EDTA plasma (n=4) 103 98-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
600 2.511 2.468 2.490 2.318
300 1.672 1.699 1.686 1.514
150 1.015 0.989 1.002 0.830
75 0.594 0.586 0.590 0.418
37.5 0.403 0.412 0.408 0.236
18.75 0.292 0.289 0.291 0.119
9.4 0.218 0.207 0.213 0.041
0 0.171 0.173 0.172  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse SCGB3A2 ELISA Kit was designed for the quantitative measurement of Mouse SCGB3A2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 9.4 pg/mL-600 pg/mL and the sensitivity is 2.35 pg/mL.

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Target Background

Function
(From Uniprot)
Secreted cytokine-like protein. Binds to the scavenger receptor MARCO. Can also bind to pathogens including the Gram-positive bacterium L.monocytogenes, the Gram-negative bacterium P.aeruginosa, and yeast. Strongly inhibits phospholipase A2 (PLA2G1B) activity. Seems to have anti-inflammatory effects in respiratory epithelium. Also has anti-fibrotic activity in lung. May play a role in fetal lung development and maturation. Promotes branching morphogenesis during early stages of lung development. In the pituitary, may inhibit production of follicle-stimulating hormone (FSH) and luteinizing hormone (LH).
Gene References into Functions
  1. These results demonstrate that SCGB3A2 has an anti-inflammatory activity in the HDM-induced allergic airway inflammation model, in which SCGB3A2 may modulate the CCR4-CCL17 pathway. PMID: 27820933
  2. The pulmonary fibrosis in the Scgb3a2-null mice was more severe than the wild-type controls, thus establishing that SCGB3A2 has anti-fibrotic activity in vivo. PMID: 26559674
  3. SCGB3A2 is an anti-fibrotic agent, and suggest a possible therapeutic use of recombinant SCGB3A2 in the treatment of pulmonary fibrosis. PMID: 26178733
  4. The results indicate that the loss of SCGB3A2 function was influenced by a modifier gene(s) in mixed genetic background and suggest that SCGB3A2 has anti-inflammatory property. PMID: 25242865
  5. SCGB3A2 regulates FSH/LH production in the anterior pituitary lobe. PMID: 24514953
  6. Secretoglobin 3A2 suppresses bleomycin-induced pulmonary fibrosis by transforming growth factor beta signaling down-regulation PMID: 21478551
  7. Results demonstrate that SCGB3A2 is a useful marker for diagnosis of pulmonary tumors both in mice and humans. PMID: 20466451
  8. UGRP1 does not play a major role in the development of bronchial asthma in our Caucasian population. PMID: 12915772
  9. results demonstrate that interleukin-10 induces uteroglobin related protein 1(UGRP1) gene expression in lung epithelial cells through transcription factor T/EBP/NKX2.1-dependent pathway PMID: 15485815
  10. UGRP1 is downregulated in inflamed airways, such as allergic asthmatics. PMID: 15531759
  11. To assess a potential role for SCGB3A2 during early lung development, wild-type and Nkx2-1-null fetal lungs of early developmental stages were subjected to ex vivo organ culture in the presence of SCGB3A2. PMID: 18535256
  12. C/EBPalpha and C/EBPdelta have a role in the synergistic interaction with NKX2-1 in mouse Scgb3a2 gene transcription and lung development PMID: 18632661
  13. SCGB3A2 gene may contribute to Graves' disease susceptibility. PMID: 19126779

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Subcellular Location
Secreted.
Protein Families
Secretoglobin family, UGRP subfamily
Tissue Specificity
Highly expressed in lung where it localizes to epithelial cells of the trachea, bronchus and bronchioles (at protein level). Expressed in club/Clara cells of the bronchioles. Also detected in the anterior and posterior lobes of the pituitary gland where i
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