Mouse Serine protease HTRA1(HTRA1) ELISA kit

Code CSB-EL010901MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
HtrA serine peptidase 1
Alternative Names
Htra1 ELISA Kit; Htra ELISA Kit; Prss11 ELISA Kit; Serine protease HTRA1 ELISA Kit; EC 3.4.21.- ELISA Kit; High-temperature requirement A serine peptidase 1 ELISA Kit; Serine protease 11 ELISA Kit
Abbreviation
HTRA1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
78 pg/mL-5000 pg/mL
Sensitivity
19.5 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HTRA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 85-93
1:2 Average % 95
Range % 90-100
1:4 Average % 91
Range % 86-95
1:8 Average % 97
Range % 92-101
Recovery
The recovery of mouse HTRA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 100-110
EDTA plasma (n=4) 93 89-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
5000 2.635 2.492 2.564 2.374
2500 2.001 1.903 1.952 1.762
1250 1.329 1.357 1.343 1.153
625 0.814 0.860 0.837 0.647
312 0.547 0.519 0.533 0.343
156 0.352 0.338 0.345 0.155
78 0.263 0.271 0.267 0.077
0 0.186 0.194 0.190  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse HTRA1 ELISA Kit was designed for the quantitative measurement of Mouse HTRA1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, but it is unclear whether it leads to the proteolytic degradation of TGF-beta proteins themselves or not. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.
Gene References into Functions
  1. transfection of cells with a constitutively active rapamycin-resistant p70S6K mutant could restore the mineralizing capacity of HtrA1-deficient mouse adipose-derived stromal cells. PMID: 26950191
  2. HtrA1 plays important roles in the differentiation of trophoblasts from Tpbpa-positive precursors in the ectoplacental cone PMID: 25446274
  3. The HtrA1 overexpression and mainstream cigarette smoke can independently lead to CNV. The HtrA1 gene is a strong risk factor for wet AMD PMID: 25205867
  4. the inhibitory effect of IFN-gamma on HTRA1 expression was evidenced in collagen-induced arthritis (CIA) mouse models and in human RA synovial cells. PMID: 24907345
  5. Serine protease HTRA1 antagonizes transforming growth factor-beta signaling by cleaving its receptors and loss of HTRA1 in vivo enhances bone formation. PMID: 24040176
  6. This study offers new insights into the regulation of HTRA-1 expression via LPS/TLR-4 and the role of HTRA-1 in rheumatoid arthrisis pathogenesis. PMID: 23982886
  7. suggest a critical role for LRP1 in maintaining the integrity of vessels by regulating protease activity as well as matrix deposition by modulating HtrA1 and connective tissue growth factor protein levels. PMID: 23868935
  8. a critical role of HTRA1 in the regulation of angiogenesis via TGF-beta signaling and identified GDF6 as a novel disease gene for AMD. PMID: 22049084
  9. showed that increased HTRA1 induced cardinal features of polypoidal choroidal vasculopathy, including branching networks of choroidal vessels, polypoidal lesions, severe degeneration of the elastic laminae, and tunica media of choroidal vessels PMID: 21844367
  10. HtrA1 may disrupt the pericellular matrix network, resulting in alteration of chondrocyte metabolisms. This eventually leads to osteoarthritis. PMID: 20238298
  11. HtrA1 has a role in promoting anoikis by attenuating activation of EGFR/AKT pathway that may contribute to its metastasis suppression capacity PMID: 20388781
  12. During embryo development, HtrA1 was expressed in specific areas where signaling by Tgfbeta family proteins plays important regulatory roles PMID: 14973287
  13. HtrA1 as a protease potentially important for trophoblast differentiation/invasion and uterine decidual regression during placental development PMID: 15861393
  14. bone matrix shows a high level of HtrA1 protein deposition akin to that of TGF-beta, suggesting a close functional interaction between TGF-beta and HtrA1 PMID: 15993670
  15. highly upregulated during mucosal mast cell differentiation PMID: 17292962
  16. HtrA1 and PRSS35 may be involved in ovulation and corpus luteum formation and regression, and that PRSS23 may play a role in follicular atresia PMID: 18566130

Show More

Hide All

Subcellular Location
Cell membrane. Secreted. Cytoplasm, cytosol.
Protein Families
Peptidase S1C family
Tissue Specificity
In the brain, mainly expressed in cortical areas both in glial cells and neurons (at protein level). In bones, deposited in the matrix, with higher level in newly formed bone compared to fully calcified bone (at protein level). Also expressed in the tendo
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X