Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Htra1

Uniprot No.

Q9R118

Research Area

Signal Transduction

Alternative Names

Htra1; Htra; Prss11; Serine protease HTRA1; EC 3.4.21.-; High-temperature requirement A serine peptidase 1; Serine protease 11

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

141-480aa

Target Protein Sequence

KLRQPPVIVLQRGACGQGQEDPNSLRHKYNFIADVVEKIAPAVVHIELYRKLPFSKREVPVASGSGFIVSEDGLIVTNAHVVTNKNRVKVELKNGATYEAKIKDVDEKADIALIKIDHQGKLPVLLLGRSSELRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDKIKKFLTESHDRQAKGKAVTKKKYIGIRMMSLTSSKAKELKDRHRDFPDVLSGAYIIEVIPDTPAEAGGLKENDVIISINGQSVVTANDVSDVIKKENTLNMVVRRGNEDIVITVIPEEIDP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

39.0 kDa

Protein Length

Partial

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The process of expressing the recombinant mouse Htra1 protein in the E.coli requires the recombinant DNA gene formed by the integration of encoding gene for the 141-480aa of the mouse Htra1 protein and C-terminal 6xHis tag sequence, the expression vector that the recombinant DNA gene inserts into, the E.coli that provided the necessary macromolecules and components for transcription and translation of the cloned expression vector. After isolation and purification, this C-terminal 6xHis-tagged recombinant Htra1 protein was obtained. This recombinant Htra1 protein is characterized by high purity (>85%, SDS-PAGE). This Htra1 protein ran along the gel to the band of approximately 43 kDa molecular weight.

Serine protease HTRA1 is a protein encoding by a gene named Htra1 in mouse and a gene named HTRA1 in human. This protein, a member of serine proteases, which has an active center that cuts (cleaves) other proteins into smaller pieces. It consists of four well-defined domains, including an IGFBP domain, a Kazal domain, a protease domain and a PDZ domain. HTRA1 is a secretory protein and also present intracellularly and associated with microtubules. The proteolytic activity of HTRA1 regulates a broad range of physiological processes involving in bone biology, osteoarthritis, intervertebral disc (IVD) degeneration and tumorigenesis.

Target Background

Function

Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, but it is unclear whether it leads to the proteolytic degradation of TGF-beta proteins themselves or not. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.

Gene References into Functions

transfection of cells with a constitutively active rapamycin-resistant p70S6K mutant could restore the mineralizing capacity of HtrA1-deficient mouse adipose-derived stromal cells. PMID: 26950191
HtrA1 plays important roles in the differentiation of trophoblasts from Tpbpa-positive precursors in the ectoplacental cone PMID: 25446274
The HtrA1 overexpression and mainstream cigarette smoke can independently lead to CNV. The HtrA1 gene is a strong risk factor for wet AMD PMID: 25205867
the inhibitory effect of IFN-gamma on HTRA1 expression was evidenced in collagen-induced arthritis (CIA) mouse models and in human RA synovial cells. PMID: 24907345
Serine protease HTRA1 antagonizes transforming growth factor-beta signaling by cleaving its receptors and loss of HTRA1 in vivo enhances bone formation. PMID: 24040176
This study offers new insights into the regulation of HTRA-1 expression via LPS/TLR-4 and the role of HTRA-1 in rheumatoid arthrisis pathogenesis. PMID: 23982886
suggest a critical role for LRP1 in maintaining the integrity of vessels by regulating protease activity as well as matrix deposition by modulating HtrA1 and connective tissue growth factor protein levels. PMID: 23868935
a critical role of HTRA1 in the regulation of angiogenesis via TGF-beta signaling and identified GDF6 as a novel disease gene for AMD. PMID: 22049084
showed that increased HTRA1 induced cardinal features of polypoidal choroidal vasculopathy, including branching networks of choroidal vessels, polypoidal lesions, severe degeneration of the elastic laminae, and tunica media of choroidal vessels PMID: 21844367
HtrA1 may disrupt the pericellular matrix network, resulting in alteration of chondrocyte metabolisms. This eventually leads to osteoarthritis. PMID: 20238298
HtrA1 has a role in promoting anoikis by attenuating activation of EGFR/AKT pathway that may contribute to its metastasis suppression capacity PMID: 20388781
During embryo development, HtrA1 was expressed in specific areas where signaling by Tgfbeta family proteins plays important regulatory roles PMID: 14973287
HtrA1 as a protease potentially important for trophoblast differentiation/invasion and uterine decidual regression during placental development PMID: 15861393
bone matrix shows a high level of HtrA1 protein deposition akin to that of TGF-beta, suggesting a close functional interaction between TGF-beta and HtrA1 PMID: 15993670
highly upregulated during mucosal mast cell differentiation PMID: 17292962
HtrA1 and PRSS35 may be involved in ovulation and corpus luteum formation and regression, and that PRSS23 may play a role in follicular atresia PMID: 18566130

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Subcellular Location

Cell membrane. Secreted. Cytoplasm, cytosol.

Protein Families

Peptidase S1C family

Tissue Specificity

In the brain, mainly expressed in cortical areas both in glial cells and neurons (at protein level). In bones, deposited in the matrix, with higher level in newly formed bone compared to fully calcified bone (at protein level). Also expressed in the tendo

Database Links

KEGG: mmu:56213

STRING: 10090.ENSMUSP00000006367

UniGene: Mm.30156

Code	CSB-EP879501MO1
Abbreviation	Recombinant Mouse Htra1 protein, partial
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP879501MO1 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Htra1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP879501MO1 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Htra1.
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Recombinant Mouse Serine protease HTRA1 (Htra1), partial

Product Details

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