Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Htra1 Polyclonal antibody

Uniprot No.

Q9R118

Target Names

Htra1

Alternative Names

Htra1 antibody; Htra antibody; Prss11 antibody; Serine protease HTRA1 antibody; EC 3.4.21.- antibody; High-temperature requirement A serine peptidase 1 antibody; Serine protease 11 antibody

Raised in

Rabbit

Species Reactivity

Mus musculus

Immunogen

Recombinant Mouse Serine protease HTRA1 (Htra1), partial (141-480AA)

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein A/G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The production of the Htra1 polyclonal antibody is a systematic process that begins with the repeated immunization of a rabbit using recombinant mouse serine protease Htra1 protein (141-480aa). This immunization continues until a sufficient antibody titer is achieved. Subsequently, the rabbit's blood is collected, and the antibodies are purified from the serum using protein A/G. The functionality of the resulting Htra1 antibody is rigorously assessed in ELISA and WB applications, confirming its specific reactivity with the mouse Htra1 protein.

In the extracellular space, Htra1 functions as a serine protease that degrades a number of proteins and extracellular matrix molecules. It contributes to protein quality control, recognizing and cleaving abnormal proteins into smaller fragments to prevent misfolding and mislocalization. Intracellularly, Htra1 is involved in tumor suppression and cell proliferation and migration. Htra1 thus plays a role in several diseases such as macular degeneration, arthritis, and CARASIL.

Target Background

Function

Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, but it is unclear whether it leads to the proteolytic degradation of TGF-beta proteins themselves or not. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.

Gene References into Functions

transfection of cells with a constitutively active rapamycin-resistant p70S6K mutant could restore the mineralizing capacity of HtrA1-deficient mouse adipose-derived stromal cells. PMID: 26950191
HtrA1 plays important roles in the differentiation of trophoblasts from Tpbpa-positive precursors in the ectoplacental cone PMID: 25446274
The HtrA1 overexpression and mainstream cigarette smoke can independently lead to CNV. The HtrA1 gene is a strong risk factor for wet AMD PMID: 25205867
the inhibitory effect of IFN-gamma on HTRA1 expression was evidenced in collagen-induced arthritis (CIA) mouse models and in human RA synovial cells. PMID: 24907345
Serine protease HTRA1 antagonizes transforming growth factor-beta signaling by cleaving its receptors and loss of HTRA1 in vivo enhances bone formation. PMID: 24040176
This study offers new insights into the regulation of HTRA-1 expression via LPS/TLR-4 and the role of HTRA-1 in rheumatoid arthrisis pathogenesis. PMID: 23982886
suggest a critical role for LRP1 in maintaining the integrity of vessels by regulating protease activity as well as matrix deposition by modulating HtrA1 and connective tissue growth factor protein levels. PMID: 23868935
a critical role of HTRA1 in the regulation of angiogenesis via TGF-beta signaling and identified GDF6 as a novel disease gene for AMD. PMID: 22049084
showed that increased HTRA1 induced cardinal features of polypoidal choroidal vasculopathy, including branching networks of choroidal vessels, polypoidal lesions, severe degeneration of the elastic laminae, and tunica media of choroidal vessels PMID: 21844367
HtrA1 may disrupt the pericellular matrix network, resulting in alteration of chondrocyte metabolisms. This eventually leads to osteoarthritis. PMID: 20238298
HtrA1 has a role in promoting anoikis by attenuating activation of EGFR/AKT pathway that may contribute to its metastasis suppression capacity PMID: 20388781
During embryo development, HtrA1 was expressed in specific areas where signaling by Tgfbeta family proteins plays important regulatory roles PMID: 14973287
HtrA1 as a protease potentially important for trophoblast differentiation/invasion and uterine decidual regression during placental development PMID: 15861393
bone matrix shows a high level of HtrA1 protein deposition akin to that of TGF-beta, suggesting a close functional interaction between TGF-beta and HtrA1 PMID: 15993670
highly upregulated during mucosal mast cell differentiation PMID: 17292962
HtrA1 and PRSS35 may be involved in ovulation and corpus luteum formation and regression, and that PRSS23 may play a role in follicular atresia PMID: 18566130

Hide All

Subcellular Location

Cell membrane. Secreted. Cytoplasm, cytosol.

Protein Families

Peptidase S1C family

Tissue Specificity

In the brain, mainly expressed in cortical areas both in glial cells and neurons (at protein level). In bones, deposited in the matrix, with higher level in newly formed bone compared to fully calcified bone (at protein level). Also expressed in the tendo

Database Links

KEGG: mmu:56213

STRING: 10090.ENSMUSP00000006367

UniGene: Mm.30156

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Code	CSB-PA879501ZA01MO
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: Htra1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 kDa Observed band size: 40 kDa
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Htra1 Antibody

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