Mouse Syndecan-1(SDC1) ELISA kit

Code CSB-EL020888MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
syndecan 1
Alternative Names
Sdc1; Synd-1; Synd1; Syndecan-1; SYND1; CD antigen CD138
Abbreviation
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-97  
1:2 Average % 97  
Range % 93-99  
1:4 Average % 92  
Range % 88-96  
1:8 Average % 94  
Range % 90-98  
Recovery
The recovery of mouse SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-95  
EDTA plasma (n=4) 95 91-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.844 2.872 2.858 2.771  
2000 1.987 2.007 1.997 1.910  
1000 1.132 1.138 1.135 1.048  
500 0.631 0.620 0.626 0.539  
250 0.364 0.355 0.360 0.273  
125 0.241 0.236 0.239 0.152  
62.5 0.158 0.152 0.155 0.068  
0 0.088 0.086 0.087    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse SDC1 ELISA Kit was designed for the quantitative measurement of Mouse SDC1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .

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Target Background

Function
(From Uniprot)
Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
Gene References into Functions
  1. Based on our results, we conclude that SDC1 on DC negatively regulates DC migration. PMID: 28453867
  2. findings support a key role for Sdc1 in modulating pulmonary protection by FFP after hemorrhagic shock. PMID: 28107214
  3. 2-O-sulfated domains in syndecan-1 heparan sulfate halt disease progression and promote liver repair by enhancing hepatocyte survival in acetaminophen-induced liver injury PMID: 28543100
  4. CD138 expression on fully mature Antibody secreting cells (ASCs) provides a selective survival advantage over less mature, newly minted ASCs, by enhancing pro-survival cytokine signaling. PMID: 28381397
  5. Sdc1 deficiency results in increased susceptibility to colitis-associated tumorigenesis. PMID: 28350804
  6. MMP7 shedding of syndecan-1/CXCL1 complexes functions as a checkpoint that restricts neutrophil activation at sites of epithelial injury. PMID: 26934670
  7. Data show that heparanase-1 (HPA-1) induced shedding of heparan sulfate chain from syndecan-1 (SDC-1) facilitated the release of vascular endothelial growth factor C (VEGF-C) from SDC-1/VEGF-C complex into the medium of hepatocarcinoma cell. PMID: 28209511
  8. This study indicates that, while syndecan-1 is important for providing a barrier to acute S. aureus infection in PD, it does not affect peritoneal fibrosis and angiogenesis. PMID: 26832929
  9. Data suggest a potential mechanism of diabetic enteropathy, which is depending remarkably on syndecan-1 (Sdc1) and -beta-D-glucuronidase heparanase (HPSE). PMID: 25702768
  10. Study showed the expression, distribution and function of syndecan-1 in primary sensory neurons after nerve injury PMID: 26002314
  11. A transmembrane C-terminal fragment of syndecan-1 is generated by the metalloproteinase ADAM17 and promotes lung epithelial tumor cell migration and lung metastasis formation. PMID: 25912030
  12. The PPARgamma agonist rosiglitazone rescues Sdc1-/- intradermal adipose tissue, placing PPARgamma downstream of Sdc1 in triggering adipocyte differentiation PMID: 25101993
  13. no expression within the vasculature PMID: 25972509
  14. Specific structural motifs in syndecan-1 HS promote Staphylococcus aureus corneal infection by inhibiting neutrophil CRAMP. PMID: 25931123
  15. These results demonstrate that defective motility in Sdc-1(-/-) macrophages promotes a persistent inflammatory state with relevance to the pathogenesis of atherosclerosis. PMID: 25550207
  16. Sdc1 affects arteriogenesis in response to hindlimb ischemia and is required in the local tissue environment for normal arteriogenesis. PMID: 25194470
  17. Syndecan-1 promotes vascular smooth muscle cell differentiation and quiescence. PMID: 24587062
  18. The conserved domain 2 (C2) and variable (V) regions of syndecan- 1 cytoplasmic tail mediate heparanase processing. PMID: 24788042
  19. These data show that CD138(+) cells, induced by treatment with CS, migrate into the bone marrow and may displace other antigen-specific plasma cells. PMID: 24555985
  20. studies support a key role for sdc-1 in endothelial mechanosensing and regulation of endothelial phenotype. PMID: 24554698
  21. In Sdc-1(-/-) mice, early leukocyte recruitment via the choroid plexus is enhanced, and IL-6 is elevated, which collectively results in higher numbers of the disease inducing Th17 cells in the CNS, thereby contributing to enhanced disease severity. PMID: 24078687
  22. high expression of the ppGalNAc-T13 gene generates tTn antigen on Syndecan 1, leading to enhanced invasion and metastasis via the formation of a complex consisting of integrin alpha5beta1, Syndecan 1, and MMP-9 in the glycolipid-enriched microdomain/rafts. PMID: 23814067
  23. Syndecan-1 does not appear to be a prerequisite for the existence of an endothelial glycocalyx. PMID: 23428342
  24. early differentiated CD138(high)MHCII(+) rather than terminally differentiated CD138(high)MHCII(low) plasma cells may be involved in the renal inflammatory injury in lupus, due to CXCR3 expression and IgG secretion. PMID: 23520491
  25. Clearance of triglyceride-rich lipoproteins by hepatic SDC1 is atheroprotective and mediated by multivalent binding to ApoE and ApoAV. PMID: 23676495
  26. These results suggest that insulin stimulates the association of insulin receptor with syndecan-1 and the complex formation of syndecan-1 and integrin could play an important role in ERK I/II-ALP signaling pathway in osteoblast cells. PMID: 23252577
  27. Sdc1 in the germinal niche is a key HSPG regulating the maintenance and proliferation of NPCs during cortical neurogenesis, in part by modulating the ability of NPCs to respond to Wnt ligands. PMID: 22936997
  28. The loss of syndecan-1 expression in the epithelial cell membrane is associated with tumor cell growth and invasiveness PMID: 22686587
  29. These results suggest that syndecan-1 may have a crucial role in the survival of injured motor neurons and in nerve regeneration after injury. PMID: 22944346
  30. Data suggest a mechanism for the inhibitory effects of Sdc-1 on cell growth that involves the interaction between the cytoplasmic domain of Sdc-1 and the SUMO-1 E3 ligase, Topors. PMID: 22912899
  31. did not see a significant reduction in reporter-gene transduction by papillomaviruses in syndecan-1 null mice PMID: 22995187
  32. the lung epithelium requires the syndecan-1 transmembrane domain to govern cell migration and is independent from its ability to control cell adhesion via the extracellular domain. PMID: 22936802
  33. The 1.5-kb upstream region of the syndecan-1 gene was sufficient to induce its expression in dental papilla, and binding of Sp3 transcription factor may play a pivotal role in this syndecan-1 induction. PMID: 22134060
  34. Reduced Syndecan-1 is associated with severe colitis in IL-10 knockout mice. PMID: 21987406
  35. These results suggest that syndecan 1 plays a novel role in the protective effects of enteral glutamine in the postischemic gut. PMID: 22706022
  36. Data show that wildtype-syndecan-1(WT-sdc1) and unshedding-syndecan-1(uS-sdc1) are successfully constructed. PMID: 22482411
  37. syndecan-1 promotes tubular survival and repair in ischemia/reperfusion injury PMID: 22237752
  38. The myocardial levels of expression of Sdc1 mRNA and protein increased significantly in myocardial infarction(MI); The expression of soluble Sdc1 in the sera of the rats in the MI group followed a similar pattern PMID: 21842422
  39. Heparan sulfate chains of syndecan-1 regulate ectodomain shedding. PMID: 22298773
  40. Molecular functions of syndecan-1 in disease.(review) PMID: 22033227
  41. Bacillus anthracis infection contribute to the formation of thrombi consisting of aggregated bacteria, vWF, shed SDC-1, fibrin, activated platelets and fibronectin. PMID: 22001909
  42. Syndecan-1 displays a protective role in aortic aneurysm formation by modulating T cell-mediated responses. PMID: 22173227
  43. Heparanase-mediated loss of nuclear syndecan-1 enhances histone acetyltransferase (HAT) activity to promote expression of genes that drive an aggressive tumor phenotype PMID: 21757697
  44. SST0001, a chemically modified heparin, inhibits myeloma growth and angiogenesis via disruption of the heparanase/syndecan-1 axis PMID: 21257720
  45. Sdc1 shedding is activated in colitis. Heparin, which mimics Sdc1 functions, relieves colitis severity by inhibiting Sdc1 shedding and downregulating cytokines expression. PMID: 20936359
  46. previously unknown bacterial subversion mechanism where S. aureus exploits the capacity of syndecan-1 ectodomains to inhibit neutrophil-mediated bacterial killing mechanisms in an heparan sulfate-dependent manner to promote its pathogenesis in the cornea PMID: 21127056
  47. the loss of syndecan1 (sdc1) in corneal stromal cells (CSC) impacts cell migration rates, the sizes and composition of focal and fibrillar adhesions, the activation of integrins, and the assembly of fibronectin into fibrils PMID: 20580707
  48. Zoledronic acid can inhibit the growth of KM3 cells in a dose-dependent manner and inhibit CD138 expression. PMID: 19642368
  49. Sdc1 has a protective effect during experimental colitis. PMID: 20008145
  50. MMP7 shedding of syndecan-1 facilitates wound closure by causing the alpha(2)beta(1) integrin to assume a less active conformation thereby removing restrictions to migration PMID: 19668337

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Subcellular Location
Membrane; Single-pass type I membrane protein. Secreted. Secreted, extracellular exosome.
Protein Families
Syndecan proteoglycan family
Database Links
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