Mouse Troponin T, cardiac muscle(TNNT2) ELISA kit

Code CSB-EL024016MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P50752
Lead Time 7-14 working days
Abbreviation TNNT2
Target Name troponin T type 2 (cardiac)
Alias CMH2, CMPD2, MGC3889, RCM3, TnTC, cTnT, OTTHUMP00000033864|OTTHUMP00000033867|cardiomyopathy, dilated 1D (autosomal dominant)|cardiomyopathy, hypertrophic 2|troponin T type 2, cardiac|troponin T, ca
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.
HGNC 11949
RGD 3882
MGI 104597
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNNT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 89-102  
1:2 Average % 91  
Range % 82-98  
1:4 Average % 93  
Range % 85-100  
1:8 Average % 92  
Range % 88-100  
Recovery
The recovery of mouse TNNT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 90-100  
EDTA plasma (n=4) 92 87-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 1.912 1.927 1.920 1.749  
1000 1.513 1.569 1.541 1.370  
500 1.075 1.138 1.107 0.936  
250 0.804 0.798 0.801 0.630  
125 0.511 0.537 0.524 0.353  
62.5 0.349 0.356 0.353 0.182  
31.25 0.254 0.245 0.250 0.079  
0 0.172 0.169 0.171    
References
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Function Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Protein Families Troponin T family
Database Links

KEGG: mmu:21956

STRING: 10090.ENSMUSP00000137093

UniGene: Mm.247470

Pathway Cardiac muscle contraction

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