Mouse coagulation factor Ⅺ,FⅪ ELISA Kit

Code CSB-E17818m
Size 96T,5×96T,10×96T
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Product Details

Target Name
coagulation factor Ⅺ,F
Alternative Names
F11Coagulation factor XI ELISA kit; FXI ELISA kit; EC 3.4.21.27 ELISA kit; Plasma thromboplastin antecedent ELISA kit; PTA) [Cleaved into: Coagulation factor XIa heavy chain; Coagulation factor XIa light chain] ELISA kit
Abbreviation
F11
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.47 ng/mL-30 ng/mL
Sensitivity
0.12 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Blood Coagulation
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅪ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %95
Range %90-101
1:200Average %87
Range %84-90
1:400Average %102
Range %98-106
1:800Average %93
Range %89-97
Recovery
The recovery of mouse FⅪ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)9894-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
300.147 0.139 0.143
150.219 0.225 0.222
7.50.345 0.326 0.336
3.750.513 0.536 0.525
1.8750.857 0.862 0.860
0.941.179 1.198 1.189
0.471.538 1.559 1.549
02.533 2.542 2.538
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse F11 ELISA Kit was designed for the quantitative measurement of Mouse F11 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.47 ng/mL-30 ng/mL and the sensitivity is 0.12 ng/mL.

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Target Background

Function
(From Uniprot)
Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
Gene References into Functions
  1. FXI(-/-) mice have a mild hemostatic defect after injury to the saphenous vein but not after transection of the tail or vessels in the abdominal wall. PMID: 28677246
  2. FXI protects against sepsis derived from Streptococcus pneumoniae or Klebsiella pneumoniae pneumonia at least in part by enhancing the phagocytic capacity of neutrophils by a mechanism that is independent of activation via FXIIa. PMID: 28492700
  3. FXI attenuates part of the allergic response to repeated administration of house dust mite in the airways by a mechanism that is independent of activation via FXII. PMID: 27913422
  4. Thrombin-independent contribution of tissue factor to inflammation and cardiac hypertrophy in a mouse model of sickle cell disease. PMID: 26817955
  5. FXI deprivation was shown to slow down atherogenesis in Apoe knockout mice. PMID: 26800563
  6. anion-binding sites on FXIa that are required for expression of heparin's cofactor activity during protease inhibition by antithrombin are also required for expression of polyP cofactor activity during FXI activation PMID: 24118982
  7. Factor XI antisense oligonucleotides safely prevent thrombus formation on acutely ruptured atherosclerotic plaques in mice. PMID: 24947525
  8. Studies indicate that targeting factor XI (FXI) by knocking out the gene prevents arterial and vein thrombosis. PMID: 23929304
  9. Protective roles for fibrin, tissue factor, plasminogen activator inhibitor-1, and thrombin activatable fibrinolysis inhibitor, but not factor XI, during defense against the gram-negative bacterium Yersinia enterocolitica. PMID: 21724997
  10. fXI and fXII contribute to thrombus formation even when factor VIIa/tissue factor initiates thrombosis. PMID: 20634381
  11. Gene targeting of tissue factor, factor X, and factor VII in mice: their involvement in embryonic development PMID: 11841337
  12. FXI is essential for thrombus formation following FeCl3-induced injury of the carotid artery in the mouse. PMID: 12008966
  13. FIX and FXI deficiency cause similar effects in the carotid artery injury model PMID: 15733058
  14. fXI may have functions in regulation of inflammation or tissue repair distinct from its role in coagulation PMID: 15985536
  15. apolipoprotein E receptor 2 (ApoER2, LRP8), a member of the LDL receptor family, is a platelet receptor for FXI. PMID: 19661487

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Subcellular Location
Secreted.
Protein Families
Peptidase S1 family, Plasma kallikrein subfamily
Database Links
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