Inositol 1,4,5-trisphosphate (IP3) is a key second messenger molecule in cellular signaling pathways. Phospholipase C-mediated hydrolysis of phosphatidylinositol 4,5-bisphosphate generates IP3, which plays a fundamental role in intracellular calcium mobilization by binding to IP3 receptors on the endoplasmic reticulum. This interaction triggers calcium release from intracellular stores, initiating downstream signaling cascades necessary for numerous cellular processes including muscle contraction, neurotransmission, and hormone responses. IP3 levels serve as important indicators of cellular activation and are widely studied in research examining signal transduction mechanisms.
The Mouse inositol 1,4,5-trisphosphate ELISA Kit (CSB-E13410m) provides quantitative measurement of IP3 in Mus musculus samples using a sandwich assay principle. This kit accommodates multiple sample types including serum, plasma, cell culture supernatants, tissue homogenates, and cell lysates. The assay offers a detection range of 0.78-50 pg/mL with a sensitivity of 0.195 pg/mL. Detection occurs at 450 nm wavelength, requiring 50-100 μL sample volume and 1-5 hours assay time for completion.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research examining cellular signaling pathways and gastrointestinal physiology. Studies have applied the kit to measure intracellular IP3 levels in various experimental contexts, including tissue analysis and cellular assays.
• Gastrointestinal research: Quantification of IP3 concentrations in colon tissue homogenates as part of studies examining digestive system function and hormone regulation
• Cellular signaling studies: Measurement of intracellular IP3 levels in cultured cells to examine second messenger pathways and cellular responses
• Tissue-based analysis: Determination of IP3 concentrations in homogenized tissue samples to understand local signaling mechanisms and physiological processes
