Human inositol 1,4,5,-trisphosphate(IP3) ELISA kit

Code CSB-E12636h
Size 96T,5×96T,10×96T
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Product Details

Target Name
inositol 1,4,5,-trisphosphate(IP3)
Abbreviation
IP3
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
5 pg/mL-1000 pg/mL
Sensitivity
2.5 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-105  
1:2 Average % 94  
Range % 89-100  
1:4 Average % 94  
Range % 87-102  
1:8 Average % 95  
Range % 90-102  
Recovery
The recovery of human IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 91-100  
EDTA plasma (n=4) 92 87-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1000 0.202 0.211 0.207    
250 0.350 0.349 0.350    
80 0.600 0.594 0.597    
20 1.029 1.018 1.024    
5 1.326 1.345 1.336    
0 2.102 2.130 2.116    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human IP3 ELISA Kit was designed for the quantitative measurement of Human IP3 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 5 pg/mL-1000 pg/mL and the sensitivity is 2.5 pg/mL.

Citations

Customer Reviews and Q&A

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 Q&A
Q:

I would like to receive more information about this assay, particularly regarding specificity for IP3.
Please provide some more information about the following.
a. capture antibody or capture antigen
b. Detection antibody (if applicable)
c. standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.
Are you aware of any product specific references for this assay? If yes please can you provide links to these references. If you have any other information about validation of the antibodies in other applications e.g. WB this information would be helpful also.

A:
Thanks for your inquiry.
CSB-E12636h
This is a competitive kit.
The plate is coated with goat-anti-rabbit antibody.
The specific antibody is rabbit polyclonal antibody.
The standard is purified IP3.
The raw materials of the kit have been screened strictly and passed quality control. Sorry it is not convenient for us to provide validation information of the antibodies because it is only for our internal tracing. There are many references about this kit. Pls find following links:
1. https://www.ncbi.nlm.nih.gov/pubmed/?term=26884398 https://academic.oup.com/humrep/article/31/3/672/2384908
3. https://www.ncbi.nlm.nih.gov/pubmed/26181635
4.https://www.ncbi.nlm.nih.gov/pubmed/25785721
5. https://www.ncbi.nlm.nih.gov/pubmed/23992092
6. https://www.nature.com/articles/nature10246?WT.ec_id=NATURE-20110728
Pls let me know if you have any further questions. Thank you.
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