Human inositol 1,4,5,-trisphosphate(IP3) ELISA kit

Human inositol 1,4,5,-trisphosphate(IP3) ELISA kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Human inositol 1,4,5,-trisphosphate(IP3) ELISA kit

Name: Human inositol 1,4,5,-trisphosphate(IP3) ELISA kit
Brand: CUSABIO
SKU: CSB-E12636h

Citations (10) Protocol

Code	CSB-E12636h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (10)
Related Products
Customer Reviews and Q&A

Target Name

inositol 1,4,5,-trisphosphate(IP3)

Abbreviation

IP3

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

5 pg/mL-1000 pg/mL

Sensitivity

2.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-105
1:2	Average %	94
	Range %	89-100
1:4	Average %	94
	Range %	87-102
1:8	Average %	95
	Range %	90-102

Recovery

The recovery of human IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-100
EDTA plasma (n=4)	92	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.202	0.211	0.207
250	0.350	0.349	0.350
80	0.600	0.594	0.597
20	1.029	1.018	1.024
5	1.326	1.345	1.336
0	2.102	2.130	2.116

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human IP3 ELISA Kit was designed for the quantitative measurement of Human IP3 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 5 pg/mL-1000 pg/mL and the sensitivity is 2.5 pg/mL.

Second messenger role for Mg21 revealed by human T-cell immunodeficiency Feng-Yen Li,Nature,2011
C6-ceramide induces salivary adenoid cystic carcinoma cell apoptosis via IP3R-activated UPR and UPR-independent pathways L Qiu,Biochem. Biophys. Res. Commun,2020
High-dose levocetirizine for the treatment of refractory chronic spontaneous urticaria and the effect on the serum inositol triphosphate level Huang X, et al,Journal of International Medical Research,2019
Activation of calcium signaling in human gingival fibroblasts by recombinant Porphyromonas gingivalis RgpB protein Li S,european journal of oral sciences,2019
Combined assessment of polymorphisms in the LHCGR and FSHR genes predict chance of pregnancy after in vitro fertilization Lindgren I. et al,Hum Reprod,2016
Inositol 1,4,5-trisphosphate (IP3)-dependent Ca2+ signaling mediates delayed myogenesis in Duchenne muscular dystrophy fetal muscle Farini A.et al,Development.,2016
Octopamine Regulates Antennal Sensory Neurons via Daytime-Dependent Changes in cAMP and IP3 Levels in the Hawkmoth Manduca sexta. Schendzielorz T et al,PLoS One,2015
Cilostazol Induces PGI 2 Production via Activation of the Downstream Epac-1/Rap1 Signaling Cascade to Increase Intracellular Calcium by PLC(Epsilon) and to Activate p44/42 MAPK in Human Aortic Endothelial Cells. Hashimoto A et al,PLoS One,2015
Cytochrome C dysregulation induced by HIV infection of astrocytes results in bystander apoptosis of uninfected astrocytes by an IP3 and calcium-dependent mechanism Eugenin EA et al,J Neurochem,2013
Second messenger role for Mg2+ revealed by human T-cell immunodeficiency /,Nature,2011

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■ Q&A

I would like to receive more information about this assay, particularly regarding specificity for IP3.
Please provide some more information about the following.
a. capture antibody or capture antigen
b. Detection antibody (if applicable)
c. standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.
Are you aware of any product specific references for this assay? If yes please can you provide links to these references. If you have any other information about validation of the antibodies in other applications e.g. WB this information would be helpful also.

Thanks for your inquiry.
CSB-E12636h
This is a competitive kit.
The plate is coated with goat-anti-rabbit antibody.
The specific antibody is rabbit polyclonal antibody.
The standard is purified IP3.
The raw materials of the kit have been screened strictly and passed quality control. Sorry it is not convenient for us to provide validation information of the antibodies because it is only for our internal tracing. There are many references about this kit. Pls find following links:
1. https://www.ncbi.nlm.nih.gov/pubmed/?term=26884398 https://academic.oup.com/humrep/article/31/3/672/2384908
3. https://www.ncbi.nlm.nih.gov/pubmed/26181635
4.https://www.ncbi.nlm.nih.gov/pubmed/25785721
5. https://www.ncbi.nlm.nih.gov/pubmed/23992092
6. https://www.nature.com/articles/nature10246?WT.ec_id=NATURE-20110728
Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.