Mouse plasmin-antiplasmin complex,PAP ELISA Kit

Code CSB-E08436m
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
plasmin-antiplasmin complex,PAP
Alternative Names
N/A
Abbreviation
PAP
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 pg/mL-200 pg/mL
Sensitivity
0.78 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 87  
Range % 83-94  
1:10 Average % 97  
Range % 92-102  
1:20 Average % 105  
Range % 98-111  
1:40 Average % 93  
Range % 87-99  
Recovery
The recovery of mouse PAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-96  
EDTA plasma (n=4) 101 95-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.245 2.264 2.255 2.072  
100 1.805 1.855 1.830 1.647  
50 1.178 1.155 1.167 0.984  
25 0.739 0.796 0.768 0.585  
12.5 0.398 0.421 0.410 0.227  
6.25 0.312 0.335 0.324 0.141  
3.12 0.259 0.248 0.254 0.071  
0 0.188 0.178 0.183    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse PAP ELISA Kit was designed for the quantitative measurement of Mouse PAP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

Customer Reviews and Q&A

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 Q&A
Q:

Can you comment on the nature of the antibodies used for the ELISA? Does one of the antibodies exclude the detection of plasminogen?

A:

For CSB-E08436m kit, the target is a compound. There are two different antibodies used in the kit, the capture antibody, and the detection antibody. They are respectively specific to the two parts of the compound and they cannot detect plasminogen.

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