Pig Angiogenin(ANG) ELISA kit

Instructions
Code CSB-EL001703PI
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name angiogenin, ribonuclease, RNase A family, 5
Alternative Names ANGAngiogenin ELISA kit; EC 3.1.27.- ELISA kit; Ribonuclease 5 ELISA kit; RNase 5 ELISA kit
Abbreviation ANG
Uniprot No. P31346
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 94 pg/mL-6000 pg/mL
Sensitivity 47 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 94  
Range % 90-98  
1:200 Average % 96  
Range % 92-99  
1:400 Average % 103  
Range % 97-107  
1:800 Average % 84  
Range % 80-88  
Recovery
The recovery of pig ANG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-98  
EDTA plasma (n=4) 98 94-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
6000 0.129 0.128 0.129    
3000 0.261 0.256 0.259    
1500 0.509 0.529 0.519    
750 0.932 0.941 0.937    
375 1.401 1.394 1.398    
188 1.753 1.742 1.748    
94 2.087 2.007 2.047    
0 2.476 2.554 2.515    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs) (By similarity). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo (By similarity).
Subcellular Location Cytoplasmic vesicle, secretory vesicle lumen, Secreted, Nucleus, nucleolus
Protein Families Pancreatic ribonuclease family
Database Links

STRING: 9823.ENSSSCP00000020122

UniGene: Ssc.11079

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