Pig Complement 3,C3 ELISA Kit

Code CSB-E06920p
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Pig complement component 3 ELISA Kit was designed for the quantitative measurement of Pig complement component 3 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.625 μg/mL-10 μg/mL and the sensitivity is 0.625 μg/mL .

Target Name complement component 3
Alternative Names C3 ELISA Kit; Complement C3 [Cleaved into: Complement C3 beta chain; C3-beta-c ELISA Kit; C3bc); Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein ELISA Kit; ASP ELISA Kit; C3adesArg); Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2] ELISA Kit
Abbreviation C3
Uniprot No. P01025
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 μg/mL-10 μg/mL
Sensitivity 0.625 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 96  
Range % 91-100  
1:200 Average % 93  
Range % 84-103  
1:400 Average % 95  
Range % 91-101  
1:800 Average % 93  
Range % 83-102  
Recovery
The recovery of pig C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 88-108  
EDTA plasma (n=4) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
10 0.083 0.084 0.084    
5 0.185 0.176 0.181    
2.5 0.349 0.352 0.351    
1.25 0.769 0.776 0.773    
0.625 1.285 1.292 1.289    
0 2.140 2.151 2.146    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.; Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. In chronic inflammation, acts as a chemoattractant for neutrophils.; Acts as a chemoattractant for neutrophils in chronic inflammation.; adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in post-prandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-internalization and recycling of C5AR2.
Subcellular Location Secreted.
Database Links

KEGG: ssc:397072

STRING: 9823.ENSSSCP00000014399

UniGene: Ssc.61

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