Pig Pancreatic triacylglycerol lipase(PNLIP) ELISA kit

Pig Pancreatic triacylglycerol lipase(PNLIP) ELISA kit

Product Details

Customer Reviews and Q&A

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Product Details

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Customer Reviews and Q&A

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Pig Pancreatic triacylglycerol lipase(PNLIP) ELISA kit

Protocol MSDS

Code	CSB-EL018262PI
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Related Products
Customer Reviews and Q&A
Target Background

Target Name

pancreatic lipase

Alternative Names

PNLIPPancreatic triacylglycerol lipase ELISA Kit; PL ELISA Kit; PTL ELISA Kit; Pancreatic lipase ELISA Kit; EC 3.1.1.3 ELISA Kit

Abbreviation

PNLIP

Uniprot No.

P00591

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.625 mU/mL-40 mU/mL

Sensitivity

0.156 mU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig PNLIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	94
	Range %	86-100
1:2	Average %	95
	Range %	82-107
1:4	Average %	92
	Range %	81-103
1:8	Average %	96
	Range %	88-103

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml

OD1

OD2

Average

Corrected

2.052

2.137

2.095

2.007

1.639

1.658

1.649

1.561

1.110

1.134

1.122

1.034

0.753

0.711

0.732

0.644

2.5

0.405

0.392

0.399

0.311

1.25

0.312

0.303

0.308

0.220

0.625

0.189

0.181

0.185

0.097

0.086

0.090

0.088

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Pig PNLIP ELISA Kit was designed for the quantitative measurement of Pig PNLIP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.625 mU/mL-40 mU/mL and the sensitivity is 0.156 mU/mL .

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Function
(From Uniprot)

Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.

Subcellular Location

Secreted.

Protein Families

AB hydrolase superfamily, Lipase family

Database Links

STRING: 9823.ENSSSCP00000011355

UniGene: Ssc.61171

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ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.