Pig adiponectin,ADP ELISA Kit

Code CSB-E06839p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Pig ADP ELISA Kit was designed for the quantitative measurement of Pig ADP protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.875 μg/mL-30 μg/mL and the sensitivity is 1.875 μg/mL.

Target Name adiponectin,ADP
Abbreviation ADP
Uniprot No. Q6PP07
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.875 μg/mL-30 μg/mL
Sensitivity 1.875 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of pig ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 86-94  
1:2 Average % 96  
Range % 92-100  
1:4 Average % 83  
Range % 80-86  
1:8 Average % 91  
Range % 83-98  
The recovery of pig ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 87-99  
EDTA plasma (n=4) 93 91-96  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
30 0.153 0.165 0.159    
15 0.248 0.240 0.244    
7.5 0.534 0.511 0.523    
3.75 1.133 1.135 1.134    
1.875 2.340 2.260 2.300    
ELISA Data Analysis ELISA Standard Curve & Curve Expert software
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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