Pig interleukin 2,IL-2 ELISA Kit

Code CSB-E06783p
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 2
Alternative Names
IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
Abbreviation
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Sample

Serum(n=4)

1:1

Average %

104

Range %

97-111

1:2

Average %

90

Range %

85-96

1:4

Average %

95

Range %

88-101

1:8

Average %

89

Range %

82-96

Recovery

The recovery of pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

94

89-100

EDTA plasma (n=4)

90

85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

2.687

2.634

2.660

2.531

500

1.707

1.667

1.687

1.558

250

0.996

1.028

1.012

0.883

125

0.664

0.629

0.647

0.518

62.5

0.431

0.421

0.426

0.297

31.2

0.241

0.253

0.247

0.118

15.6

0.198

0.184

0.191

0.062

0

0.133

0.125

0.129

 

Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

Interleukin 2 (IL-2) is a cytokine that plays a central role in immune system regulation and T cell activation. Originally discovered as a T cell growth factor, IL-2 is primarily produced by activated CD4+ T helper cells and serves as a key mediator of adaptive immune responses. This cytokine promotes T cell proliferation, differentiation, and survival. It also influences the development and function of regulatory T cells. IL-2 signaling occurs through the IL-2 receptor complex and activates multiple downstream pathways that maintain immune homeostasis and mount effective immune responses against pathogens.

The Pig interleukin 2, IL-2 ELISA Kit (CSB-E06783p) is designed for quantitative measurement of IL-2 in Sus scrofa samples. This sandwich ELISA works with serum, plasma, and tissue homogenates with a detection range of 15.6 pg/mL to 1000 pg/mL and sensitivity of 3.9 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection is performed at 450 nm wavelength, providing researchers with a reliable tool for studying porcine IL-2 levels in various biological samples.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research examining immune responses and cytokine profiles in porcine models. The applications span studies of inflammatory responses following experimental infections and evaluations of immune system modulation in various physiological contexts.

Infection studies: Monitoring cytokine responses during experimental viral infections to characterize immune system activation and inflammatory cascades
Immune profiling: Quantifying cytokine levels in serum samples as part of comprehensive immune response evaluations alongside other inflammatory mediators
Tissue analysis: Measuring cytokine concentrations in tissue homogenates from liver, spleen, and intestinal mucosa to evaluate local immune responses
Temporal immune monitoring: Tracking cytokine levels across multiple time points to evaluate the progression and resolution of immune responses

Citations

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Target Background

Function
(From Uniprot)
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Gene References into Functions
  1. We observed that besides TCR stimulation Tregs require IL-2 for activation. PMID: 21530576
  2. a parameter of early mRNA expression during intestinal ischemia reperfusion injury PMID: 15050130
  3. The isolation and characterization of interleukin 2 from Tibetan swine are reported. PMID: 16195934
Subcellular Location
Secreted.
Protein Families
IL-2 family
Database Links
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