Pig interleukin 2,IL-2 ELISA Kit

Instructions
Code CSB-E06783p
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 2
Alternative Names IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
Abbreviation IL2
Uniprot No. P26891
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %80-99
1:2Average %86
Range %82-91
1:4Average %91
Range %86-97
1:8Average %94
Range %88-98
Recovery
The recovery of pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-102
EDTA plasma (n=4)9691-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.884 2.915 2.900 2.764
5002.123 2.104 2.114 1.978
2501.249 1.272 1.261 1.125
1250.818 0.846 0.832 0.696
62.50.492 0.440 0.466 0.330
31.20.314 0.322 0.318 0.182
15.60.263 0.258 0.261 0.125
00.135 0.136 0.136  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Gene References into Functions
  1. We observed that besides TCR stimulation Tregs require IL-2 for activation. PMID: 21530576
  2. a parameter of early mRNA expression during intestinal ischemia reperfusion injury PMID: 15050130
  3. The isolation and characterization of interleukin 2 from Tibetan swine are reported. PMID: 16195934
Subcellular Location Secreted
Protein Families IL-2 family
Database Links

KEGG: ssc:396868

STRING: 9823.ENSSSCP00000009697

UniGene: Ssc.16224

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