Pig luteinizing hormone (LH) ELISA kit

Instructions
Code CSB-E12870p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Alternative Names LHBLutropin subunit beta ELISA Kit; Lutropin beta chain ELISA Kit; Luteinizing hormone subunit beta ELISA Kit; LH-B ELISA Kit; LSH-B ELISA Kit; LSH-beta ELISA Kit
Abbreviation LH
Uniprot No. P01232
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 3 mIU/mL-600 mIU/mL
Sensitivity 1.5 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 84-98  
1:2 Average % 93  
Range % 89-99  
1:4 Average % 97  
Range % 92-103  
1:8 Average % 92  
Range % 83-97  
Recovery
The recovery of pig LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 91-103  
EDTA plasma (n=4) 98 93-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1 OD2 Average    
0 2.657 2.669 2.663    
3 1.452 1.507 1.480    
12 0.971 0.988 0.980    
48 0.500 0.516 0.508    
150 0.343 0.354 0.349    
600 0.152 0.153 0.153    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Gene References into Functions
  1. Changes in the expression of genes encoding beta-LH and GnRH-R were also demonstrated across the estrous cycle in swine PMID: 19212008
Subcellular Location Secreted
Protein Families Glycoprotein hormones subunit beta family
Database Links

KEGG: ssc:397153

STRING: 9823.ENSSSCP00000003414

UniGene: Ssc.15715

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