Rabbit Fibronectin(FN1) ELISA kit

Code CSB-EL008759RB
Size 96T,5×96T,10×96T
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Product Details

Target Name
fibronectin 1
Alternative Names
FN1Fibronectin ELISA Kit; FN ELISA Kit; Fragment ELISA Kit
Abbreviation
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.25 ng/mL-20 ng/mL
Sensitivity
1.25 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit FN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 95  
Range % 92-98  
1:200 Average % 103  
Range % 98-108  
1:400 Average % 98  
Range % 94-102  
1:800 Average % 92  
Range % 87-97  
Recovery
The recovery of rabbit FN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 96-101  
EDTA plasma (n=4) 89 82-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
20 0.150 0.160 0.155    
10 0.268 0.287 0.278    
5 0.552 0.566 0.559    
2.5 0.921 0.922 0.922    
1.25 1.477 1.424 1.451    
0 2.662 2.581 2.622    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit FN1 ELISA Kit was designed for the quantitative measurement of Rabbit FN1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.25 ng/mL-20 ng/mL and the sensitivity is 1.25 ng/mL.

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Target Background

Function
(From Uniprot)
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Tissue Specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
Database Links
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