Rabbit Pulmonary surfactant-associated protein B(SFTPB) ELISA kit

Code CSB-EL021173RB
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
surfactant protein B
Alternative Names
SFTPB ELISA Kit; SFTP3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; 6 kDa protein ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
Abbreviation
SFTPB
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.87 ng/mL-120 ng/mL
Sensitivity
0.46 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SFTPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %82-93
1:2Average %95
Range %91-99
1:4Average %94
Range %89-98
1:8Average %89
Range %86-96
Recovery
The recovery of rabbit SFTPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9286-98
EDTA plasma (n=4)8882-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1202.853 2.976 2.915 2.822
602.431 2.339 2.385 2.292
301.541 1.569 1.555 1.462
150.897 0.887 0.892 0.799
7.50.443 0.454 0.449 0.356
3.750.239 0.247 0.243 0.150
1.870.142 0.140 0.141 0.048
00.092 0.093 0.093
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit SFTPB ELISA Kit was designed for the quantitative measurement of Rabbit SFTPB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.87 ng/mL-120 ng/mL and the sensitivity is 0.46 ng/mL.

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Target Background

Function
(From Uniprot)
Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
Subcellular Location
Secreted, extracellular space, surface film.
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