Rabbit cholesteryl ester transfer protein,CETP ELISA Kit

Code CSB-E06841Rb
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
cholesteryl ester transfer protein, plasma
Alternative Names
CETPCholesteryl ester transfer protein ELISA kit; Lipid transfer protein I ELISA kit; Fragment ELISA kit
Abbreviation
CETP
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 ng/mL-100 ng/mL
Sensitivity
0.39 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CETP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 99  
Range % 94-102  
1:200 Average % 97  
Range % 94-100  
1:400 Average % 83  
Range % 80-86  
1:800 Average % 91  
Range % 87-95  
Recovery
The recovery of rabbit CETP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 100 97-103  
EDTA plasma (n=4) 84 81-89  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
100 0.082 0.086 0.084    
50 0.169 0.178 0.174    
25 0.241 0.258 0.250    
12.5 0.361 0.378 0.370    
6.25 0.582 0.541 0.562    
3.12 0.878 0.898 0.888    
1.56 1.208 1.231 1.220    
0 2.015 2.068 2.042    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit CETP ELISA Kit was designed for the quantitative measurement of Rabbit CETP protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

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Target Background

Function
(From Uniprot)
Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL. Regulates the reverse cholesterol transport, by which excess cholesterol is removed from peripheral tissues and returned to the liver for elimination.
Subcellular Location
Secreted.
Protein Families
BPI/LBP/Plunc superfamily, BPI/LBP family
Tissue Specificity
Expressed by the liver.
Database Links
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