Rat α2 macroglobulin,α2 MG ELISA Kit

Code CSB-E13863r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
alpha-2-macroglobulin
Alternative Names
A2m ELISA kit; A2m1Alpha-2-macroglobulin ELISA kit; Alpha-2-M ELISA kit
Abbreviation
A2M
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma
Detection Range
3.13 ng/mL-800 ng/mL
Sensitivity
3.125 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat α2 MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 97
Range % 88-105
1:2000 Average % 91
Range % 89-102
1:4000 Average % 93
Range % 90-98
1:8000 Average % 94
Range % 87-98
Recovery
The recovery of rat α2 MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 92-102
EDTA plasma (n=4) 95 90-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
800 0.218 0.214 0.216
200 0.451 0.461 0.456
50 0.850 0.861 0.856
12.5 1.225 1.242 1.234
3.125 1.726 1.737 1.732
0 1.944 1.967 1.956
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Citations

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Target Background

Function
(From Uniprot)
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Subcellular Location
Secreted.
Protein Families
Protease inhibitor I39 (alpha-2-macroglobulin) family
Tissue Specificity
Highest constitutive expression in ovary. Low level in testis, uterus and non-acute phase liver. Protein found in plasma.
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