Rat Apolipoprotein C-III(APOC3) ELISA kit

Code CSB-EL001933RA
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
apolipoprotein C-III
Alternative Names
Apoc3 ELISA Kit; Apolipoprotein C-III ELISA Kit; Apo-CIII ELISA Kit; ApoC-III ELISA Kit; Apolipoprotein C3 ELISA Kit
Abbreviation
APOC3
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 ng/mL-2000 ng/mL
Sensitivity
7.8 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat APOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 92
Range % 88-96
1:200 Average % 95
Range % 91-99
1:400 Average % 93
Range % 90-97
1:800 Average % 95
Range % 89-99
Recovery
The recovery of rat APOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 87-93
EDTA plasma (n=4) 87 84-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
2000 0.093 0.089 0.091
1000 0.152 0.144 0.148
500 0.227 0.218 0.223
250 0.336 0.328 0.332
125 0.498 0.488 0.493
62.5 0.657 0.640 0.649
31.25 0.963 0.893 0.928
0 1.673 1.583 1.628
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat APOC3 ELISA Kit was designed for the quantitative measurement of Rat APOC3 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 ng/mL-2000 ng/mL and the sensitivity is 7.8 ng/mL.

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Target Background

Function
(From Uniprot)
Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.
Subcellular Location
Secreted.
Protein Families
Apolipoprotein C3 family
Tissue Specificity
Synthesized predominantly in liver and to a lesser degree in intestine.
Database Links
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