Rat Cathepsin L1(CTSL1) ELISA kit

Code CSB-EL006193RA
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat CTSL1 ELISA Kit was designed for the quantitative measurement of Rat CTSL1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Target Name cathepsin L1
Alternative Names Ctsl ELISA Kit; Ctsl1Cathepsin L1 ELISA Kit; EC 3.4.22.15 ELISA Kit; Cathepsin L ELISA Kit; Cyclic protein 2 ELISA Kit; CP-2 ELISA Kit; Major excreted protein ELISA Kit; MEP) [Cleaved into: Procathepsin L; Cathepsin L1 heavy chain; Cathepsin L1 light chain] ELISA Kit
Abbreviation CTSL1
Uniprot No. P07154
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CTSL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 90-95
1:2 Average % 89
Range % 85-93
1:4 Average % 106
Range % 103-109
1:8 Average % 107
Range % 103-110
Recovery
The recovery of rat CTSL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 85 81-89
EDTA plasma (n=4) 87 83-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
400 1.916 1.843 1.880 1.698
200 1.452 1.465 1.459 1.277
100 0.925 0.932 0.929 0.747
50 0.572 0.581 0.577 0.395
25 0.406 0.436 0.421 0.239
12.5 0.305 0.300 0.303 0.121
6.25 0.256 0.249 0.253 0.071
0 0.181 0.183 0.182  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

Can you please tell me the host animal for the antibodies included in this kit?

A:
Thanks for your inquiry!
Biotin-antibody is detection antibody.
CSB-EL006193RA Capture antibody is mouse monoclonal antibody. Detection antibody is goat polyclonal antibody.
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
Thiol protease important for the overall degradation of proteins in lysosomes. Plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen. In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter. In thymus, regulates CD4(+) T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells. Also mediates invariant chain processing in cortical thymic epithelial cells. Major elastin-degrading enzyme at neutral pH. Accumulates as a mature and active enzyme in the extracellular space of antigen presenting cells (APCs) to regulate degradation of the extracellular matrix in the course of inflammation. Secreted form generates endostatin from COL18A1. Critical for cardiac morphology and function. Plays an important role in hair follicle morphogenesis and cycling, as well as epidermal differentiation. Required for maximal stimulation of steroidogenesis by TIMP1.
Gene References into Functions
  1. the activation of cathepsin L contributes to the irreversible depolarization produced by oxygen and glucose deprivation PMID: 27818353
  2. Cathepsins in Rotator Cuff Tendinopathy: Identification in Human Chronic Tears and Temporal Induction in a Rat Model. PMID: 25558848
  3. Inhibition of the activation of cathepsin L contributes to neuroprotection against cerebral ischemia. PMID: 24616078
  4. Changes in olfaction were less consistent and widespread in the hippocampus, but were again sex specific for three genes: cathepsin-L (CtsL), matrix metalloproteinase-14 (MMP-14) and MMP-16. PMID: 23103411
  5. With aging, the level of cathepsin L delined at 18 month but rose at 24 month in brain. PMID: 21374014
  6. double transgenic rats (human renin and angiotensinogen genes) were used to investigate sex differences influencing renal function and gene expression. Cathepsin L was differentially expressed between the sexes and associated with degree of renal injury. PMID: 21357272
  7. confirmed 6-hydroxydopamine induced nuclear translocation of cathepsin L in rat substantia nigral neurons as well PMID: 21134415
  8. study found, in differentiated PC12 cells, lysosomal membrane permeabilization is an early event in palmitic acid-induced lipotoxicity preceeding mitochondrial membrane permeabilization & apoptosis; cathepsin L is an important contributor in this process PMID: 20043885
  9. shows evolution in placental expression PMID: 12054558
  10. the GC-box is a critical regulatory element for the cathepsin L promoter in mature Sertoli cells PMID: 12606333
  11. data document that three Sp1/specificity protein 3 binding regions and a functional cyclic amp regulatory element constitute an important transcriptional regulatory complex for expression of the cathepsin L gene in rat granulosa cells PMID: 14563703
  12. The induction of puromycin aminonucleoside nephrosis involves podocyte migration conducted by a coordinated interplay between the cysteine protease cathepsin L and alpha(3) integrin PMID: 15197181
  13. endothelial cell-associated cathepsins B and L are not involved in the invasive growth of capillaries from existing blood vessels and the presence of collagen is necessary for MMP2 expression in endothelial cells PMID: 15255544
  14. The co-localization of PAP/TRAP and the cysteine protease cathepsin L could suggest a role for cathepsin L in the in vivo proteolytic processing of PAP/TRAP. PMID: 15761664
  15. Results show Cathepsin L plays a critical role in the lysosomal degradation of L-lactate dehydrogenase. PMID: 16960372
  16. Our data strongly suggests that the decrease in heat shock protein levels and the significant reduction of serum albumin leakage into the brain following acute treatment with CP-1 is indicative of less secondary ischemic damage. PMID: 18060871
  17. Study indicates that CTSL improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking Akt/GSK3beta signaling. PMID: 19096818
  18. Upstream repressors and activators as well as cis-acting elements near the transcription start site control stage-specific Ctsl transcription by Sertoli cells. PMID: 19458314

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Subcellular Location Lysosome. Apical cell membrane; Peripheral membrane protein; Extracellular side. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Secreted, extracellular space. Secreted.
Protein Families Peptidase C1 family
Tissue Specificity Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII.
Database Links

KEGG: rno:25697

STRING: 10116.ENSRNOP00000025462

UniGene: Rn.1294

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