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| Code | CSB-E13416r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat COX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:400 | Average % | 93 | ||||
| Range % | 85-99 | |||||
| 1:800 | Average % | 101 | ||||
| Range % | 95-107 | |||||
| The recovery of rat COX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 90 | 84-94 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This rat cyclooxygenase-1 (COX-1) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat COX-1 in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of COX-1 bound in the initial step.
COX-1 (PTGS1) is a constitutive enzyme expressed in the vascular endothelium, in platelets, and in many other cells in the body. It is responsible for the initial cyclooxygenase reaction, the target for NSAIDs, the conversion of arachidonic acid to PGG2, and the subsequent peroxidase reaction that converts PGG2 to PGH2. Several studies suggest that COX-1 also plays a role in neuroinflammation. COX-1 also mediates prostaglandin-dependent gastric protection. In gastric mucosa, COX-1 is involved in the synthesis of PGE2 and PGI2, which exert cytoprotective effects on several aspects of gastric function such as an increase of bicarbonate and mucus secretion, reduction of gastric acid and pepsin secretion, and maintaining adequate blood flow to the mucosa.
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STRING: 10116.ENSRNOP00000010218
UniGene: Rn.44404
