Rat Cyclooxygenase-1(COX-1) ELISA Kit

Instructions
Code CSB-E13416r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
Alternative Names Ptgs1 ELISA Kit; Cox-1 ELISA Kit; Cox1 ELISA Kit; Prostaglandin G/H synthase 1 ELISA Kit; EC 1.14.99.1 ELISA Kit; Cyclooxygenase-1 ELISA Kit; COX-1 ELISA Kit; Prostaglandin H2 synthase 1 ELISA Kit; PGH synthase 1 ELISA Kit; PGHS-1 ELISA Kit; PHS 1 ELISA Kit; Prostaglandin-endoperoxide synthase 1 ELISA Kit
Abbreviation PTGS1
Uniprot No. Q63921
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat COX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 86  
Range % 80-92  
1:200 Average % 92  
Range % 88-97  
1:400 Average % 93  
Range % 85-99  
1:800 Average % 101  
Range % 95-107  
Recovery
The recovery of rat COX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 90 84-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 2.448 2.352 2.400 2.262  
250 2.035 1.989 2.012 1.874  
125 1.512 1.501 1.507 1.369  
62.5 0.922 0.879 0.901 0.763  
31.2 0.491 0.473 0.482 0.344  
15.6 0.336 0.318 0.327 0.189  
7.8 0.207 0.199 0.203 0.065  
0 0.139 0.137 0.138    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.
Subcellular Location Microsome membrane, Peripheral membrane protein, Endoplasmic reticulum membrane, Peripheral membrane protein
Protein Families Prostaglandin G/H synthase family
Database Links

STRING: 10116.ENSRNOP00000010218

UniGene: Rn.44404

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