Target Name |
prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) |
Alternative Names |
Ptgs1 ELISA Kit; Cox-1 ELISA Kit; Cox1 ELISA Kit; Prostaglandin G/H synthase 1 ELISA Kit; EC 1.14.99.1 ELISA Kit; Cyclooxygenase-1 ELISA Kit; COX-1 ELISA Kit; Prostaglandin H2 synthase 1 ELISA Kit; PGH synthase 1 ELISA Kit; PGHS-1 ELISA Kit; PHS 1 ELISA Kit; Prostaglandin-endoperoxide synthase 1 ELISA Kit |
Abbreviation |
PTGS1 |
Uniprot No. |
Q63921 |
Species |
Rattus norvegicus (Rat) |
Sample Types |
serum, plasma, tissue homogenates |
Detection Range |
7.8 pg/mL-500 pg/mL |
Sensitivity |
1.95 pg/mL |
Assay Time |
1-5h |
Sample Volume |
50-100ul |
Detection Wavelength |
450 nm |
Research Area |
Metabolism
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Assay Principle |
quantitative |
Measurement |
Sandwich |
Precision |
Intra-assay Precision (Precision within an assay): CV%<8% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<10% |
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Three samples of known concentration were tested in twenty assays to assess. |
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Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of rat COX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
Sample |
Serum(n=4) |
|
1:100 |
Average % |
86 |
|
Range % |
80-92 |
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1:200 |
Average % |
92 |
|
Range % |
88-97 |
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1:400 |
Average % |
93 |
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Range % |
85-99 |
|
1:800 |
Average % |
101 |
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Range % |
95-107 |
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Recovery |
The recovery of rat COX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type |
Average % Recovery |
Range |
|
Serum (n=5) |
95 |
89-98 |
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EDTA plasma (n=4) |
90 |
84-94 |
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Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
500 |
2.448 |
2.352 |
2.400 |
2.262 |
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250 |
2.035 |
1.989 |
2.012 |
1.874 |
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125 |
1.512 |
1.501 |
1.507 |
1.369 |
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62.5 |
0.922 |
0.879 |
0.901 |
0.763 |
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31.2 |
0.491 |
0.473 |
0.482 |
0.344 |
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15.6 |
0.336 |
0.318 |
0.327 |
0.189 |
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7.8 |
0.207 |
0.199 |
0.203 |
0.065 |
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0 |
0.139 |
0.137 |
0.138 |
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Troubleshooting
and FAQs |
ELISA kit FAQs |
Storage |
Store at 2-8°C. Please refer to protocol. |
Lead Time |
3-5 working days |