Rat Cyclooxygenase-1(COX-1) ELISA Kit

Product Details

Citations

Target Data

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Rat Cyclooxygenase-1(COX-1) ELISA Kit

Name: Rat Cyclooxygenase-1(COX-1) ELISA Kit
Brand: CUSABIO
SKU: CSB-E13416r

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Code	CSB-E13416r
Size	96T,5×96T,10×96T
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Product Details
Citations
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Target Data

Target Name

prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)

Alternative Names

Ptgs1 ELISA Kit; Cox-1 ELISA Kit; Cox1 ELISA Kit; Prostaglandin G/H synthase 1 ELISA Kit; EC 1.14.99.1 ELISA Kit; Cyclooxygenase-1 ELISA Kit; COX-1 ELISA Kit; Prostaglandin H2 synthase 1 ELISA Kit; PGH synthase 1 ELISA Kit; PGHS-1 ELISA Kit; PHS 1 ELISA Kit; Prostaglandin-endoperoxide synthase 1 ELISA Kit

Abbreviation

PTGS1

Uniprot No.

Q63921

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.8 pg/mL-500 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat COX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	80-92
1:200	Average %	92
	Range %	88-97
1:400	Average %	93
	Range %	85-99
1:800	Average %	101
	Range %	95-107

Recovery

The recovery of rat COX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	90	84-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.448	2.352	2.400	2.262
250	2.035	1.989	2.012	1.874
125	1.512	1.501	1.507	1.369
62.5	0.922	0.879	0.901	0.763
31.2	0.491	0.473	0.482	0.344
15.6	0.336	0.318	0.327	0.189
7.8	0.207	0.199	0.203	0.065
0	0.139	0.137	0.138

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Phenylethanol Glycosides Protect Myocardial Hypertrophy Induced by Abdominal Aortic Constriction via ECE-1 Demethylation Inhibition and PI3K/PKB/eNOS Pathway Enhancement QL Fan,Evidence-Based Complementary and Alternative Medicine,2020
Carvacrol mitigates proconvulsive effects of lipopolysaccharide, possibly through the hippocampal cyclooxygenase-2 inhibition Mehdi Sadegh.et al,Metabolic Brain Disease,2018

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function	Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.
Subcellular Location	Microsome membrane, Peripheral membrane protein, Endoplasmic reticulum membrane, Peripheral membrane protein
Protein Families	Prostaglandin G/H synthase family
Database Links	STRING: 10116.ENSRNOP00000010218 UniGene: Rn.44404