Free triiodothyronine (FT3) is the unbound, metabolically active form of the thyroid hormone triiodothyronine that circulates freely in the bloodstream. FT3 is the most potent thyroid hormone and regulates cellular metabolism, protein synthesis, and energy production throughout the body. Unlike total T3 measurements, FT3 levels provide a more accurate picture of thyroid function since thyroid-binding protein variations don't affect them. FT3 measurements are important in clinical and research settings for evaluating thyroid disorders, metabolic studies, and understanding hormonal regulation mechanisms.
The Rat Free Tri-iodothyronine ELISA Kit (CSB-E05076r) is designed for quantitative measurement of FT3 in rat samples using a competitive assay principle. This kit works with multiple sample types including serum, plasma, tissue homogenates, and cell lysates with a sample volume requirement of 50-100 μL. The assay provides a detection range of 2-32 pmol/L with a sensitivity of 0.38 pmol/L, using detection at 450 nm wavelength. The complete assay can be performed within 1-5 hours, making it suitable for efficient laboratory workflows.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research examining thyroid hormone regulation and endocrine function in rat models. Studies have applied the kit to measure free triiodothyronine levels in serum samples as part of comprehensive thyroid profiling work. The kit supports research examining hormonal responses in various experimental contexts and therapeutic interventions.
• Thyroid function studies: Measurement of free triiodothyronine levels alongside other thyroid hormones in serum samples to evaluate overall thyroid profile and endocrine status
• Traditional medicine research: Examining herbal compound effects on thyroid hormone levels in experimental rat models
• Endocrine profiling: Comprehensive hormonal studies measuring multiple thyroid-related parameters in serum samples
• Biochemical parameter analysis: Integration with broader metabolic and inflammatory marker work in research examining multiple physiological systems
