Rat Keratinocyte Growth Factor,KGF ELISA Kit

Code CSB-E12905r
Size 96T,5×96T,10×96T
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Product Details

Target Name
fibroblast growth factor 7 (keratinocyte growth factor)
Alternative Names
Fgf7 ELISA Kit; Fgf-7 ELISA Kit; KgfFibroblast growth factor 7 ELISA Kit; FGF-7 ELISA Kit; Heparin-binding growth factor 7 ELISA Kit; HBGF-7 ELISA Kit; Keratinocyte growth factor ELISA Kit
Abbreviation
FGF7
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %86
Range %81-95
1:2Average %95
Range %89-101
1:4Average %92
Range %87-96
1:8Average %86
Range %80-93
Recovery
The recovery of rat KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10094-105
EDTA plasma (n=4)9589-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.730 2.775 2.753 2.621
10002.226 2.403 2.315 2.183
5001.500 1.610 1.555 1.423
2500.837 0.890 0.864 0.732
1250.428 0.432 0.430 0.298
62.50.221 0.235 0.228 0.096
31.20.167 0.158 0.163 0.031
00.130 0.133 0.132  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat FGF7 ELISA Kit was designed for the quantitative measurement of Rat FGF7 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .

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Target Background

Function
(From Uniprot)
Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
Gene References into Functions
  1. The findings of the present study indicated that KGF, CTGF and TGF-beta have biological roles in progression of CsA- and PNT- induced GO. KGF plays a greater role in CsA- induced GO than in PNT- induced GO. Meanwhile, CTGF and TGF-beta play a role in PNT- induced GO greater than in CsA- induced GO. PMID: 26894526
  2. These data demonstrate that administration of MSCs to the inflammation-insulted AT-II cells may ameliorate the impairments through a KGF-dependent PI3K/AKT/mTOR signaling pathway. PMID: 27035760
  3. The primary role of both KGF1 and FGF2 appeared to be in the proliferation and migration of keratinocytes PMID: 21136143
  4. KGF may have a role in ameliorating radiation-induced pulmonary injury in rats PMID: 21436609
  5. KGF promotes preadipocyte proliferation by an autocrine mechanism that involves activation of the PI3K/Akt signaling pathway PMID: 20069574
  6. Keratinocyte growth factor may promote alveolar epithelial repair through several mechanisms involved in cell migration PMID: 12581741
  7. Decreased gene expression of FGF-7 in the rat hypoplastic lung suggests that pulmonary hypoplasia in nitrofen-induced congenital diaphragmatic hernia rat may be caused by reduced synthesis of FGF-7 during lung morphogenesis. PMID: 12861530
  8. KGF stimulates lipogenesis in alveolar type II cells by a coordinated expression of lipogenic enzymes and transport proteins regulated by C/EBP isoforms and SREBP-1c PMID: 12865412
  9. stimulates migration and hyaluronan synthesis in the epidermis by activation of keratinocyte hyaluronan synthases 2 and 3 PMID: 14506240
  10. Exogenous administered KGF cDNA causes increases in IGF-I, IGF-BP3, FGF, and collagen IV and decreases TGF-beta concentration. KGF gene transfer accelerates wound healing without causing an increase in collagen I or III. PMID: 17292422
  11. KGF improves alterations of lung permeability and bronchial epithelium in allergic rats PMID: 17392324
  12. retention of the AT2 cell phenotype by KGF involves c-Jun and suggest that activation of c-Jun kinase may be an important determinant of maintenance of AT2 cell phenotype PMID: 17872496
  13. FGF-7, most likely through its effect on the vascular bed, is required for normal early postnatal alveolar formation from secondary crests. PMID: 18091341
  14. the effects of KGF on duct cell proliferation are mediated by the MEK-ERK1/2 pathway, while the KGF-induced cell differentiation is mediated by the PI3K/AKT pathway PMID: 19266047

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Protein Families
Heparin-binding growth factors family
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