Rat L-Lactate Dehydrogenase,L-LDH ELISA Kit

Code CSB-E11324r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
lactate dehydrogenase A
Alternative Names
Ldha ELISA Kit; Ldh-1 ELISA Kit; Ldh1 ELISA Kit; L-lactate dehydrogenase A chain ELISA Kit; LDH-A ELISA Kit; EC 1.1.1.27 ELISA Kit; LDH muscle subunit ELISA Kit; LDH-M ELISA Kit
Abbreviation
LDHA
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
39 mU/mL-2500 mU/mL
Sensitivity
9.77 mU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat L-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %80-97
1:2Average %92
Range %88-105
1:4Average %97
Range %92-104
1:8Average %94
Range %86-98
Recovery
The recovery of rat L-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-100
EDTA plasma (n=4)9085-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
25002.754 2.952 2.853 2.679
12502.218 2.376 2.297 2.123
6251.534 1.584 1.559 1.385
312.51.022 1.039 1.031 0.857
1560.587 0.624 0.606 0.432
780.412 0.430 0.421 0.247
390.286 0.279 0.283 0.109
00.173 0.175 0.174  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The rat LDHA ELISA Kit is engineered for accurate measurement of rat LDHA levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the LDHA content in the sample. The color intensity is positively correlated with LDHA content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

LDHA is a cytosolic enzyme that catalyzes the conversion of pyruvate to lactate under anaerobic conditions. It is primarily found in skeletal muscle. LDHA is necessary to maintain glycolysis and ATP synthesis in the absence of sufficient oxygen by recycling NAD+ from NADH. LDHA also participates in the modulation of transcription by regulating the cellular redox state. During the differentiation of thymocytes, LDHA also functions as a molecular chaperone or as an association molecule. Even when oxygen is available, cancer cells employ LDHA to boost glycolysis, ATP, and lactate synthesis. Overexpression of LDHA has been associated with a number of additional unfavorable prognostic variables, including tumor hypoxia, angiogenesis, proliferation, and glucose uptake, as well as chemotherapy and radiotherapy resistance.

Citations

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Gene References into Functions
  1. LDHA is a direct target of miR-34a, which binds to the 3'-UTR region of LDHA mRNA in cardiomyocytes. PMID: 28894025
  2. role of lactate dehydrogenase-A (LDHA), which is a key enzyme for glycolysis, in the proliferation and migration of vascular smooth muscle cells PMID: 28818664
  3. After 30 min of ischemia, the kidney maintains LDH metabolic function in spite of decreased kidney function, whereas the postischemic kidney after 60 min of ischemia show a generally reduced LDH enzyme activity concomitant with a reduced kidney function. PMID: 27654895
  4. The expression of three well-defined downstream targets of hypoxia inducible factor 1 alpha subunit (HIF-1alpha), pyruvate dehydrogenase kinase 1 (PDK1), lactate dehydrogenase A (LDHA) and vascular endothelial growth factor (VEGF), were up-regulated in hippocampus after 28 days of simulated microgravity exposure. PMID: 27988334
  5. aging alters aspects of skeletal muscle isoform shift induced by overload and anabolic steroid administration PMID: 24835193
  6. Lactate dehydrogenase a expression is necessary to sustain rapid angiogenesis of pulmonary microvascular endothelium. PMID: 24086675
  7. lactate dehydrogenase subunit A mRNA stability is regulated by Cyclic AMP and AKAP-mediated targeting of protein kinase A PMID: 15878851
  8. M-LDH released from cardiomyocytes after hypoxia and reoxygenation has a role in protecting the heart from oxidative stress-induced injury through an intracellular signal transduction pathway involving ERK1/2. PMID: 18534967

Show More

Hide All

Subcellular Location
Cytoplasm.
Protein Families
LDH/MDH superfamily, LDH family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1