Rat L-Lactate Dehydrogenase,L-LDH ELISA Kit

Instructions
Code CSB-E11324r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name lactate dehydrogenase A
Alternative Names Ldha ELISA Kit; Ldh-1 ELISA Kit; Ldh1 ELISA Kit; L-lactate dehydrogenase A chain ELISA Kit; LDH-A ELISA Kit; EC 1.1.1.27 ELISA Kit; LDH muscle subunit ELISA Kit; LDH-M ELISA Kit
Abbreviation LDHA
Uniprot No. P04642
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 39 mU/mL-2500 mU/mL
Sensitivity 9.77 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat L-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %80-97
1:2Average %92
Range %88-105
1:4Average %97
Range %92-104
1:8Average %94
Range %86-98
Recovery
The recovery of rat L-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-100
EDTA plasma (n=4)9085-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
25002.754 2.952 2.853 2.679
12502.218 2.376 2.297 2.123
6251.534 1.584 1.559 1.385
312.51.022 1.039 1.031 0.857
1560.587 0.624 0.606 0.432
780.412 0.430 0.421 0.247
390.286 0.279 0.283 0.109
00.173 0.175 0.174  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Gene References into Functions
  1. LDHA is a direct target of miR-34a, which binds to the 3'-UTR region of LDHA mRNA in cardiomyocytes. PMID: 28894025
  2. role of lactate dehydrogenase-A (LDHA), which is a key enzyme for glycolysis, in the proliferation and migration of vascular smooth muscle cells PMID: 28818664
  3. After 30 min of ischemia, the kidney maintains LDH metabolic function in spite of decreased kidney function, whereas the postischemic kidney after 60 min of ischemia show a generally reduced LDH enzyme activity concomitant with a reduced kidney function. PMID: 27654895
  4. The expression of three well-defined downstream targets of hypoxia inducible factor 1 alpha subunit (HIF-1alpha), pyruvate dehydrogenase kinase 1 (PDK1), lactate dehydrogenase A (LDHA) and vascular endothelial growth factor (VEGF), were up-regulated in hippocampus after 28 days of simulated microgravity exposure. PMID: 27988334
  5. aging alters aspects of skeletal muscle isoform shift induced by overload and anabolic steroid administration PMID: 24835193
  6. Lactate dehydrogenase a expression is necessary to sustain rapid angiogenesis of pulmonary microvascular endothelium. PMID: 24086675
  7. lactate dehydrogenase subunit A mRNA stability is regulated by Cyclic AMP and AKAP-mediated targeting of protein kinase A PMID: 15878851
  8. M-LDH released from cardiomyocytes after hypoxia and reoxygenation has a role in protecting the heart from oxidative stress-induced injury through an intracellular signal transduction pathway involving ERK1/2. PMID: 18534967

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Subcellular Location Cytoplasm
Protein Families LDH/MDH superfamily, LDH family
Database Links

KEGG: rno:24533

STRING: 10116.ENSRNOP00000017468

UniGene: Rn.107896

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