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| Code | CSB-E11324r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat L-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 80-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
| The recovery of rat L-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-100 | ||||
| EDTA plasma (n=4) | 90 | 85-95 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The rat LDHA ELISA Kit is engineered for accurate measurement of rat LDHA levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the LDHA content in the sample. The color intensity is positively correlated with LDHA content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.
LDHA is a cytosolic enzyme that catalyzes the conversion of pyruvate to lactate under anaerobic conditions. It is primarily found in skeletal muscle. LDHA is necessary to maintain glycolysis and ATP synthesis in the absence of sufficient oxygen by recycling NAD+ from NADH. LDHA also participates in the modulation of transcription by regulating the cellular redox state. During the differentiation of thymocytes, LDHA also functions as a molecular chaperone or as an association molecule. Even when oxygen is available, cancer cells employ LDHA to boost glycolysis, ATP, and lactate synthesis. Overexpression of LDHA has been associated with a number of additional unfavorable prognostic variables, including tumor hypoxia, angiogenesis, proliferation, and glucose uptake, as well as chemotherapy and radiotherapy resistance.
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