Rat Lecithin Cholesterol Acyltransferase(LCAT) ELISA Kit

Instructions
Code CSB-E14201r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name lecithin-cholesterol acyltransferase
Alternative Names LcatPhosphatidylcholine-sterol acyltransferase ELISA kit; EC 2.3.1.43 ELISA kit; Lecithin-cholesterol acyltransferase ELISA kit; Phospholipid-cholesterol acyltransferase ELISA kit
Abbreviation LCAT
Uniprot No. P18424
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.87 ng/mL-120 ng/mL
Sensitivity 0.94 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LCAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %102
Range %92-107
1:200Average %88
Range %84-93
1:400Average %101
Range %94-105
1:800Average %102
Range %92-105
Recovery
The recovery of rat LCAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-96
EDTA plasma (n=4)10292-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
1200.075 0.079 0.077
600.172 0.178 0.175
300.374 0.382 0.378
150.634 0.624 0.629
7.51.178 1.146 1.162
3.751.504 1.533 1.519
1.871.673 1.622 1.648
02.155 2.078 2.117
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (By similarity). Has a preference for plasma 16
Subcellular Location Secreted
Protein Families AB hydrolase superfamily, Lipase family
Tissue Specificity Detected in blood plasma (at protein level) (PubMed:8820107).
Database Links

STRING: 10116.ENSRNOP00000026583

UniGene: Rn.10481

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