Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit

Code CSB-EL021339RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Alternative Names RP11-57G10.3, SIR2L1, SIR2alpha|sir2-like 1|sirtuin 1|sirtuin type 1
Abbreviation SIRT1
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 34.3 pg/mL-2200 pg/mL
Sensitivity 8.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat SIRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %92
Range %86-94
1:2Average %102
Range %97-105
1:4Average %91
Range %87-95
1:8Average %98
Range %92-102
The recovery of rat SIRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)9995-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
22002.357 2.272 2.315 2.209
11001.976 1.873 1.925 1.819
5501.582 1.486 1.534 1.428
2751.141 1.158 1.150 1.044
137.50.761 0.757 0.759 0.653
68.750.436 0.425 0.431 0.325
34.30.262 0.266 0.264 0.158
00.107 0.105 0.106
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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