Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
| Code | CSB-EL021339RA |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SIRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-105 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 87-95 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-102 | |||||
| The recovery of rat SIRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 99 | 95-105 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit is the ideal tool for researchers working in the field of epigenetics and nuclear signaling. This quantitative sandwich assay is designed to detect and quantify the protein expression levels of NAD-dependent protein deacetylase sirtuin-1, also known as SIRT1, in serum, plasma, tissue homogenates, and cell lysates from Rattus norvegicus (Rat).
With a detection range of 34.3 pg/mL-2200 pg/mL and a sensitivity of 8.5 pg/mL, this ELISA kit provides accurate and precise results for your research needs. The assay time is between 1-5 hours, and the required sample volume is only 50-100ul, making it a convenient and efficient tool for your laboratory.
The detection wavelength of 450 nm ensures reliable and consistent results, and the kit comes with all the necessary components and reagents to perform the assay. With more than 3 citations, this kit has been validated and tested by other researchers, providing you with confidence in your results.
There are currently no reviews for this product.
