Mouse NAD-dependent deacetylase sirtuin-1(Sirt1)ELISA Kit

Instructions
Code CSB-E16187m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Alternative Names Sirt1 ELISA Kit; Sir2l1 ELISA Kit; NAD-dependent protein deacetylase sirtuin-1 ELISA Kit; EC 2.3.1.286 ELISA Kit; NAD-dependent protein deacylase sirtuin-1 ELISA Kit; EC 2.3.1.- ELISA Kit; Regulatory protein SIR2 homolog 1 ELISA Kit; SIR2-like protein 1 ELISA Kit; SIR2alpha ELISA Kit; Sir2 ELISA Kit; mSIR2a) [Cleaved into: SirtT1 75 kDa fragment ELISA Kit; 75SirT1)] ELISA Kit
Abbreviation SIRT1
Uniprot No. Q923E4
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Sirt1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 85-95  
1:2 Average % 93  
Range % 88-98  
1:4 Average % 95  
Range % 89-100  
1:8 Average % 97  
Range % 93-103  
Recovery
The recovery of mouse Sirt1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 95-104  
EDTA plasma (n=4) 98 92-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.322 2.162 2.242 2.071  
5 1.516 1.447 1.482 1.311  
2.5 0.897 0.834 0.866 0.695  
1.25 0.512 0.509 0.511 0.340  
0.625 0.349 0.327 0.338 0.167  
0.312 0.278 0.258 0.268 0.097  
0.156 0.232 0.219 0.226 0.055  
0 0.175 0.167 0.171    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell
Gene References into Functions
  1. Study found little evidence that Sirt1 promotes the development of food anticipatory activity (FAA) and no evidence for the involvement of Sirt1 in the maintenance of FAA; nor much effect on body weight homeostasis during 60% timed calorie-restricted (CR) feeding over a short-term CR experiment. PMID: 29940007
  2. Authors report here that SIRT1 levels were decreased in the liver in different mouse models and in cultured HSCs undergoing activation. PMID: 28919365
  3. Authors concluded that miR-29b is an important regulator in the PDGF-BB-mediated VSMC phenotypic transition by targeting SIRT1. PMID: 30231015
  4. In the absence of Sirt1, both embryos and placentas were small, with placentas showing abnormalities in both the labyrinthine layer and junctional zone. Sirt1-null trophoblastic stem cells showed blunted differentiation, and appeared to be suspended in an Epcam(high) trophoblast progenitor state. PMID: 29405961
  5. the Sirt1 carboxyl-domain is an ATP-repressible domain that is transferrable to other proteins PMID: 28504272
  6. The present study has provided novel evidence to suggest that under HFD-induced metabolic surplus, the lack of SIRT1 catalytic activity promotes release of free fatty acid from mesenteric adipose tissue (MAT) and escalate nonalcoholic fatty liver disease (NAFLD) by interfering with lipid homeostasis in both liver and MAT. PMID: 28789977
  7. Compared with control-diet mice, mice given an high-fat diet (HFD) for 4weeks displayed anxiolytic-like behaviors. At the same time, we observed decreased SIRT1 expression in the medial prefrontal cortex and the amygdala of HFD-fed mice. Resveratrol, an activator of SIRT1, reversed the anxiolytic-like behaviors in HFD-fed mice. PMID: 29331532
  8. These findings support the unifying concept that nuclear NAD(+) sensor SIRT1 broadly coordinates innate and adaptive immune reprogramming during sepsis PMID: 30069485
  9. Low SIRT1 expression is associated with Parkinson's disease. PMID: 29571747
  10. SUV39H augments intracellular reactive oxygen species levels in a SIRT1-dependent manner. PMID: 28361889
  11. our data strongly indicate that melatonin delays postovulatory mouse oocyte aging via a SIRT1-MnSOD-dependent pathway PMID: 29752296
  12. SIRT1 upregulation protects against liver injury induced by a HFD through inhibiting CD36 and the NF-kappaB pathway in mouse Kupffer cells. PMID: 29845302
  13. Low expression of SIRT1 is associated with Human immunodeficiency virus-associated nephropathy. PMID: 29608911
  14. SIRT1 levels were down-regulated in the livers in models of liver fibrosis and in activated hepatic stellate cells (HSCs) as opposed to quiescent HSCs. SIRT1 activation halted, whereas SIRT1 inhibition promoted, HSC transdifferentiation into myofibroblasts. PMID: 28970250
  15. Sirt1 preserves Cav1-dependent endothelial function by mitigating miR-204-mediated vascular endoplasmic reticulum stress. PMID: 28181559
  16. Nicotinamide riboside (NR) can protect against ethanol induced liver injuries via replenishing NAD(+), reducing oxidative stress, and activating SirT1-PGC-1a-mitochondrial biosynthesis. Our data indicate that SirT1 plays an important role in the protection of NR against lipid accumulation and mitochondrial dysfunctions induced by ethanol PMID: 29679894
  17. These data highlight the importance of preventing the loss of 24-OH in the brain, and of maintaining high levels of the enzyme SIRT1, in order to counteract neurodegeneration. PMID: 29883958
  18. the results of the present study indicated that GLP1 may be a promising target for the development of novel therapeutic strategies for HGinduced nephropathy, and may function through the activation of SIRT1 PMID: 29845208
  19. In lung tissues of LPS-endotoxemia, significantly increased levels of SNO-SIRT were found. Plasma nitrite and HMGB1 levels were significantly higher than those in the sham controls. PMID: 29680657
  20. targeted induction of the anti-inflammatory effects of SIRT1 and LCN2 may help prevent obesity-associated insulin resistance and neuroinflammation. PMID: 29634925
  21. PE particle-induced downregulation of SIRT1 enhances ER stress. PMID: 29775898
  22. During catecholamine-stimulated lipolysis, Perilipin 5 is phosphorylated by protein kinase A and forms transcriptional complexes with PGC-1alpha and SIRT1 in the nucleus. Perilipin 5 promotes PGC-1alpha co-activator function by disinhibiting SIRT1 deacetylase activity. PMID: 27554864
  23. findings demonstrate a new mechanism for the activation of SIRT1 under stress conditions and suggest a novel potential therapeutic target for preventing age-related diseases and extending healthspan PMID: 29133780
  24. SIRT1 was inhibited or knocked out in macrophages, the protection effect disappeared by treatment of inhibitor of mir-138, and the NFkappaB pathway was activated and the AKT pathway was inhibited PMID: 30157481
  25. the results of the present study suggest that moderate overexpression of SIRT1 (~3fold of normal level) may directly or indirectly inhibit apoptosis of OBs via the FOXO1 and betacatenin signaling pathway. PMID: 29512706
  26. Overexpression of miR-22 can attenuate oxidative stress injury in diabetic cardiomyopathy by upregulation of Sirt 1. PMID: 29288528
  27. caloric restriction rescues SIRT1 levels in transgenic Machado-Joseph disease (MJD) mice, whereas silencing SIRT1 is sufficient to prevent the beneficial effects on MJD pathology. PMID: 27165717
  28. the findings identify a gut-vascular axis in which Gut microbiota remotely upregulate miR-204 expression in the vessel wall. Among the genes targeted by miR-204 in the vascular wall is Sirt1, downregulation of which promotes endothelial dysfunction. PMID: 27586459
  29. melatonin oral supplementation may represent a new protective approach to block hypercholesterolemic liver alterations involving also a SIRT1-dependent mechanism. PMID: 29516009
  30. Melatonin promoted MnSOD and SIRT1 expression, which successfully ameliorated busulfan-induced SSC apoptosis caused by high concentrations of ROS and p53 PMID: 28027652
  31. HO-1 regulates macrophage activation via the SIRT1-p53 signaling network and regulates hepatocellular death in liver ischemia-reperfusion injury. PMID: 28842295
  32. miR-200a-3p promotes beta-amyloid-induced neuronal apoptosis through down-regulation of SIRT1 in Alzheimer's disease and its animal models. PMID: 29358553
  33. The nicotinamide adenine dinucleotide (NAD)-dependent deacetylase SIRT1 acts as an energy sensor and negatively regulates poly(A)RNA transport via deacetylating a poly(A)-binding protein, PABP1. PMID: 28756945
  34. L-Carnitine alleviated epithelial mesenchymal transformation-associated renal fibrosis caused by perfluorooctanesulfonate through a Sirt1- and PPARgamma-dependent mechanism. PMID: 28973641
  35. The data, therefore, suggest that 1,25(OH)2D3 increases glucose consumption by inducing SIRT1 activation, which in turn increases IRS1 phosphorylation and GLUT4 translocation in myotubes. PMID: 29188534
  36. PU.1 suppresses Sirt1 translation via transcriptional promotion of miR-34a/-29c. PMID: 29162670
  37. Impairment of energy sensors, SIRT1 and AMPK, in lipid induced inflamed adipocyte is regulated by Fetuin A. PMID: 29030114
  38. this study revealed a new mechanism wherein EPO alleviates hepatic steatosis by activating autophagy via SIRT1-dependent deacetylation of LC3. PMID: 29522896
  39. Retinoic acid (RA) increases the expression of hepatic Sirt1 and inhibits the expression of sterol regulatory element binding protein 1c (Srebp-1c) in WT mice in vivo and in vitro. RA-mediated molecular effects are abolished in the liver and primary hepatocytes from Sirtuin 1 (Sirt1) deficient (LKO) mice. PMID: 28667519
  40. thymoquinone inhibits cellular ROS generation in LPS-activated BV2 microglia. It is also suggested that activation of both AMPK and NAD(+)/SIRT1 may contribute to the anti-inflammatory, but not antioxidant activity of the compound in BV2 microglia. PMID: 28551846
  41. SIRT1 signaling is important for alleviation of sepsis-induced myocardial injury and for enhancement of the protective effect of a liver X receptor agonist. PMID: 28993270
  42. reduction of Sirt1 activity restores adipogenesis in Sirt7(-/-) adipocytes in vitro and in vivo PMID: 28923965
  43. These results support previous work demonstrating that induction of SIRT1 in skeletal muscle, either at birth or in adulthood, does not impact muscle insulin action or mitochondrial function. PMID: 28544355
  44. we identified that Sirtuin 1 (SIRT1), a deacetylase that suppresses FoxO1 acetylation in granulosa cell (GCs), was downregulated by miR-181a and reversed the promoting effects of H2O2 and miR-181a on FoxO1 acetylation and GC apoptosis. PMID: 28981116
  45. the interaction of TIM4TIM1 was found to promote Th2cell proliferation through enhancing SIRT1 expression in mice with nasal allergic rhinitis. PMID: 28949386
  46. retinal vasculature from diabetic Sirt1 mice did not present any increase in the number of apoptotic cells or degenerative capillaries or decrease in vascular density. Diabetic Sirt1 mice were also protected from mitochondrial damage and had normal electroretinography responses and ganglion cell layer thickness. PMID: 29311218
  47. CKII-SIRT1-SM22alpha acts in a loop to reinforce the expression of SM22alpha, which limits the inflammatory response in vascular smooth muscle cells. PMID: 28419207
  48. SIRT1 may increase neuron function and resilience against Alzheimer's disease PMID: 27614878
  49. Regulation of stem cell aging by SIRT1 occurs via linking metabolic signaling to epigenetic modifications. (Review) PMID: 28392411
  50. Data show that 66-kDa Src homology 2 domain-containing protein (p66Shc) is acetylated under high glucose conditions and is deacetylated by Sirtuin1 lysine deacetylase (Sirt1) on lysine 81. PMID: 28137876

Show More

Hide All

Subcellular Location Nucleus, PML body, Cytoplasm, Nucleus
Protein Families Sirtuin family, Class I subfamily
Tissue Specificity Widely expressed. Weakly expressed in liver and skeletal muscle.
Database Links

KEGG: mmu:93759

STRING: 10090.ENSMUSP00000020257

UniGene: Mm.351459

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1