Rat Neurotrophin 3,NT-3 ELISA KIT

Instructions
Code CSB-E04688r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name neurotrophin 3
Alternative Names Ntf3 ELISA Kit; Ntf-3 ELISA Kit; Neurotrophin-3 ELISA Kit; NT-3 ELISA Kit; HDNF ELISA Kit; Nerve growth factor 2 ELISA Kit; NGF-2 ELISA Kit; Neurotrophic factor ELISA Kit
Abbreviation NTF3
Uniprot No. P18280
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.9 ng/mL-60 ng/mL
Sensitivity 0.225 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %88-96
1:2Average %94
Range %89-98
1:4Average %97
Range %93-101
1:8Average %95
Range %89-98
Recovery
The recovery of rat NT-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8882-94
EDTA plasma (n=4)9286-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
602.512 2.619 2.566 2.434
302.235 2.121 2.178 2.046
151.526 1.504 1.515 1.383
7.50.812 0.823 0.818 0.686
3.750.458 0.446 0.452 0.320
1.80.326 0.314 0.320 0.188
0.90.221 0.227 0.224 0.092
00.133 0.131 0.132
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Seems to promote the survival of visceral and proprioceptive sensory neurons.
Gene References into Functions
  1. the combined delivery of NT-3 and anti-NogoA from the DDS in an impact/compression model of SCI increases axon density and improves locomotor function. PMID: 27056081
  2. Compared to the sham group, the expression levels of NT-3 were significantly decreased and electroacupuncture was shown to upregulate the expression of NT-3 PMID: 27597902
  3. results demonstrate that BDNF and NT3 regulate axon initial segmen location and neuronal excitability PMID: 28440877
  4. Neurotrophin 3 activity in spinal cord was higher in balance and coordination trained animals following sciatic nerve crush compared to endurance trained. PMID: 26316168
  5. The expressed BDNF and NT3 exert a synergistic effect, which may promote the neuronal differentiation of adiposederived stem cells. PMID: 26239042
  6. These results indicate the feasibility of fabricating NT-3-immobilized scaffolds using the adsorption of NT-3/SF coating method, as well as the potential of these scaffolds to induce SCI repair by promoting survival and neuronal differentiation PMID: 25215612
  7. NT-3 gene-modified bone marrow-derived mesenchymal stem cells via lumbar puncture can strengthen the therapeutic benefits of bone mesenchymal stem cell transplantation PMID: 24744011
  8. A method of hypoglossal-facial nerve anastomosis through an NT3-expressing predegenerated nerve graft engineered by lentiviral vectors is designed to treat complete facial palsy in adult rats. PMID: 23581595
  9. Our results show that NT-3 modifies the ability of the mossy fiber pathway to present subsequent long-term potentiation PMID: 23387385
  10. mRNA expression of brain-derived neurotrophic factor (BDNF) and NT-3 (but not nerve growth factor) in injury segment follows neural stem cell transplantation and is remarkably upregulated. PMID: 22573254
  11. The exposure to long-term stress likely affects a variety of organs capable of releasing NT-3 into the bloodstream. Elevation of plasma NT-3 levels may play important roles in homeostasis under stress conditions. PMID: 23074106
  12. We conclude that upregulation of NT-3 in neonatally wounded skin is a critical factor mediating the sensory nerve sprouting that underlies hypersensitivity and pain following skin injury. PMID: 22871470
  13. Upregulation of neurotrophin-3 in PC12 cells reduces apoptosis under hypoxic conditions. PMID: 22960790
  14. The NTF3 gene promoted differentiation of neural stem cells into cholinergic neurons and enhanced neuronal cell survival PMID: 22971496
  15. Accompanying the simulated weightlessness extension, NT-3 expression in rat soleus muscle spindles is progressively decreased. PMID: 21340438
  16. Transient receptor potential canonical (TRPC)5 acts as a novel and specific mediator for NT-3 to regulate dendrite development through calcium/calmodulin-dependent protein kinase (CaMK)IIalpha. PMID: 22764246
  17. Muscle NT-3 levels increased by exercise training contribute to the improvement in caudal nerve conduction velocity in diabetic rats. PMID: 22552353
  18. Endogenous NT-3 may be involved in the analgesic effect of electroacupuncture on inflammatory pain in rats, mediated through the inhibition of spinal glial activity. PMID: 21680256
  19. Neurotrophin 3 modulates neuropathic pain in the dorsal root ganglia in a rat model. PMID: 21221027
  20. MiR21 is a candidate for regulating neurotrophin-3 signaling in the hippocampus following status epilepticus. PMID: 22019057
  21. neurotrophin 3 signaling pathway is essential for opiate-induced adaptation of locus coeruleus neurons. PMID: 17688944
  22. NT-3 immunoreactivity increased in activated fibers after partial transection of facial nerve. PMID: 21473141
  23. Neurotrophin 3 may lead to enhanced exocytosis of synaptic vesicles. PMID: 21370927
  24. regular rhythms of BDNF and NT-3 are essential for correct cortical network formation in juvenile rodents PMID: 21527636
  25. regrowth and perception were improved when the nerve graft overexpressed neurotrophin-3 at the time of transplantation PMID: 21135740
  26. Endogenous NT-3 has a distinctive role in promoting spiral ganglion axon growth in the organ of Corti and synaptogenesis on inner hair cells. PMID: 21613508
  27. Results suggest that proneurotrophin-3 and proBDNF may play important roles in the response to noise-induced injuries or ototoxic damage via the Sortilin:p75(NTR) death-signalling complex. PMID: 21261755
  28. 3'UTR of calreticulin has two conserved regions, and either of these is sufficient for axonal and dendritic targeting; only nucleotides 1315-1412 show ligand responsiveness to neurotrophin 3 (NT3) and myelin-associated glycoprotein (MAG) PMID: 20308067
  29. Report the proangiogenic capacity of NT-3 and propose NT-3 as a novel potential agent for the treatment of ischemic disease. PMID: 20360537
  30. Our results indicate for the first time that monoaminergic neurotransmitters play an important role in the regulation of NT-3 synthesis in cultured rat astrocytes. PMID: 19854260
  31. Brain-derived neurotrophic factor (BDNF) levels in the spinal cord, as detected by enzyme-linked immunosorbent assay, decreased with time, whereas neurotrophin-3 (NT-3) levels remained unchanged. PMID: 19203225
  32. exercise decreases hippocampal neurotrophin-3 PMID: 12914971
  33. ability of neurotrophin-3 (NT-3) to prevent abnormalities in neurofilamens in sensory neurons in experimental diabetes PMID: 12941778
  34. neurotrophin-3 (NT-3), delivered chronically via fibroblasts implanted intrathecally into neonatal rats, can facilitate synaptic transmission in the spinal cord PMID: 14507970
  35. Transfection of NT-3 into oligidendrocyte precursors caused a 10-fold increase in myelin basic protein production observed in myelin membranes in cocultures with hippocampal neurons. PMID: 15139015
  36. the level of supraspinal and muscle afferent input plays an important role in modulating the levels of BDNF and NT-3 in the spinal cord. PMID: 15548637
  37. These data demonstrate that localized overexpression of BDNF or NT-3, but not NGF, is sufficient to promote sprouting of serotonergic axons in the cortex following an experimental neurotoxic injury. PMID: 16206279
  38. BDNF, NT-3, and NGF, secreted by transplanted neural stem cells, were involved in the promotion of corticospinal axon growth after transplantation PMID: 17515814
  39. Glutamate receptor blockade inhibited the generation of spontaneous sodium-driven action potentials which, in turn, regulate both the endogenous secretion of NT-3 and the calbindin-D(28k) phenotype acquisition. PMID: 17561837
  40. neurotrophin-3 is a unique hypertrophic factor, which is paradoxically downregulated in cardiac hypertrophy and might counteract hypertrophic change PMID: 17628607
  41. Biochemically, the XYS decoction also reversed CIS-induced decreases in BDNF and increases in TrkB and NT-3 in the frontal cortex and the hippocampal CA(1) subregion. PMID: 17647101
  42. Vascular-derived NT-3 increases or maintains neuropeptide Y and tyrosine hydroxylase expression in postganglionic sympathetic neurons PMID: 18032527
  43. The functional consequences of the effect of IL-1beta on NT-3 signaling were severe, as NT-3 protection of the trophic support-deprived cortical neurons was abrogated. PMID: 18036576
  44. Increased NT-3 mRNA expression in hippocampus was detected in the immobilization stress group; the magnitude of increase in NT-3 mRNA was decreased when animals were allowed to bite. PMID: 18191115
  45. Co-culture of Ntf3 over-expressing Schwann cells with SGNs from early postnatal rats significantly enhanced neuronal survival in comparison to both control Schwann cells and conventional recombinant neurotrophin proteins. PMID: 18304740
  46. Non-viral vector-mediated genetic engineering of OEG was safe and more effective in producing NT-3 and promoting axonal outgrowth followed by enhancing SCI recovery in rats. PMID: 18369383
  47. Exposure of neurons to increased levels of NT-3 reduces expression of sodium channels Na(v)1.8 and Na(v)1.9 in dorsal root ganglia neurons subject to chronic constriction injury. PMID: 18601922
  48. levels in cerebellar vermis were higher for animals in an enriched environment after prenatal alcohol exposure PMID: 18652597
  49. Analysis of neurotrophin expression in dentate gyrus dosen't reveal an expression change of Ntf3 in a genetically hypertensive strain of Wistar rat. PMID: 18957307
  50. findings suggest that orexins, but not melanin-concentrating hormone, may be an inducer of NT-3 in the cerebral cortex PMID: 19026718
  51. Data show that the 14 kDa mature NT-3 species in the superior cervical ganglion are significantly reduced following axotomy. PMID: 19100726
  52. endogenous NT-3 could play an important role in spinal plasticity in adult spinal cords subjected to transection, possibly through a regulation of neuronal activity in the local circuitry PMID: 19513915

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Subcellular Location Secreted
Protein Families NGF-beta family
Tissue Specificity Brain and peripheral tissues.
Database Links

KEGG: rno:81737

STRING: 10116.ENSRNOP00000026685

UniGene: Rn.9715

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