Rat Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) ELISA kit

Instructions
Code CSB-EL009869RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Alternative Names Gpx4Phospholipid hydroperoxide glutathione peroxidase ELISA kit; PHGPx ELISA kit; EC 1.11.1.12 ELISA kit; Glutathione peroxidase 4 ELISA kit; GPx-4 ELISA kit; GSHPx-4 ELISA kit
Abbreviation GPX4
Uniprot No. P36970
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 4.68 μU/mL-300 μU/mL
Sensitivity 1.17 μU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat GPX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:200Average %85
Range %80-90
1:400Average %102
Range %97-107
1:800Average %87
Range %83-91
1:1600Average %97
Range %91-103
Recovery
The recovery of rat GPX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8380-86
EDTA plasma (n=4)9287-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μU/mlOD1OD2AverageCorrected
3002.588 2.597 2.593 2.439
1502.292 2.212 2.252 2.098
751.614 1.635 1.625 1.471
37.51.021 1.065 1.043 0.889
18.750.622 0.638 0.630 0.476
9.370.384 0.374 0.379 0.225
4.680.242 0.252 0.247 0.093
00.152 0.156 0.154
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Protects cells against membrane lipid peroxidation and cell death
Gene References into Functions
  1. Gpx4-catalyzed reduction of lipid hydroperoxides in lipid membranes has been described. PMID: 28709976
  2. MS/MS analysis of oxidized rat GPx4 complies with the predicted structure, an 8-membered ring, in which selenium is bound as selenenylamide to the protein backbone. PMID: 26163004
  3. mitochondria-targeted antioxidant SkQ1 (50 nmol/kg during 5 days) significantly increased the mRNA levels of Nrf2 transcription factor and Nrf2-induced genes encoding antioxidant enzymes SOD1, SOD2, CAT, and GPx4 in rat peripheral blood leukocytes. PMID: 26071777
  4. Our findings suggest that a decrease in mitochondrial GPx4 monomer and a functional loss of activity may be a contributing factor to the vulnerability of dopaminergic neurons in Parkinson's disease PMID: 23816523
  5. Rat Gpx4 is a moonlighting protein that functions both as a peroxidase, as well as a structural protein. PMID: 10464096
  6. Upon selenium fluctuation, the modulation of UGA recoding efficiency depends on the nature of the SECIS, with Gpx1 being more sensitive than Gpx4. PMID: 22614831
  7. activity in testis and epididymal spermatozoa of rats of different ages (phospholipid hydroperoxide glutathione peroxidase PHGPx, Gpx4). PMID: 11903656
  8. an important component of the mechanisms regulating inflammation PMID: 12397078
  9. gene is differentially spliced during the meiotic prophase and haploid cell phases of spermatogenesis PMID: 12533403
  10. PHGPx signals were detected in chromatoid bodies, clear nucleoplasm, mitochondria-associated material, mitochondrial aggregates, granulated bodies, and vesicles in residual bodies in addition to mitochondria, nuclei, and acrosomes during spermatogenesis. PMID: 12724282
  11. the nuclear PHGPx variant has a role in regulating cell division PMID: 12819198
  12. overexpression provides protection against damage from simulated ischemia/reoxygenation injury, particularly in the mitochondria, and the combination of mPHGPx and HSP60/10 imparts an added protective effect PMID: 14583338
  13. presence of traces of the larger snGPx form suggests that exhaustive proteolytic processing of the precursor produces the 25.9 kDa enzyme PMID: 15670826
  14. PHGPx activity in activated PMNs appears to be related to the appearance of the intrinsic function of PMNs in the inflammatory site. PMID: 15757501
  15. TGR and GPx4 can serve as a novel disulfide bond formation system and contain a catalytic selenocysteine consistent with the role of selenium in male reproduction PMID: 15901730
  16. PHGPx interacts with SMCP; the cysteine motif of PHGPx is a new substrate of the selenoperoxidase PMID: 16159880
  17. Astrocytic upregulation of GPx4 in response to injury is part of a protective cascade counteracting further cell damage in brain injury. PMID: 17603929
  18. We therefore propose that under persistent oxidative stress the formation of hepoxilin A3 and the HXA3-induced upregulation of PHGPx constitute a compensatory defense response to protect the vitality and functionality of the cell. PMID: 17997296
  19. liver and kidney Gpx4 activity in old female rats PMID: 19398061
  20. Data show that the intake of fish oil in the selenium-deficient animal led to a markedly altered formation of sperm nuclei glutathione peroxidase (snGPx), also designated as the nuclear form of PHGPx (n-PHGPx). PMID: 19723078

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Subcellular Location Mitochondrion, Cytoplasm
Protein Families Glutathione peroxidase family
Tissue Specificity Present primarily in testis (PubMed:1556123). Expressed in flagella of epididymal sperm (PubMed:19423663). Isoform Cytoplasmic: Highly expressed in testis (PubMed:14575705). Present in spermatogonia, spermatocyte and spermatid (at protein level) (PubMed:1
Database Links

KEGG: rno:29328

UniGene: Rn.3647

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