Rat Polypeptide YY(PYY)ELISA Kit

Code CSB-E13432r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
peptide YY
Alternative Names
PyyPeptide YY ELISA kit; PYY ELISA kit; Peptide tyrosine tyrosine) [Cleaved into: Peptide YY(3-36 ELISA kit; PYY-II)] ELISA kit
Abbreviation
PYY
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-100  
1:2 Average % 88  
Range % 84-93  
1:4 Average % 90  
Range % 85-94  
1:8 Average % 99  
Range % 94-105  
Recovery
The recovery of rat PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-101  
EDTA plasma (n=4) 100 95-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 2.478 2.374 2.426 2.318  
250 1.754 1.852 1.803 1.695  
125 1.188 1.226 1.207 1.099  
62.5 0.725 0.689 0.707 0.599  
31.2 0.403 0.382 0.393 0.285  
15.6 0.272 0.257 0.265 0.157  
7.8 0.189 0.178 0.184 0.076  
0 0.110 0.105 0.108    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This rat polypeptide YY (PYY) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat PYY in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PYY bound in the initial step.

PYY is an appetite-inhibiting gut hormone secreted from the L-cells of the gastrointestinal tract. Low levels of PYY are associated with higher BMI and obesity. Circulating PYY increases satiety and subsequently reduces food intake through gut-brain communication, and suppresses gastrointestinal motility and pancreatic hormone secretion. Circulating PYY levels increase during aerobic exercise. Additionally, PYY plays an integral part in maintaining energy homeostasis. PYY also plays an important role in the absorption of water and electrolytes.

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Target Background

Function
(From Uniprot)
This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
Gene References into Functions
  1. Peptide YY mediates the satiety effects of diets enriched with whey protein fractions in male rats PMID: 29042449
  2. Additional analysis of the findings demonstrated significant interactions of ovarian cycle stage and fed/fasted status with age on GLP-1, but not PYY plasma concentrations PMID: 28237410
  3. These findings indicate that the mechanism underlying type 2 diabetes remission may be mediated by PYY. PMID: 27117413
  4. Serum PYY levels were nonsignificantly decreased in rats with human neuroblastoma and hepatoblastoma transplants. PMID: 25111274
  5. gut PYY(3-36) action at Y2 receptors peripheral to the blood brain barrier plays an essential role in mediating satiety PMID: 23482449
  6. RACK1 modulates the ability of preglomerular vascular smooth muscle cells to respond to the proliferative actions of PYY. PMID: 23303411
  7. Exercise in hypoxia suppressed hunger and increased PYY but did not influence ad libitum energy intake. PMID: 22114179
  8. PYY concentrations in plasma, descending colon and rectum tissues were increased (P < 0.001) throughout pregnancy and lactation. PYY peaked at day 5 of lactation in plasma, as well as descending colon and rectum tissues. PMID: 19778563
  9. PYY induced a contraction of smooth muscle strips from proximal colon, but not on smooth muscle from any other gut segments. PMID: 12383864
  10. findings indicate that insulin-like growth factor-I functions as a positive regulator of peptide YY gene expression and that the stimulatory effect may be mediated by Sp1 proteins PMID: 14592960
  11. PYY(3-36) might play a role in the neuroendocrine modulation of the reproductive axis PMID: 15564330
  12. observations indicate that Peptide YY (PYY) levels are influenced by age, thyroid hormones, and growth hormone and indicate that PYY could be involved in the changes of food intake associated with these conditions PMID: 15687395
  13. study indicates that peripheral Peptide YY (PYY) may transmit satiety signals to the brain in part via the vagal afferent pathway PMID: 15718279
  14. PYY-immunoreactive (ir) cell bodies were localized exclusively to the gigantocellular reticular nucleus (Gi) of the rostral medulla. The highest density of PYY fibers was present within the solitary tract nucleus. PMID: 18022952
  15. Demonstrate the development of a potent homeostatic response to increase food intake when PYY(3-36) reduces food intake and energy reserves in diet-induced obese rats. PMID: 18550871

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Subcellular Location
Secreted.
Protein Families
NPY family
Database Links
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