24T ELISA Kit Trial Size (Only USD$150/ kit)
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|Intra-assay Precision (Precision within an assay): CV%<8%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<10%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of rat SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||91||85-97|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
The Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit is a powerful research tool designed to detect and quantify Pulmonary surfactant-associated protein B in rat serum, plasma, and tissue homogenates. This kit is specifically designed for use in lung-related disease and signal transduction research and can detect levels of SP-B as low as 0.78 pg/mL with a detection range of 3.12 pg/mL to 200 pg/mL.
The assay principle is based on the sandwich technique, in which the capture antibody is pre-coated on a microplate and the detection antibody is labeled with biotin. Upon binding to the target analyte, the complex is captured by streptavidin-HRP conjugate and the substrate TMB is added to produce a colored reaction product that is quantified by measuring the absorbance at 450 nm.
With an assay time of 1-5 hours, a sample volume of 50-100 µl, and a detection wavelength of 450 nm, the SP-B ELISA kit provides accurate and reliable results for your research needs. With its high sensitivity and wide detection range, it can help you gain a better understanding of the role of SP-B in various physiological and pathological conditions.
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