Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit

Code CSB-E12630r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
surfactant protein B
Alternative Names
Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
Abbreviation
SFTPB
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 pg/mL-200 pg/mL
Sensitivity
0.78 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %90
Range %85-100
1:2000Average %94
Range %87-98
1:4000Average %90
Range %85-96
1:8000Average %94
Range %90-98
Recovery
The recovery of rat SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-101
EDTA plasma (n=4)9185-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.470 2.589 2.530 2.377
1002.115 2.124 2.120 1.967
501.713 1.726 1.720 1.567
251.315 1.356 1.336 1.183
12.50.985 0.971 0.978 0.825
6.250.532 0.541 0.537 0.384
3.120.378 0.389 0.384 0.231
00.152 0.154 0.153  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit is a powerful research tool designed to detect and quantify Pulmonary surfactant-associated protein B in rat serum, plasma, and tissue homogenates. This kit is specifically designed for use in lung-related disease and signal transduction research and can detect levels of SP-B as low as 0.78 pg/mL with a detection range of 3.12 pg/mL to 200 pg/mL.

The assay principle is based on the sandwich technique, in which the capture antibody is pre-coated on a microplate and the detection antibody is labeled with biotin. Upon binding to the target analyte, the complex is captured by streptavidin-HRP conjugate and the substrate TMB is added to produce a colored reaction product that is quantified by measuring the absorbance at 450 nm.

With an assay time of 1-5 hours, a sample volume of 50-100 µl, and a detection wavelength of 450 nm, the SP-B ELISA kit provides accurate and reliable results for your research needs. With its high sensitivity and wide detection range, it can help you gain a better understanding of the role of SP-B in various physiological and pathological conditions.

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Target Background

Function
(From Uniprot)
Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
Gene References into Functions
  1. SP-B is present on the surface of exocytosed surfactant particles. Antibodies against SP-B stabilize the released particles when contacting the air-liquid interface, precluding their transformation and the formation of surface films. SP-B acts as an essential, preformed molecular key in the initial stages of surfactant unpacking and surface film formation. PMID: 27155084
  2. Liraglutide activates the GLP1 receptor, increasing ACE2 expression, reversing right ventricle hypertrophy, and improving the production of SP-A and SP-B in the lungs of Type 1 Diabetes rats PMID: 26196539
  3. The role of the SP-B NH(2) terminus in the formation and stability of cylindrical structures, specifically the distribution of lipid stack height, width, and density with four SP-B truncation peptides, was studied. PMID: 20023175
  4. VEGF increased expression of SP-b mRNA in preterm rat lung. PMID: 17267143
  5. alterations of surfactant are a consequence of the ventilation strategy that impair the biophysical activity of this material and thereby contribute directly to lung dysfunction over time PMID: 18344412

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Subcellular Location
Secreted, extracellular space, surface film.
Database Links
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Address
7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
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