Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit

Instructions
Code CSB-E12630r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name surfactant protein B
Alternative Names Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
Abbreviation SFTPB
Uniprot No. P22355
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %90
Range %85-100
1:2000Average %94
Range %87-98
1:4000Average %90
Range %85-96
1:8000Average %94
Range %90-98
Recovery
The recovery of rat SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-101
EDTA plasma (n=4)9185-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.470 2.589 2.530 2.377
1002.115 2.124 2.120 1.967
501.713 1.726 1.720 1.567
251.315 1.356 1.336 1.183
12.50.985 0.971 0.978 0.825
6.250.532 0.541 0.537 0.384
3.120.378 0.389 0.384 0.231
00.152 0.154 0.153  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
Gene References into Functions
  1. SP-B is present on the surface of exocytosed surfactant particles. Antibodies against SP-B stabilize the released particles when contacting the air-liquid interface, precluding their transformation and the formation of surface films. SP-B acts as an essential, preformed molecular key in the initial stages of surfactant unpacking and surface film formation. PMID: 27155084
  2. Liraglutide activates the GLP1 receptor, increasing ACE2 expression, reversing right ventricle hypertrophy, and improving the production of SP-A and SP-B in the lungs of Type 1 Diabetes rats PMID: 26196539
  3. The role of the SP-B NH(2) terminus in the formation and stability of cylindrical structures, specifically the distribution of lipid stack height, width, and density with four SP-B truncation peptides, was studied. PMID: 20023175
  4. VEGF increased expression of SP-b mRNA in preterm rat lung. PMID: 17267143
  5. alterations of surfactant are a consequence of the ventilation strategy that impair the biophysical activity of this material and thereby contribute directly to lung dysfunction over time PMID: 18344412

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Subcellular Location Secreted, extracellular space, surface film
Database Links

KEGG: rno:192155

STRING: 10116.ENSRNOP00000014505

UniGene: Rn.1952

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