Rat Sodium/potassium-transporting ATPase subunit alpha-1(ATP1A1) ELISA kit

Instructions
Code CSB-EL002322RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Alternative Names Atp1a1Sodium/potassium-transporting ATPase subunit alpha-1 ELISA kit; Na(+)/K(+) ATPase alpha-1 subunit ELISA kit; EC 7.2.2.13 ELISA kit; Sodium pump subunit alpha-1 ELISA kit
Abbreviation ATP1A1
Uniprot No. P06685
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.2 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat ATP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 83-90
1:2 Average % 102
Range % 98-106
1:4 Average % 86
Range % 83-89
1:8 Average % 98
Range % 94-103
Recovery
The recovery of rat ATP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 85 81-89
EDTA plasma (n=4) 98 94-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.623 2.601 2.612 2.464
1000 1.686 1.722 1.704 1.556
500 1.135 1.202 1.169 1.021
250 0.759 0.762 0.761 0.613
125 0.507 0.513 0.510 0.362
62.5 0.360 0.352 0.356 0.208
31.2 0.273 0.268 0.271 0.123
0 0.149 0.147 0.148  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Gene References into Functions
  1. An antibody against the extracellular DR region (897-911) of Na(+)-K(+)-ATPase subunit alpha 1 disrupted the Na+-K+-ATPase/ROS amplifier and protected cultured cardiomyocytes from ROS-induced injury. PMID: 28181111
  2. overexpression of ATP1A1 is strongly associated with the presence and severity of N-nitrosomethylbenzylamine- and arecoline-induced esophageal squamous cell carcinoma in rats PMID: 27845894
  3. Direct carbonylation modification of Pro224 in the rat alpha1 subunit determines ouabain-mediated Na/K-ATPase signal transduction and subsequent regulation of renal proximal tubule sodium transport. PMID: 27613772
  4. Herein potential interaction between NTS1 receptor, dopaminergic D2 receptor and Na(+), K(+)-ATPase was studied.Results indicated that typical and atypical antipsychotic drugs differentially modulate the interaction between neurotensin and Na(+), K(+)-ATPase. PMID: 27586561
  5. These results suggest that decreased renal NHERF-1 expression contributes to the impaired dopamine-mediated inhibition of NKA in PTCs from animal models of hypertension. PMID: 28515088
  6. In contrast to Na/K-ATPase alpha1, alpha3 could not regulate Src. Upon exposure to ouabain, Src activation did not occur, yet ERK was activated through Src-independent pathways involving PI3K and PKC. Hence, alpha3 expression confers signaling and pumping properties that are clearly distinct from that of cells expressing Na/K-ATPase alpha1. PMID: 27903584
  7. Data show that Na(+)/K(+)-ATPase's DR-region specific monoclonal antibody and direct activator DRm217 increased Na(+)/K(+)-ATPase activity and alleviated Ca(2+) level. PMID: 27563007
  8. signaling through Na/K-ATPase regulates miRNAs and specifically, miR-29b-3p expression both in vivo and in vitro. PMID: 26702050
  9. Phosphorylation at Ser(938) is part of the mechanism by which ANG II directly stimulates activity and trafficking of the rat kidney Na-K pump in opossum kidney cells. PMID: 26582472
  10. protein kinase A and C activation can increase Na,K-ATPase activity in human muscle but not via P2Y receptor stimulation. PMID: 24614174
  11. Data suggest that, in polycystic ovary syndrome, heart exhibits down-regulation of both Na+/K+-ATPase (Na+/K+ transporting ATPase alpha 1) and eNOS (nitric oxide synthase 3) activities, but up-regulation of iNOS (nitric oxide synthase 2) activity. PMID: 25988879
  12. the PI 3-kinase/PKB signaling pathway is involved in the IGF-I-sustained basal (Na(+)/K(+))-ATPase activity during the first 7 days of the postnatal development of rat retina. PMID: 25274047
  13. Data indicate that ethylmalonic acid (EMA) injection reduced Na(+),K(+)-ATPase activity and glutathione concentrations, whereas malondialdehyde levels were increased. PMID: 25583115
  14. Data indicate that long-term regulation involves transcription and translation of the Na/K pump alpha1/2-isoforms, and short-term regulation involves the translocation of the alpha1/2-isoforms to the plasma membrane. PMID: 24903141
  15. Chronic and direct silencing of basolateral Na-K-ATPase uniquely regulates brush border membrane Na absorptive pathways in intestinal epithelial cells. Specifically, SGLT1 is stimulated secondary to enhanced affinity of the cotransporter. PMID: 25652450
  16. Data show that chronic malnutrition increases affinity for Na+ in renal Na+-ATPase. PMID: 25283821
  17. Data indicate that the oral administration of colestipol reduced the cholesterol content and concomitantly inhibited the (Na(+)/K(+))ATPase. PMID: 23829947
  18. effect of ovariectomy on renal and systemic hemodynamic, electrolyte excretion and total and dephosphorylated Na(+),K(+)-ATPase alpha1 subunit in normotensive Wistar rats under a normal sodium or high sodium intake versus intact females PMID: 23327671
  19. Data indicate that overexpression of ouabain-insensitive rat Na(+)/K(+)-ATPase alpha1 failed to inhibit internalization of human Na(+)/K(+)-ATPase alpha1 expressed in the same cells. PMID: 24275648
  20. Suggest that the protective effect produced by increased expression of NKA-alpha2 on the heart after pressure overload is due to more efficient Ca2+ clearance because this isoform of NKA preferentially enhances NCX1 activity compared with NKA-alpha1. PMID: 24218169
  21. Data indicate that exposure to cobalt (Co) decreased Na(+)K(+)-ATPase activity in the cerebrum and cerebellum of suckling pups. PMID: 23857379
  22. genetic knockdown of the Na(+),K(+)-ATPase alpha1 subunit blocks peptide and starvation-induced autosis in vitro PMID: 24277826
  23. Mild hyperhomocysteinemia significantly decreases the activity and the content of the alpha 1 and alpha 2 subunits of the Na(+),K(+)-ATPase in cerebral cortex and hippocampus of adult rats. PMID: 23467881
  24. Identification of an ATP1A1 mutant that has normal pumping function but is defective in signal transduction. PMID: 23532853
  25. Expression of mutant alpha1 Na/K-ATPase defective in conformational transition attenuates Src-mediated signal transduction PMID: 23288841
  26. there is a specific and essential role for Na,K-ATPase alpha3 in neurons co-expressing alpha1 and alpha3 PMID: 23195960
  27. The present study demonstrated that sepsis induced by cecal ligation and puncture inhibits Na(+), K(+)-ATPase activity in a mechanism dependent on oxidative stress. PMID: 22810802
  28. Maintenance of Na(+),K(+)-ATPase cell surface abundance is critical to myocyte survival after an ischemic attack and plays a role in ouabain-induced myocardial protection. PMID: 23086991
  29. regulatory S-glutathionylation of the catalytic subunit plays a key role in the redox-induced regulation of Na,K-ATPase activity PMID: 22798075
  30. estrogen and progesterone act in renal tissues modulating CNG-A1 and Na/K ATPase gene expression PMID: 22759964
  31. in the kidney cortex of rats with renovascular hypertension there is increased expression of Na,K-ATPase and a selective increase in its phosphorylation at Ser-11 that could increase the capacity to reabsorb sodium and water. PMID: 22237155
  32. Angiotensin II changes the conformation of two forms of the sodium potassium ATPase alpha 1 subunit via differential phosphorylation PMID: 22145807
  33. PP2A inhibits association between the Na+,K+-ATPase and arrestin, and diminishes the effect of arrestin on Na+,K+-ATPase trafficking. PMID: 22242112
  34. Data suggest that during hypoxia, calcium entry via CRAC channels leads to AMPK activation, Na,K-ATPase downregulation, and alveolar epithelial dysfunction. PMID: 21730292
  35. These data strongly suggest the oxidative damage as one possible mechanism involved in the reduction of Na(+),K(+)-ATPase activity caused by hypermethioninemia. PMID: 21354298
  36. The abundance of Na-K-ATPase proteins was significantly decreased in basolateral membranes of type II alveolar epithelial cells in sepsis. PMID: 21478253
  37. Altered Na(+),K(+)-ATPase renal function may precede the development of age-related pathologies and loss of renal function. PMID: 20883770
  38. The unchanged expression of Na(+),K(+)-ATPase alpha1-subunit in both genders indicates that 8 weeks represent the time when the mobilization of enzyme synthesis observed previously in acute diabetes is lost. PMID: 20817950
  39. trafficking of the newly synthesized Na,K-ATPase is regulated by association with beta-COP PMID: 20801885
  40. these results suggest that the NH(2)-terminus of KCC3a is a key region for association with alpha1NaK, and that KCC3a but not KCC3b can regulate the Na(+),K(+)-ATPase activity. PMID: 20691666
  41. Results suggest that the association of NHE-1 with Na-K-ATPase is critical for ouabain-mediated regulation of Na-K-ATPase and that these effects may play a role in cardioglycoside-stimulated hypertension. PMID: 20427472
  42. Myosin-Va has a role in restraining Na(+)/K(+)-ATPase-containing vesicles within intracellular pools. PMID: 19808891
  43. In conclusion, pump activity not only affects neural activity directly but our results also suggest that pump activity is affected through functional interaction with DOR that will modulate pain sensation. PMID: 19619588
  44. Atp1a1 activity in VSMC and REC cells of rats, dog, and human show that Atp1a1 does not mediate the involvement of ouabain in the development of hypertension in rats, suggesting that the pathogenesis of human and rat hypertension may differ. PMID: 11926353
  45. FXYD7 decreases the apparent K(+) affinity of rat alpha 1-beta 1 and alpha 2-beta 1, but not of alpha 3-beta1 isozymes. PMID: 12093728
  46. alpha(1)- and alpha(2)-isoforms expressed under the same conditions suggest an involvement of the central ISR in the response to PKC but not in K(+) deocclusion. PMID: 12372782
  47. Interactions between Na,K-ATPase alpha-subunit ATP-binding domains may play a role in cell function and in modulating interactions between the Na,K-ATPase and other proteins PMID: 12511576
  48. Ang II modulates Na(+)-K(+)ATPase activity in PC-Cl3 cells through the AT1 receptor via activation of atypical PKC-zeta while the Ang II-activated PKC appears to have other as yet unknown functions. PMID: 12527732
  49. Chronic heart failure-induced alterations in skeletal muscle Na(+)-K(+)-ATPase, including B(max) and isoform expression, can be partially reversed by exercise training. PMID: 12562669
  50. expression of Na/K ATPase alpha1 subunit mRNA was transiently increased in astrocytes after reoxygenation, whereas hypoxia itself did not induce any gene expression change. PMID: 12573531

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Subcellular Location Cell membrane, sarcolemma, Multi-pass membrane protein
Protein Families Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily
Database Links

KEGG: rno:24211

STRING: 10116.ENSRNOP00000045650

UniGene: Rn.217534

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