Rat Sodium/potassium-transporting ATPase subunit alpha-1(ATP1A1) ELISA kit

Instructions
Code CSB-EL002322RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Alternative Names Atp1a1Sodium/potassium-transporting ATPase subunit alpha-1 ELISA kit; Na(+)/K(+) ATPase alpha-1 subunit ELISA kit; EC 7.2.2.13 ELISA kit; Sodium pump subunit alpha-1 ELISA kit
Abbreviation ATP1A1
Uniprot No. P06685
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.2 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat ATP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 83-90
1:2 Average % 102
Range % 98-106
1:4 Average % 86
Range % 83-89
1:8 Average % 98
Range % 94-103
Recovery
The recovery of rat ATP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 85 81-89
EDTA plasma (n=4) 98 94-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.623 2.601 2.612 2.464
1000 1.686 1.722 1.704 1.556
500 1.135 1.202 1.169 1.021
250 0.759 0.762 0.761 0.613
125 0.507 0.513 0.510 0.362
62.5 0.360 0.352 0.356 0.208
31.2 0.273 0.268 0.271 0.123
0 0.149 0.147 0.148  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Gene References into Functions
  1. An antibody against the extracellular DR region (897-911) of Na(+)-K(+)-ATPase subunit alpha 1 disrupted the Na+-K+-ATPase/ROS amplifier and protected cultured cardiomyocytes from ROS-induced injury. PMID: 28181111
  2. overexpression of ATP1A1 is strongly associated with the presence and severity of N-nitrosomethylbenzylamine- and arecoline-induced esophageal squamous cell carcinoma in rats PMID: 27845894
  3. Direct carbonylation modification of Pro224 in the rat alpha1 subunit determines ouabain-mediated Na/K-ATPase signal transduction and subsequent regulation of renal proximal tubule sodium transport. PMID: 27613772
  4. Herein potential interaction between NTS1 receptor, dopaminergic D2 receptor and Na(+), K(+)-ATPase was studied.Results indicated that typical and atypical antipsychotic drugs differentially modulate the interaction between neurotensin and Na(+), K(+)-ATPase. PMID: 27586561
  5. These results suggest that decreased renal NHERF-1 expression contributes to the impaired dopamine-mediated inhibition of NKA in PTCs from animal models of hypertension. PMID: 28515088
  6. In contrast to Na/K-ATPase alpha1, alpha3 could not regulate Src. Upon exposure to ouabain, Src activation did not occur, yet ERK was activated through Src-independent pathways involving PI3K and PKC. Hence, alpha3 expression confers signaling and pumping properties that are clearly distinct from that of cells expressing Na/K-ATPase alpha1. PMID: 27903584
  7. Data show that Na(+)/K(+)-ATPase's DR-region specific monoclonal antibody and direct activator DRm217 increased Na(+)/K(+)-ATPase activity and alleviated Ca(2+) level. PMID: 27563007
  8. signaling through Na/K-ATPase regulates miRNAs and specifically, miR-29b-3p expression both in vivo and in vitro. PMID: 26702050
  9. Phosphorylation at Ser(938) is part of the mechanism by which ANG II directly stimulates activity and trafficking of the rat kidney Na-K pump in opossum kidney cells. PMID: 26582472
  10. protein kinase A and C activation can increase Na,K-ATPase activity in human muscle but not via P2Y receptor stimulation. PMID: 24614174
  11. Data suggest that, in polycystic ovary syndrome, heart exhibits down-regulation of both Na+/K+-ATPase (Na+/K+ transporting ATPase alpha 1) and eNOS (nitric oxide synthase 3) activities, but up-regulation of iNOS (nitric oxide synthase 2) activity. PMID: 25988879
  12. the PI 3-kinase/PKB signaling pathway is involved in the IGF-I-sustained basal (Na(+)/K(+))-ATPase activity during the first 7 days of the postnatal development of rat retina. PMID: 25274047
  13. Data indicate that ethylmalonic acid (EMA) injection reduced Na(+),K(+)-ATPase activity and glutathione concentrations, whereas malondialdehyde levels were increased. PMID: 25583115
  14. Data indicate that long-term regulation involves transcription and translation of the Na/K pump alpha1/2-isoforms, and short-term regulation involves the translocation of the alpha1/2-isoforms to the plasma membrane. PMID: 24903141
  15. Chronic and direct silencing of basolateral Na-K-ATPase uniquely regulates brush border membrane Na absorptive pathways in intestinal epithelial cells. Specifically, SGLT1 is stimulated secondary to enhanced affinity of the cotransporter. PMID: 25652450
  16. Data show that chronic malnutrition increases affinity for Na+ in renal Na+-ATPase. PMID: 25283821
  17. Data indicate that the oral administration of colestipol reduced the cholesterol content and concomitantly inhibited the (Na(+)/K(+))ATPase. PMID: 23829947
  18. effect of ovariectomy on renal and systemic hemodynamic, electrolyte excretion and total and dephosphorylated Na(+),K(+)-ATPase alpha1 subunit in normotensive Wistar rats under a normal sodium or high sodium intake versus intact females PMID: 23327671
  19. Data indicate that overexpression of ouabain-insensitive rat Na(+)/K(+)-ATPase alpha1 failed to inhibit internalization of human Na(+)/K(+)-ATPase alpha1 expressed in the same cells. PMID: 24275648
  20. Suggest that the protective effect produced by increased expression of NKA-alpha2 on the heart after pressure overload is due to more efficient Ca2+ clearance because this isoform of NKA preferentially enhances NCX1 activity compared with NKA-alpha1. PMID: 24218169
  21. Data indicate that exposure to cobalt (Co) decreased Na(+)K(+)-ATPase activity in the cerebrum and cerebellum of suckling pups. PMID: 23857379
  22. genetic knockdown of the Na(+),K(+)-ATPase alpha1 subunit blocks peptide and starvation-induced autosis in vitro PMID: 24277826
  23. Mild hyperhomocysteinemia significantly decreases the activity and the content of the alpha 1 and alpha 2 subunits of the Na(+),K(+)-ATPase in cerebral cortex and hippocampus of adult rats. PMID: 23467881
  24. Identification of an ATP1A1 mutant that has normal pumping function but is defective in signal transduction. PMID: 23532853
  25. Expression of mutant alpha1 Na/K-ATPase defective in conformational transition attenuates Src-mediated signal transduction PMID: 23288841
  26. there is a specific and essential role for Na,K-ATPase alpha3 in neurons co-expressing alpha1 and alpha3 PMID: 23195960
  27. The present study demonstrated that sepsis induced by cecal ligation and puncture inhibits Na(+), K(+)-ATPase activity in a mechanism dependent on oxidative stress. PMID: 22810802
  28. Maintenance of Na(+),K(+)-ATPase cell surface abundance is critical to myocyte survival after an ischemic attack and plays a role in ouabain-induced myocardial protection. PMID: 23086991
  29. regulatory S-glutathionylation of the catalytic subunit plays a key role in the redox-induced regulation of Na,K-ATPase activity PMID: 22798075
  30. estrogen and progesterone act in renal tissues modulating CNG-A1 and Na/K ATPase gene expression PMID: 22759964
  31. in the kidney cortex of rats with renovascular hypertension there is increased expression of Na,K-ATPase and a selective increase in its phosphorylation at Ser-11 that could increase the capacity to reabsorb sodium and water. PMID: 22237155
  32. Angiotensin II changes the conformation of two forms of the sodium potassium ATPase alpha 1 subunit via differential phosphorylation PMID: 22145807
  33. PP2A inhibits association between the Na+,K+-ATPase and arrestin, and diminishes the effect of arrestin on Na+,K+-ATPase trafficking. PMID: 22242112
  34. Data suggest that during hypoxia, calcium entry via CRAC channels leads to AMPK activation, Na,K-ATPase downregulation, and alveolar epithelial dysfunction. PMID: 21730292
  35. These data strongly suggest the oxidative damage as one possible mechanism involved in the reduction of Na(+),K(+)-ATPase activity caused by hypermethioninemia. PMID: 21354298
  36. The abundance of Na-K-ATPase proteins was significantly decreased in basolateral membranes of type II alveolar epithelial cells in sepsis. PMID: 21478253
  37. Altered Na(+),K(+)-ATPase renal function may precede the development of age-related pathologies and loss of renal function. PMID: 20883770
  38. The unchanged expression of Na(+),K(+)-ATPase alpha1-subunit in both genders indicates that 8 weeks represent the time when the mobilization of enzyme synthesis observed previously in acute diabetes is lost. PMID: 20817950
  39. trafficking of the newly synthesized Na,K-ATPase is regulated by association with beta-COP PMID: 20801885
  40. these results suggest that the NH(2)-terminus of KCC3a is a key region for association with alpha1NaK, and that KCC3a but not KCC3b can regulate the Na(+),K(+)-ATPase activity. PMID: 20691666
  41. Results suggest that the association of NHE-1 with Na-K-ATPase is critical for ouabain-mediated regulation of Na-K-ATPase and that these effects may play a role in cardioglycoside-stimulated hypertension. PMID: 20427472
  42. Myosin-Va has a role in restraining Na(+)/K(+)-ATPase-containing vesicles within intracellular pools. PMID: 19808891
  43. In conclusion, pump activity not only affects neural activity directly but our results also suggest that pump activity is affected through functional interaction with DOR that will modulate pain sensation. PMID: 19619588
  44. Atp1a1 activity in VSMC and REC cells of rats, dog, and human show that Atp1a1 does not mediate the involvement of ouabain in the development of hypertension in rats, suggesting that the pathogenesis of human and rat hypertension may differ. PMID: 11926353
  45. FXYD7 decreases the apparent K(+) affinity of rat alpha 1-beta 1 and alpha 2-beta 1, but not of alpha 3-beta1 isozymes. PMID: 12093728
  46. alpha(1)- and alpha(2)-isoforms expressed under the same conditions suggest an involvement of the central ISR in the response to PKC but not in K(+) deocclusion. PMID: 12372782
  47. Interactions between Na,K-ATPase alpha-subunit ATP-binding domains may play a role in cell function and in modulating interactions between the Na,K-ATPase and other proteins PMID: 12511576
  48. Ang II modulates Na(+)-K(+)ATPase activity in PC-Cl3 cells through the AT1 receptor via activation of atypical PKC-zeta while the Ang II-activated PKC appears to have other as yet unknown functions. PMID: 12527732
  49. Chronic heart failure-induced alterations in skeletal muscle Na(+)-K(+)-ATPase, including B(max) and isoform expression, can be partially reversed by exercise training. PMID: 12562669
  50. expression of Na/K ATPase alpha1 subunit mRNA was transiently increased in astrocytes after reoxygenation, whereas hypoxia itself did not induce any gene expression change. PMID: 12573531
  51. Ischemic acute renal failure leads to down regulation of pulmonary ENaC, Na,K-ATPase and aquaporin-5. PMID: 12631124
  52. recruitment of new Na(+),K(+)-ATPase molecules into the plasma membrane appears to be a major mechanism by which dopamine increases total cell Na(+),K(+)-ATPase activity PMID: 12631730
  53. evidence that in medullary thick ascending limb, C-peptide stimulates Na(+),K(+)-ATPase activity within a physiological concentration; effect due to increase in Na(+),K(+)-ATPase turnover rate likely mediated by protein kinase C-alpha phosphorylation PMID: 12637991
  54. we report two high-resolution NMR structures of the 213-residue nucleotide-binding domain of rat alpha1 Na,K-ATPase, determined in the absence and the presence of ATP. PMID: 12730684
  55. The association of hypertensive status with the Lngfr gene was found only in young ISIAH rats whereas in adult rats of this line, hypertension was associated with the Atp1a1 locus PMID: 12884521
  56. all 3 amino acids appeared to be required for ouabain action, however, docking of ouabain onto a three-dimensional-model of Na+,K+-ATPase suggests that Asp804, in contrast to Phe783 and Thr797, does not actually form part of the ouabain-binding pocket PMID: 12972417
  57. sequence 442GDASE446 in the ATP binding pocket of Na/K-ATPase is an important motif and is involved in both the high and low affinity ATP effects rather than in free Mg2+, Na+, and K+ effects PMID: 14522987
  58. Data suggest that activation of both Src and phosphoinositide 3-kinase (PI3K) is required for the T3-induced stimulation of Na,K-ATPase activity and its cell surface. expression in adult rat alveolar epithelial cells. PMID: 15342623
  59. E2/E2P conformational states of Na+,K+-ATPase are stabilized by interactions between the catalytic site and the A-M3 linker PMID: 15574410
  60. Thr-774 (transmembrane segment M5), Val-920 (M8), and Glu-954 (M9) are involved in Na+ transport, and Gln-923 (M8) is essential for Na,K-ATPase activity PMID: 15764602
  61. Data demonstrate the existence and functional significance of the Q276L variant in Dahl S rats PMID: 15775778
  62. presentation of a homology model for the ouabain-binding site of Na,K-ATPase involving all seven amino acids as well as several earlier postulated amino acids PMID: 16051601
  63. location of the third sodium-specific site of the sodium pump in a space between the fifth, sixth, and ninth transmembrane helices of the alpha-subunit PMID: 16123128
  64. Our data suggest that the difference in the activity of Na(+)/K(+)-ATPase in the hippocampus is innate and is involved in the expression of the rearing behavior. PMID: 16153614
  65. the the TM domain of FXYD7 has a role in mediating the complex interactions of FXYD7 with Na,K-ATPase PMID: 16269407
  66. Redox state is a potent regulator of the Na-K-ATPase function. Shifts from an "optimal redox potential range" to higher or lower levels cause suppression of the Na-K pump activity. PMID: 16293684
  67. Data describe the interactions of rat FXYD1,-2, and -4 proteins with rat alpha1 subunits of Na(+),K(+)-ATPase. PMID: 16373350
  68. identified a well conserved Na,K-ATPase catalytic alpha-subunit motif that binds to the inositol 1,4,5-trisphosphate receptor and can trigger an anti-apoptotic calcium signal. PMID: 16723354
  69. critical conformational change in N-terminus during G protein coupled receptor-dependent endocytosis PMID: 16949583
  70. arterial vascular endothelium only expresses NaKalpha1, whereas vascular smooth muscle expresses NaKalpha1 and NaKalpha2, but not NaKalpha3 PMID: 17008770
  71. NKA-alpha1 is almost uniformly distributed between the T-tubules and external sarcolemma in ventricular cardiomyocytes. PMID: 17392375
  72. Cyclic stretch of skeletal muscle cells induced translocation of Na(+)-K(+)-ATPase alpha(1)- and alpha(2)-subunits to the plasma membrane. PMID: 17458903
  73. Increasing smooth muscle Atp1a1/2 decreases blood pressure and is dependent on the degree of increased alpha-isoform expression. PMID: 17468335
  74. The present study was undertaken to investigate the ultrastructural localization and distribution of Na,K-ATPase in peripheral nerve fibers. PMID: 17507069
  75. Ubiquitination plays an important role in cellular adaptation to hypoxia by regulating Na,K-ATPase alpha(1)-subunit endocytosis. PMID: 17532187
  76. Spermidine induction of Atp1a1 in small intestine may be mediated by altered ornithine decarboxylase expression. PMID: 17673438
  77. H(2)O(2) may be a modulator of jejunal ion and water transport by multiple mechanisms, among which a significant inhibition of the basolateral (Na+, K+) -ATPase. PMID: 17880911
  78. 31KKE in H1 and 47HRK in H2 of the Na,K-ATPase have distinct roles in modulating the enzyme's conformational transitions during the catalytic cycle of the enzyme PMID: 17881356
  79. review mutagenesis work on the structure-function relationship of transmembrane segment M1 in the Na+,K+-ATPase and the sarco(endo)plasmic reticulum Ca2+-ATPase PMID: 18058007
  80. KCC3a forms a functional complex with alpha1NaK in the basolateral membrane of luminal parietal cells, and it up-regulates alpha1NaK in lipid rafts, whereas KCC3a is absent in basal parietal cells PMID: 18178552
  81. Although it was possible to readily detect other silent polymorphisms between salt sensensitive Dahl and salt resistant strains in the Atp1a1 gene by all methods used, no evidence for the existence of the A1079T transversion in SS/Jr rats was found PMID: 18285611
  82. Identification and function of a cytoplasmic K+ site of the Na+, K+ -ATPase. PMID: 18669634
  83. The fourth extracellular loop of the alpha subunit of Na,K-ATPase. Functional evidence for close proximity with the second extracellular loop. PMID: 18693246
  84. increased intracellular sodium concentration induces the increased colocalization of dopamine receptors with Na-K-ATPase molecules in the region of the plasma membrane PMID: 18701625
  85. Ankyrin facilitates intracellular trafficking of alpha1-Na+-K+-ATPase in polarized cells. PMID: 18768923
  86. Results suggest that Na/K-ATPase regulates Caveolin-1 endocytic trafficking and stabilizes the Cav1 plasma membrane pool. PMID: 18794328
  87. In organ of Corti and spiral ganglion, NKA alpha1 is expressed in the supporting cells that neighbor the inner hair cells and express the glutamate transporter GLAST. PMID: 19082858
  88. Na,K-ATPase signal transduction triggers CREB activation and dendritic growth PMID: 19164762
  89. Altered renal expression of Atp1a1 in protein-deprived rats. PMID: 19202345
  90. The plasma membrane Na/K-ATPase-caveolin-1 interaction may represent an important sensing mechanism by which the cells regulate the sterol regulatory element-binding protein pathway. PMID: 19363037
  91. Data suggest that hypoxia-generated mitochondrial reactive oxygen species lead to the activation of the AMPK alpha1 isoform, which binds and directly phosphorylates PKC zeta at Thr410, thereby promoting Na,K-ATPase endocytosis. PMID: 19380482
  92. NKA may act as a microtubule-plasma membrane anchorage site through an interaction between acetylated tubulin and cytoplasmic domain 5. PMID: 19476441
  93. The alpha1-alpha 3 subunits of Na,K-ATPase were detected, as were the glutamate transporters GLAST and GLT-1, demonstrating that glutamate transporters copurify with Na,K-ATPases. PMID: 19553454

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Subcellular Location Cell membrane, sarcolemma, Multi-pass membrane protein
Protein Families Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily
Database Links

KEGG: rno:24211

STRING: 10116.ENSRNOP00000045650

UniGene: Rn.217534

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