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Rat Testosterone,T ELISA Kit

Rat Testosterone,T ELISA Kit

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Code	CSB-E05100r
Size	96T,5×96T,10×96T
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Product Details

Target Name

Testosterone,T

Alternative Names

N/A

Abbreviation

T

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.13 ng/mL-25.6 ng/mL

Sensitivity

0.06 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat testosteronen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		86-96
1:2		Average %		97
		Range %		92-104
1:4		Average %		85
		Range %		81-88
1:8		Average %		89
		Range %		83-93

Recovery

The recovery of rat testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		90		87-95
EDTA plasma (n=4)		94		90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
25.6	0.238	0.236	0.237
6.4	0.435	0.431	0.433
2	0.611	0.615	0.613
0.5	0.984	0.982	0.983
0.13	1.403	1.398	1.401
0	2.001	2.003	2.002

Troubleshooting
and FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Citations

Effect of Iron Oxide Nanopartical on The FSH, LH and Testesteron Hormones in The offspring of Albino Rats RH Kamel,Indian Journal of Forensic Medicine & Toxicology,2020
Irisin may have a role in pubertal development and regulation of reproductive function in rats N Ulker,Reproduction,2020
The Role of Testosterone in Amplifying the Effect of a Phosphodiesterase Type 5 Inhibitor After Pelvic Irradiation DS Lee,The Journal of Sexual Medicine,2020
Influence of malignant growth and chronic neurogenic pain on neurosteroid levels in rat brain EM Frantsiyants,Biomeditsinskaya Khimiya,2020
Eurycoma longifolia Root Aqueous Extract Augments the Testosterone Level and Spermatogenesis in Adult Male Rats Al-Faqeha H H, et al,/,2019
THE IMPACT OF 2-AMINOETHANESULFONIC ACID, GUARANINE METHYL THEOBROMINE 1, 3, 7-TRIMETHYLXANTHINE THEINE AND/OR ISOBUTYLPHENYL PROPIONIC ACID ON MASCULINE FECUNDITY, AND MORPHOPATHOLOGICAL DISORDERS IN RATS DAYYIH W A B U, et al,Asian J Pharm Clin Res,2019
Recombinant B2L and Kisspeptin-54 DNA Vaccine Induces Immunity Against Orf Virus and Inhibits Spermatogenesis In Rats Wassie T, et al,Scientific Reports,2019
PROTECTIVE EFFECTS OF CACTUS AND/OR PAPAYA JUICES AGAINST HEPATIC AND TESTICULAR TOXICITY INDUCED BY CHLORPYRIFOS IN ALBINO RATS Mogahed H E, et al,World Journal of Pharmaceutical Research,2019
Protective efficiency of Ashrasi date palm hydroalcoholic extract against diabetes-induced testicular toxicity: A biochemical and stereological study Hosseinipour M, et al,andrologia,2019
Mechanisms of Stress-Induced Spermatogenesis Impairment in Male Rats Following Unpredictable Chronic Mild Stress (uCMS) Zou P, et al,International Journal of Molecular Sciences,2019
Effects of Osteocalcin on Synthesis of Testosterone and INSL3 during Adult Leydig Cell Differentiation Gulfidan Coskun, et al,International Journal of Endocrinology,2019
Crotonaldehyde-induced alterations in testicular enzyme function and hormone levels, and apoptosis in the testes of male Wistar rats are associated with oxidative damage Zhang B, et al,Toxicology Mechanisms and Methods,2019
Seasonal expressions of androgen receptor, estrogen receptors, 5α-reductases and P450arom in the epididymis of the male muskrat (Ondatra zibethicus) Liu Q, et al,Journal of Steroid Biochemistry and Molecular Biology,2019
The influence of TRAIL, adiponectin and sclerostin alterations on bone loss in BDL-induced cirrhotic rats and the effect of opioid system blockade Moradi M, et al,Life Sciences,2019
Ocimum kilimandscharicum L. restores ovarian functions in letrozole - induced Polycystic Ovary Syndrome (PCOS) in rats: Comparison with metformin Khaled N, et al,Life Sciences,2019
Fluvastatin attenuates doxorubicin-induced testicular toxicity in rats by reducing oxidative stress and regulating the blood-testis barrier via mTOR signaling pathway Gurel C, et al,Human & Experimental Toxicology,2019
ETA-mediated anti-TNF-α therapy ameliorates the phenotype of PCOS model induced by letrozole Lang Q,PLoS One.,2019
Prenatal Androgenization Induces Anxiety-Like Behavior in Female Rats, Associated with Reduction of Inhibitory Interneurons and Increased BDNF in Hippocampus and Cortex Bojana Rankov Petrovic,BIOMED RESEARCH INTERNATIONAL,2019
Finasteride-Induced Inhibition of 5α-Reductase Type 2 Could Lead to Kidney Damage-Animal, Experimental Study Baig MS,International Journal of Environmental Research and Public Health,2019
Phosphodiesterase 4 inhibitor plus metformin is superior to metformin alone for the treatment of polycystic ovary syndrome: A rat model study Yusuf Aytac Tohma, et al,Experimental and Therapeutic Medicine,2019
Impact of Hydroalcoholic Extract of Humulus Lupulus L. on Sperm Quality, Reproductive Organs and Hormones in Male Rat Karbalaei N, et al,Chinese journal of integrative medicine,2019
Prenatal exposure to testosterone induces cardiac hypertrophy in adult female rats through enhanced Pkcδ expression in cardiac myocytes Hou M, et al,Journal of Molecular and Cellular Cardiology,2019
Impact of Sleep Deprivation on the Hypothalamic-Pituitary-Gonadal Axis and Erectile Tissue Lee DS, et al,Journal of Sexual Medicine,2019
Venlafaxine and carvedilol ameliorate testicular impairment and disrupted spermatogenesis in rheumatoid arthritis by targeting AMPK/ERK and PI3K/AKT/mTOR pathways Eid AH, et al,Toxicology and applied pharmacology,2018
Venlafaxine and carvedilol ameliorate testicular impairment and disrupted spermatogenesis in rheumatoid arthritis by targeting AMPK/ERK and PI3K/AKT/mTOR pathways Eid AH, et al,Toxicology and applied pharmacology,2018
Amelioration of diabetes‐induced testicular and sperm damage in rats by cerium oxide nanoparticle treatment Tayebe Artimani.et al,ANDROLOGIA,2018
TOXIC EFFECTS OF TRAZADONE ON MALE REPRODUCTIVE SYSTEM VIA DISRUPTING HYPOTHALAMIC-PITUITARY-TESTICULAR AXIS AND INDUCING TESTICULAR OXIDATIVE STRESS Sinem Ilgin.et al,/,2018
Protective effects of persian honey, Apis Mellifera Meda Skorikov on side effects of chemotherapy and ischemia/reperfusion induced testicular injury Mohammadreza Gholami .et al,Journal of Complementary and Integrative Medicine,2018
Antioxidant activity of Spirulina platensis alleviates doxorubicin-induced oxidative stress and reprotoxicity in male rats Naglaa Z. H.et al,Oriental Pharmacy and Experimental Medicine,2018
Serum and Brain Tissue Metabolic Profile Changes on Rat Model of Sleep Deprivation and Intervention Effects of Total Ginsenoside Using Metabonomic Xiao-jun Gou1.et al,Center for Chinese Medicine Therapy and Systems Biology,/
Beneficial effect of folic acid on kidney and testes of adult albino rats after exposure to methomyl Samar Sakr.et al,Toxicol. Res,2018
Serum and Brain Metabolomic Variations Reveal Perturbation of Sleep Deprivation on Rats and Ameliorate Effect of Total Ginsenoside Treatment Xiao-jun Gou.et al,International Journal of Genomics,2017
Lactational exposure of polychlorinated biphenyls impairs Leydig cellular steroidogenesis in F1 progeny rats Thangavelu SK.et al,Reprod Toxicol,2017
Investigating the effects of testosterone on uterine fluid regulation and endometrial receptivity in a rat model / Mohd Helmy bin Mokhtar Mohd Helmy.et al,FACULTY OF MEDICINE UNIVERSITY OF MALAYA KUALA LUMPUR,2017
Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress Merve Baysa.et al,PLOS ONE,2017
Alteration in follistatin gene expression detected in prenatally androgenized rats Marziyeh Salehi Jahromi.et al,Gynecol Endocrinol,2017
Cynanchum wilfordii Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia by Regulating 5a-Reductase and Androgen Receptor Activities in a Rat Model Lee G.et al,Nutrients,2017
The Effects of Testosterone Deficiency and Its Replacement on InflammatoryMarkers in Rats: A Pilot Study Kok-Yong Chin.et al,Endocrinology and Metabolism,2017
Citalopram Induces Reproductive Toxicity in Male Rats Ilgin S.et al,Birth Defects Res,2017
Effect of amifostine on sperm DNA fragmentation and testes after radioiodine treatment Cigdem Cebi Sen.et al,Journal of Veterinary Research,2017
Role of early environmental enrichment on the social dominance tube test at adulthood in the rat Cao WY.et al,Psychopharmacology (Berl).,2017
Effect of Intravenous Administration of Cobalt Chloride to Horses on Clinical and Hemodynamic Variables Burns TA.et al,J Vet Intern Med,2017
Sertraline-induced reproductive toxicity in male rats: evaluation of possible underlying mechanisms Ozlem Atli.et al,ajandrology,2016
Preventive effects of B-cryptoxanthin against cadmium-induced oxidative stress in the rat testis Liu XR.et al,Asian J Androl.,2016
Active immunization with GnRH-tandem-dimer peptide in young male rats reduces serum reproductive hormone concentrations, testicular development and spermatogenesis Han X F.et al,Asian journal of andrology,,2016
Malfunction of Sertoli cell secretion leads to testicular damage in diabetic rats Baoxing Liu.et al,Int J Clin Exp Med,2016
Protective effects of quercetin against arsenic-induced testicular damage in rats Baltaci BB.et al,Andrologia.,2016
Age and sex dependent effects of early overnutrition on metabolic parameters and the role of neonatal androgens Argente-Arizn P.et al,Biol Sex Differ.,2016
The effects of streptozotocin-induced diabetes on ghrelin expression in rat testis: biochemical and immunohistochemical study Snmez MF et al,Folia Histochem Cytobiol,2015
High doses of caffeine reduce in vivo osteogenic activity in prepubertal rats Shin J. et al,J Anat.,2015
Honey and Vitamin E Restore the Plasma Level of Gonadal Hormones and Improve the Fertilization Capacity in Noise-Stressed Rats Rajabzadeh A.et al,A. General Policy,2015
Blockage of the neonatal leptin surge affects the gene expression of growth factors, glial proteins and neuropeptides involved in the control of metabolism and reproduction in peri-pubertal male and female rats. Mela V et al,Endocrinology,2015
Preventive effect of Pueraria mirifica on testosterone-induced prostatic hyperplasia in Sprague Dawley rats Masrudin SS.et al,Andrologia.,2015
Antimullerian hormone and its receptor gene expression in prenatally androgenized female rats. Daneshian Z.et al,Int J Endocrinol Metab.,2015
Exposure to increased levels of estradiol during development can have long-term effects on the response to undernutrition in female rats Carrillo B et al,Nutr Neurosci,2015
A Survey of the Relationship Between Noised Pollution, Honey and Vitamin E and Plasma Level of Blood Sexual Hormones in Noise-Exposed Rats Asghar Rajabzadeh et al,Jentashapir Journal of Health Research,2015
Exposure to Perfluorooctane Sulfonate In Utero Reduces Testosterone Production in Rat Fetal Leydig Cells Zhao B et al,PLoS One,2014
A Chinese herbal formula, Wuzi Yanzong Pill (), improves spermatogenesis by modulating the secretory function of Sertoli cells Xu YP et al,Chin J Integr Med,2014
Eurycomanone, the major quassinoid in Eurycoma longifolia root extract increases spermatogenesis by inhibiting the activity of phosphodiesterase and Low BS et al,J Ethnopharmacol,2013
Evaluation of effects of repeated sevoflurane exposure on rat testicular tissue and reproductive hormones Kaya Z et al,Inhal Toxicol.,2013
Prior caloric restriction increases survival of pre-pubertal obese-and PCOS-prone rats exposed to a challenge of time-limited feeding and physical activity. Diane A et al,J Appl Physiol,2013
Alterations in gene expression and steroidogenesis in the testes of transient cerebral ischemia in male rats Zhao BH et al,Chinese Medical Journal,2012
Comparative hepatic proteome analysis between lean and obese rats fed a high-fat diet reveals the existence of gender differences Xia Wang et al,PROTEOMICS,2012
Standardized quassinoid-rich Eurycoma longifolia extract improved spermatogenesis and fertility in male rats via the hypothalamic-pituitary-gonadal axis Low BS et al,J Ethnopharmacol,2012
Neonatal exposure to benzo [a] pyreneDecreases the levels of serum testosterone and histone H3K14 acetylation of the StAR promoter in the testes of SD rats Liang J et al,Toxicology,2012
Gender differences in rat plasma proteome in response to high-fat diet Hao Liu et al,PROTEOMICS,2012
Profiling of gender-specific rat plasma proteins associated with susceptibility or resistance to diet-induced obesity /,JOURNAL OF PROTEOMICS,2012
Gender Dimorphism in Skeletal Muscle Proteome Between Lean and Diet-induced Obese Rats Oh TS et al ,Cellular Physiology and Biochemistry,2011
Gender Difference in Proteome of Brown Adipose Tissues between Male and Female Rats Exposed to a High Fat Diet Choi DK et al,Cellular Physiology and Biochemistry,2011

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