Rat Testosterone,T ELISA Kit

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Name: Rat Testosterone,T ELISA Kit
Brand: CUSABIO
SKU: CSB-E05100r

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Code	CSB-E05100r
Size	96T,5×96T,10×96T
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Target Name

Testosterone,T

Alternative Names

N/A

Abbreviation

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.13 ng/mL-25.6 ng/mL

Sensitivity

0.06 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat testosteronen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	97
	Range %	92-104
1:4	Average %	85
	Range %	81-88
1:8	Average %	89
	Range %	83-93

Recovery

The recovery of rat testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
25.6	0.238	0.236	0.237
6.4	0.435	0.431	0.433
2	0.611	0.615	0.613
0.5	0.984	0.982	0.983
0.13	1.403	1.398	1.401
0	2.001	2.003	2.002

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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