Rat Testosterone,T ELISA Kit

Rat Testosterone,T ELISA Kit

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T ELISA Kit

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Rat Testosterone,T ELISA Kit

Specification

Code

CSB-E05100r

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Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Lead Time

3-5 working days

Abbreviation

Protein Biological Process 1

Sex hormone

Target Name

Testosterone,T

Alias

N/A

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.13 ng/mL-25.6 ng/mL

Sensitivity

0.06 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat testosteronen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	97
	Range %	92-104
1:4	Average %	85
	Range %	81-88
1:8	Average %	89
	Range %	83-93

Recovery

The recovery of rat testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
25.6	0.238	0.236	0.237
6.4	0.435	0.431	0.433
2	0.611	0.615	0.613
0.5	0.984	0.982	0.983
0.13	1.403	1.398	1.401
0	2.001	2.003	2.002

References

Gender Difference in Proteome of Brown Adipose Tissues between Male and Female Rats Exposed to a High Fat Diet Choi DK et al,Cellular Physiology and Biochemistry,2011 28(5):933-48
Gender Dimorphism in Skeletal Muscle Proteome Between Lean and Diet-induced Obese Rats Oh TS et al ,Cellular Physiology and Biochemistry,2011 28(5):981-96
Profiling of gender-specific rat plasma proteins associated with susceptibility or resistance to diet-induced obesity /,JOURNAL OF PROTEOMICS,2012 Feb 2;75(4):1386-400.
Gender differences in rat plasma proteome in response to high-fat diet Hao Liu et al,PROTEOMICS,2012 Jan;12(2):269-83.
Neonatal exposure to benzo [a] pyreneDecreases the levels of serum testosterone and histone H3K14 acetylation of the StAR promoter in the testes of SD rats Liang J et al,Toxicology,2012 Dec 16;302(2-3):285-91.
Standardized quassinoid-rich Eurycoma longifolia extract improved spermatogenesis and fertility in male rats via the hypothalamic-pituitary-gonadal axis Low BS et al,J Ethnopharmacol,2013 Feb 13;145(3):706-14. d
Comparative hepatic proteome analysis between lean and obese rats fed a high-fat diet reveals the existence of gender differences Xia Wang et al,PROTEOMICS,2012 Jan;12(2):284-99.
Alterations in gene expression and steroidogenesis in the testes of transient cerebral ischemia in male rats Zhao BH et al,Chinese Medical Journal,2012 Jun;125(12):2168-72
Prior caloric restriction increases survival of pre-pubertal obese-and PCOS-prone rats exposed to a challenge of time-limited feeding and physical activity. Diane A et al,J Appl Physiol,2013 Feb 28.
Evaluation of effects of repeated sevoflurane exposure on rat testicular tissue and reproductive hormones Kaya Z et al,Inhal Toxicol.,2013 Mar;25(4):192-8.
Eurycomanone, the major quassinoid in Eurycoma longifolia root extract increases spermatogenesis by inhibiting the activity of phosphodiesterase and Low BS et al,J Ethnopharmacol,2013 Jun 26.pii: S0378-8741(13)00443-1.
A Chinese herbal formula, Wuzi Yanzong Pill (), improves spermatogenesis by modulating the secretory function of Sertoli cells Xu YP et al,Chin J Integr Med,2014 Mar;20(3):194-9.
Exposure to Perfluorooctane Sulfonate In Utero Reduces Testosterone Production in Rat Fetal Leydig Cells Zhao B et al,PLoS One,2014 Jan 14;9(1):e78888.
A Survey of the Relationship Between Noised Pollution, Honey and Vitamin E and Plasma Level of Blood Sexual Hormones in Noise-Exposed Rats Asghar Rajabzadeh et al,Jentashapir Journal of Health Research,2015 Feb;6(1): e27331
Exposure to increased levels of estradiol during development can have long-term effects on the response to undernutrition in female rats Carrillo B et al,Nutr Neurosci,2015 Mar 12.
Antimullerian hormone and its receptor gene expression in prenatally androgenized female rats. Daneshian Z.et al,Int J Endocrinol Metab.,13(1):e19511
Preventive effect of Pueraria mirifica on testosterone-induced prostatic hyperplasia in Sprague Dawley rats Masrudin SS.et al,Andrologia.,47(10):1153-9
Blockage of the neonatal leptin surge affects the gene expression of growth factors, glial proteins and neuropeptides involved in the control of metabolism and reproduction in peri-pubertal male and female rats. Mela V et al,Endocrinology,2015 Jul;156(7):2571-81.
Honey and Vitamin E Restore the Plasma Level of Gonadal Hormones and Improve the Fertilization Capacity in Noise-Stressed Rats Rajabzadeh A.et al,A. General Policy,2015 2(2):64-68
High doses of caffeine reduce in vivo osteogenic activity in prepubertal rats Shin J. et al,J Anat.,2015 Jul;227(1):10-20
The effects of streptozotocin-induced diabetes on ghrelin expression in rat testis: biochemical and immunohistochemical study Snmez MF et al,Folia Histochem Cytobiol,2015 Mar 13.
Age and sex dependent effects of early overnutrition on metabolic parameters and the role of neonatal androgens Argente-Arizn P.et al,Biol Sex Differ.,7(1): 1
Protective effects of quercetin against arsenic-induced testicular damage in rats Baltaci BB.et al,Andrologia.,2016 Mar 18.
Malfunction of Sertoli cell secretion leads to testicular damage in diabetic rats Baoxing Liu.et al,Int J Clin Exp Med,9(6):9316-9321
Active immunization with GnRH-tandem-dimer peptide in young male rats reduces serum reproductive hormone concentrations, testicular development and spermatogenesis Han X F.et al,Asian journal of andrology,,18(3): 485.
Preventive effects of B-cryptoxanthin against cadmium-induced oxidative stress in the rat testis Liu XR.et al,Asian J Androl.,/
Sertraline-induced reproductive toxicity in male rats: evaluation of possible underlying mechanisms Ozlem Atli.et al,ajandrology,Dec 13
Effect of Intravenous Administration of Cobalt Chloride to Horses on Clinical and Hemodynamic Variables Burns TA.et al,J Vet Intern Med,Dec 29
Role of early environmental enrichment on the social dominance tube test at adulthood in the rat Cao WY.et al,Psychopharmacology (Berl).,Aug 22
Effect of amifostine on sperm DNA fragmentation and testes after radioiodine treatment Cigdem Cebi Sen.et al,Journal of Veterinary Research,Dec 27
Citalopram Induces Reproductive Toxicity in Male Rats Ilgin S.et al,Birth Defects Res,Mar 31.
The Effects of Testosterone Deficiency and Its Replacement on InflammatoryMarkers in Rats: A Pilot Study Kok-Yong Chin.et al,Endocrinology and Metabolism,Jan 9
Cynanchum wilfordii Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia by Regulating 5a-Reductase and Androgen Receptor Activities in a Rat Model Lee G.et al,Nutrients,Sep 27
Alteration in follistatin gene expression detected in prenatally androgenized rats Marziyeh Salehi Jahromi.et al,Gynecol Endocrinol,Feb 26
Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress Merve Baysa.et al,PLOS ONE,April 18
Investigating the effects of testosterone on uterine fluid regulation and endometrial receptivity in a rat model / Mohd Helmy bin Mokhtar Mohd Helmy.et al,FACULTY OF MEDICINE UNIVERSITY OF MALAYA KUALA LUMPUR,Feb 28
Lactational exposure of polychlorinated biphenyls impairs Leydig cellular steroidogenesis in F1 progeny rats Thangavelu SK.et al,Reprod Toxicol,Dec 2
Serum and Brain Metabolomic Variations Reveal Perturbation of Sleep Deprivation on Rats and Ameliorate Effect of Total Ginsenoside Treatment Xiao-jun Gou.et al,International Journal of Genomics,Aug 16
Beneficial effect of folic acid on kidney and testes of adult albino rats after exposure to methomyl Samar Sakr.et al,Toxicol. Res,Mar 15
Serum and Brain Tissue Metabolic Profile Changes on Rat Model of Sleep Deprivation and Intervention Effects of Total Ginsenoside Using Metabonomic Xiao-jun Gou1.et al,Center for Chinese Medicine Therapy and Systems Biology,/
Antioxidant activity of Spirulina platensis alleviates doxorubicin-induced oxidative stress and reprotoxicity in male rats Naglaa Z. H.et al,Oriental Pharmacy and Experimental Medicine,May 26
Protective effects of persian honey, Apis Mellifera Meda Skorikov on side effects of chemotherapy and ischemia/reperfusion induced testicular injury Mohammadreza Gholami .et al,Journal of Complementary and Integrative Medicine,May 23
TOXIC EFFECTS OF TRAZADONE ON MALE REPRODUCTIVE SYSTEM VIA DISRUPTING HYPOTHALAMIC-PITUITARY-TESTICULAR AXIS AND INDUCING TESTICULAR OXIDATIVE STRESS Sinem Ilgin.et al,/,/
Amelioration of diabetes‐induced testicular and sperm damage in rats by cerium oxide nanoparticle treatment Tayebe Artimani.et al,ANDROLOGIA,July 18
Venlafaxine and carvedilol ameliorate testicular impairment and disrupted spermatogenesis in rheumatoid arthritis by targeting AMPK/ERK and PI3K/AKT/mTOR pathways Eid AH, et al,Toxicology and applied pharmacology,December 19
Venlafaxine and carvedilol ameliorate testicular impairment and disrupted spermatogenesis in rheumatoid arthritis by targeting AMPK/ERK and PI3K/AKT/mTOR pathways Eid AH, et al,Toxicology and applied pharmacology,December 19
Impact of Sleep Deprivation on the Hypothalamic-Pituitary-Gonadal Axis and Erectile Tissue Lee DS, et al,Journal of Sexual Medicine,January 7
Prenatal exposure to testosterone induces cardiac hypertrophy in adult female rats through enhanced Pkcδ expression in cardiac myocytes Hou M, et al,Journal of Molecular and Cellular Cardiology,January 11

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Check Code：

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.