Rat Thioredoxin-interacting protein(TXNIP) ELISA kit

Instructions
Code CSB-EL025383RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name thioredoxin interacting protein
Alternative Names Txnip ELISA Kit; Vdup1 ELISA Kit; Thioredoxin-interacting protein ELISA Kit; Vitamin D3 up-regulated protein 1 ELISA Kit
Abbreviation TXNIP
Uniprot No. Q5M7W1
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TXNIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 86-95
1:2 Average % 102
Range % 97-107
1:4 Average % 91
Range % 85-97
1:8 Average % 97
Range % 91-103
Recovery
The recovery of rat TXNIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 85-96
EDTA plasma (n=4) 92 87-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.507 2.536 2.522 2.348
1500 1.964 1.834 1.899 1.725
750 1.278 1.304 1.291 1.117
375 0.735 0.730 0.733 0.559
187.5 0.482 0.492 0.487 0.313
94 0.364 0.372 0.368 0.194
47 0.267 0.261 0.264 0.090
0 0.173 0.175 0.174  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth (By similarity).
Gene References into Functions
  1. This study found that acupuncture treatment alleviated cognitive decline and hippocampal neuronal death by inhibiting cerebral oxidative stress and inflammation, which was probably through downregulating TXNIP expression. PMID: 29110407
  2. TXNIP contributes to the dysregulation of tubular autophagy and mitophagy in diabetic nephropathy through activation of the mTOR signaling pathway. PMID: 27381856
  3. results demonstrated, for the first time, that SalA protects against HFD-induced NAFLD by ameliorating hepatic lipid accumulation and inflammation, and these protective effects may partially due to regulation of the TXNIP/NLRP3 and TXNIP/ChREBP pathways. PMID: 27345365
  4. The results of this study may represent the critical evidence to support the pro-inflammatory and pro-apoptotic effects of TXNIP after SAH. PMID: 28490373
  5. TXNIP level is also significantly upregulated in the diabetic rat retina in vivo and induces radial glial fibrillary acidic protein expression, a marker for Muller glia activation, and the formation of LC3BII puncta, which are prevented by intravitreal injection of TXNIP siRNA. PMID: 28492550
  6. histone acetylation serves as a key regulator of glucose-induced increase in TXNIP gene expression PMID: 27989964
  7. TXNIP may participate in EBI after SAH by mediating apoptosis. The blockage of TXNIP induced by PERK could be a potential therapeutic strategy for SAH treatment. PMID: 28420192
  8. Down-regulation of TXNIP or inhibition of NLRP3 contributed to the suppression of NLRP3 inflammasome activation, inflammation, and re-balanced lipid metabolism. In contrast, overexpression of TXNIP or agonism of NLRP3 exacerbated the cellular damage induced by sodium palmitate. PMID: 26133299
  9. high glucose and lipopolysaccharide activate ROS/TXNIP/ NLRP3/IL-1beta inflammasome signaling in glomerular mesangial cells PMID: 26881256
  10. ITCH targets TXNIP for ubiquitin-proteasome degradation in cardiomyocytes and ameliorates reactive oxygen species-induced cardiotoxicity through the thioredoxin system. PMID: 26796253
  11. These findings thereby provide new mechanistic insight into the regulation of TXNIP and beta-cell biology and reveal novel links between proinflammatory cytokines, carbohydrate response element binding protein-mediated transcription, and microRNA signaling. PMID: 26858253
  12. characterized TXNIP as a presently unrecognized molecule implicated in the regulatory actions of Cx43 on oxidative drug injury. PMID: 26154105
  13. Androgen receptor silences thioredoxin-interacting protein and competitively inhibits glucocorticoid receptor-mediated apoptosis in pancreatic beta-Cells.( PMID: 25639671
  14. A novel mechanism is provided for fructose-induced nonalcoholic fatty liver disease pathogenesis by which the ROS-TXNIP pathway mediates hepatocellular NLRP3 inflammasome activation, inflammation and lipid accumulation. PMID: 25602171
  15. Evidence for enhanced TXNIP expression in hypertension and HFD-induced retinal oxidative/inflammatory response and suggest that TXNIP is required for HFD-mediated activation of the NLRP3 inflammasome and the release of IL-1beta in endothelial cells PMID: 24201577
  16. Data suggest hepatic expression of Txnip can be regulated by dietary factors; here, quercetin, an antioxidant dietary supplement, down-regulates expression of Txnip in liver of rats with type 1 diabetes to prevent non-alcoholic fatty liver disease. PMID: 23647015
  17. Hyperglycemia enhances myocardial Txnip expression, possibly through reciprocally modulating p38 MAPK and Akt activation, leading to aggravated oxidative stress and amplification of cardiac injury following myocardial ischemia-reperfusion injury. PMID: 23305039
  18. TXNIP up-regulation under chronic hyperglycemia is critically involved in cellular oxidative stress, DNA damage and retinal pericyte apoptosis. PMID: 23353834
  19. hyperglycemia sustains TXNIP up-regulation in Muller glia and evokes a program of cellular defense/survival mechanisms that ultimately lead to oxidative stress, ER stress/inflammation, autophagy and apoptosis. PMID: 22474421
  20. Enhanced TXNIP expression disrupted Trx/ASK-1 inhibitory complex leading to release of ASK-1 and activation of the pro-apoptotic p38 MAPK/JNK pathway, as indicated by cleaved PARP and caspase-3 expression. PMID: 21434880
  21. TXNIP has a critical role in inflammation and retinal injury in early stages of diabetic retinopathy. PMID: 21364670
  22. These results suggest that high glucose exposure improves Txnip mRNA and protein expression level by reactive oxygen species/MEK/MAPK signaling pathway. PMID: 20953987
  23. Data show that TXNIP shuttles between subcellular compartments in response to oxidative stress and identified a novel redox-sensitive mitochondrial TXNIP-Trx2-ASK1 signaling cascade. PMID: 19959470
  24. Fatty acids do not induce beta-cell expression of proapoptotic TXNIP, but TXNIP deficiency specifically inhibits the mitochondrial death pathway underlying beta-cell glucotoxicity. PMID: 19875615
  25. Study shows evolutionarily conserved expression patterns of VDUP1 in Drosophila and rat nervous systems, including subcellular localization--cytoplasmic enrichment in neurons and nuclear expression in glia. PMID: 19824090
  26. The thioredoxin inhibitor Txnip is a critical regulator of biomechanical signaling. PMID: 15123525
  27. results indicate a role for Txnip in the metabolic response to feeding and the maintenance of the redox status PMID: 15520447
  28. results suggest that loss of thioredoxin-binding protein-2 is essential for proliferation of neoplastic and non-neoplastic renal tubular cells & that it is a target gene in oxidative stress-induced renal carcinogenesis by ferric nitrilotriacetate [TBP-2] PMID: 15834431
  29. direct role for VDUP1 in the adverse effects of ischemia and oxidative stress on cardiomyocyte survival, left ventricular collagen deposition, and cardiac function PMID: 16172122
  30. individual genes and groups of genes were found to be dysregulated in diabetic neuropathy; the most significant of these was thioredoxin interacting protein (Txnip PMID: 16938273
  31. soleus muscle subjected to hindlimb unloading resulted in an increase in mRNA expression of TBP-2, a negative regulator of thioredoxin, followed by muscle atrophy PMID: 16983998
  32. Results suggest that high glucose induces Txnip through a TGF-beta1-independent pathway. PMID: 17675577
  33. The high olive oil diet does not change the tumour expression of H19 and VDUP1. PMID: 19337063
  34. TXNIP over-expression in endothelial cells abolishes H3K9 tri-methylation, a marker for gene inactivation, and increases H3K9 acetylation, an indicator of gene induction, at proximal Cox2 promoter bearing the NF-kappaB-binding site. PMID: 19562690

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Subcellular Location Cytoplasm
Protein Families Arrestin family
Database Links

KEGG: rno:117514

STRING: 10116.ENSRNOP00000028793

UniGene: Rn.2758

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