Code | CSB-E13102r |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat TH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 90 | ||||
Range % | 85-96 | |||||
1:200 | Average % | 96 | ||||
Range % | 91-105 | |||||
1:400 | Average % | 98 | ||||
Range % | 92-104 | |||||
1:800 | Average % | 94 | ||||
Range % | 86-98 |
The recovery of rat TH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 90 | 85-94 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Rat tyrosine hydroxylase (TH) ELISA Kit was designed for the quantitative measurement of Rat tyrosine hydroxylase (TH) protein in serum, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL .
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I would like to know if fixed 4% PFA tissue will work in this kit?
I would like to know if can use another lysis buffer with the PBS for homogenization? So he can protect the protein from degradation. Or should he just stick to using PBS only?
I am planning to run trail run with the plate and is not using the whole plate wells.
Is it possible if he had it in separate columns to run it?
Are there two vials of standard provided in the kit, so they can assay twice? And the stock solution of the standard can be stored at 2-8℃ for 5 days?
Thank you for your help and patience.I have another question about the kit. As he states in his own words:
“I just got the last question for you, the adhesive strip, If we are using the portion of the wells, can this be cut and be used and saved for later use as well? since there is only 4 of them and I was wondering if we could do that and if not, can we use a different adhesive strip, if this adhesive strip is only to protect the well from residues or the contamination issue?”
Please provide the following information regarding Rat Tyrosine Hydroxylase, TH ELISA Kit (CSB-E13102r)
a. Is there a predefined protocol on the SpectraMax M3 plate reader for this assay for wavelength correction?
b. Customer would like to know if the lab recommends 540 or 570 nm for the wavelength correction?
c. Also, the plate contains 12 strips of 8 wells, correct?
d. Also, can you please clarify if the kit is good for only one month at 2-8 degC? Or can it be stored longer?
Thank you for your response.I also would like to know if the lab recommends path length check on the plate reader?