Rat Vitamin D3 receptor(VDR) ELISA kit

Instructions
Code CSB-EL025832RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name vitamin D (1,25- dihydroxyvitamin D3) receptor
Alternative Names Vdr ELISA kit; Nr1i1Vitamin D3 receptor ELISA kit; VDR ELISA kit; 1,25-dihydroxyvitamin D3 receptor ELISA kit; Nuclear receptor subfamily 1 group I member 1 ELISA kit
Abbreviation VDR
Uniprot No. P13053
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.2 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat VDR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 84-93
1:2 Average % 101
Range % 98-105
1:4 Average % 96
Range % 93-99
1:8 Average % 105
Range % 101-109
Recovery
The recovery of rat VDR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 84 81-89
EDTA plasma (n=4) 95 91-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 1.927 1.958 1.943 1.859
1000 1.537 1.568 1.553 1.469
500 1.176 1.131 1.154 1.070
250 0.789 0.742 0.766 0.682
125 0.417 0.401 0.409 0.325
62.5 0.253 0.264 0.259 0.175
31.2 0.134 0.138 0.136 0.052
0 0.087 0.081 0.084  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Recruited to promoters via its interaction with BAZ1B/WSTF which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
Gene References into Functions
  1. Vitamin D inhibits lymphangiogenesis through VDR-dependent mechanisms. PMID: 28303937
  2. VDR and PDIA3/1,25MARRS genes were silenced separately or simultaneously in E16 primary rat cortical neurons PMID: 28707894
  3. it is conceivable that the activation of the ERK1/2 pathway induced by VDR activation may have an essential role in the evasion of apoptosis after global cerebral ischemia . PMID: 29138801
  4. These results demonstrated the localization of VDR on the neuronal plasma membrane and the co-localization of VDR and APP or ADAM10 or Nicastrin and limited co-localization of VDR and PS1. PMID: 29176823
  5. vitamin D receptor analog VS-105 and paricalcitol have an effect on chronic kidney disease-mineral bone disorder in an experimental model of chronic kidney disease PMID: 27818277
  6. Liganded vitamin D receptor through its interacting repressor inhibits the expression of Col1a1. PMID: 27351590
  7. Renal VDR expression is decreased in spontaneously hypertensive rats before the development of hypertension. PMID: 27009470
  8. Acute bout of resistance exercise increases vitamin D receptor protein expression in rat skeletal muscle. PMID: 26347486
  9. VDR may mediate the increased expression levels of BMP2, Runx2 and Osterix by positively regulating calcium levels in primary primary renal tubular epithelial cells. PMID: 25823394
  10. Antenatal endotoxin disrupts lung vitamin D receptor and 25-hydroxyvitamin D 1alpha-hydroxylase expression in the developing rat. PMID: 26342089
  11. Thus, VDR in the apical brush border of the proximal convoluted tubule cells serves to "sense" the level of circulating 1,25(OH)2D3 and modulates the activity of the 1alpha-hydroxylase and the 24-hydroxylase accordingly. PMID: 25425001
  12. VS-105 may provide cardiovascular benefits in 5/6 nephrectomized rats via VDR activation. PMID: 25503724
  13. Show VDR in the adult rodent brain using proteomic techniques; in the embryo VDR distribution is most prominent in the nucleus; by adulthood that this has reversed with a prominent membrane component for VDR in the gut and kidney but not the brain PMID: 24607320
  14. these findings suggest that functional cooperation between Vdr and Runx2 is necessary for vascular calcification in response to vitamin D3. PMID: 24349534
  15. A significant increase in VDR expression was observed in DRG neurons of diabetic rats. PMID: 23684983
  16. crystal structures of the ligand-binding domain of rat VDR (VDR-LBD) in ternary complexes with a synthetic partial peptide of the coactivator MED1 (mediator of RNA polymerase II transcription subunit 1) and four ligands PMID: 23723390
  17. Calcitriol promotes vascular calcification through systemic vitamin D receptors rather than vascular receptors. PMID: 24202304
  18. we confirm that the VDR is present in the nucleus of tyrosine hydroxylase (TH)-positive neurons in both the human and rat substantia nigra PMID: 23352937
  19. Data indicate that ligands changed the conformation of the vitamin D receptor (VDR), resulting in different hydrogen-bond networks depending on the potency of the ligand. PMID: 23462137
  20. There is a role for VDR in increasing the brain clearance of P-gp substrates, including human amyloid Abeta42 protein, a plaque-forming precursor in Alzheimer's disease. PMID: 23035695
  21. In studies of conformational changes in ligand-binding domain of VDR, NMR data suggest that ligand-specific chemical shifts map not only to residues at/near binding pocket but also to residues remote from ligand-binding site. PMID: 22112050
  22. Over-expression of VDR in cellular plasma and nuclear membranes of osteoblasts was found in bone of immature rats with disuse osteoporosis. PMID: 20369481
  23. Nuclear vitamin D receptor immunoreactivity was present within nearly all neurons, while cytoplasmic VDR was found preferentially in unmyelinated calcitonin gene-related peptide (CGRP)-positive neurons, colocalizing with CYP27B1 and CYP24. PMID: 20969950
  24. analysis of the effect of the vitamin D analog, paricalcitol, a VDR activator (VDRA), on the progression of cardiomyopathy in the 5/6 nephrectomized uremic model PMID: 20236614
  25. association between VDR and caveolin-3 and the regulation of this interaction by 1,25(OH)2D3 are fundamentally important in understanding 1,25(OH)2D3 signal transduction in heart cells PMID: 20304057
  26. Hr and VDR interact via multiple protein-protein interfaces, catalyzing histone demethylation to effect chromatin remodeling and repress the transcription of VDR target genes that control the hair cycle. PMID: 20512927
  27. Enhancement of VDR-mediated transcription by phosphorylation: correlation with increased interaction between the VDR and DRIP205, a subunit of the VDR-interacting protein coactivator complex PMID: 11818502
  28. Tissue-specific down-regulation of VDR by hypocalcemia blocks the 1,25-(OH)(2)D(3) suppression of the 1alpha-OHase and upregulation of the 24-OHase in the kidney, causing a marked accumulation of 1,25-(OH)(2)D(3) in the plasma. PMID: 12054486
  29. VDR protein localizes in the nuclei of cardiac muscle fibres; VDR mRNA expression changes over different periods of development from embryo to adult PMID: 12107506
  30. ICER has a key regulatory role in the PKA enhancement of VDR transcription and therefore in the cross-talk between the PKA signaling pathway and the vitamin D endocrine system PMID: 12198242
  31. the effect of estrogen and progesterone on the expression of dihydroxyvitamin D3 receptor (VDR) mRNA in the liver of ovariectomized rats PMID: 12297474
  32. Extracellular Ca regulates vitamin D receptor expression by parathyroid cells independently of calcitriol and by this mechanism hypocalcemia may prevent the feedback of calcitriol on the parathyroids. PMID: 12444213
  33. rat hepatocytes express very low VDR(n) messenger RNA (mRNA) and protein levels. PMID: 12717384
  34. Reduced expression of p21 and p27, being VDR dependent, is major pathogenic factor for nodular parathyroid gland growth in advanced secondary hyperparathyroidism. Review. PMID: 12771291
  35. Data show that in osteoblastic cells, the vitamin D receptor interacts directly with Runx2, and that this interaction contributes significantly to vitamin D3-dependent enhancement of the osteocalcin promoter. PMID: 15456860
  36. In vaginal epithelium, the presence of VDR was shown. During the estrous cycle, VDR has an important role in the proliferation and differentiation of vaginal squamous epithelium that is similar to the effects of estrogen. PMID: 15589866
  37. Binding of the ligands to ligand binding domain of rat VDR results in a shift of both Trp H(epsilon1) and N(epsilon1) resonances to lower frequencies PMID: 16130132
  38. ASBT gene expression is activated by 1,25(OH)(2)D(3) by specific binding to the VDRE and that such activation enhances ileal bile acid transport. PMID: 16481392
  39. Our results suggest that vitamin D3 may play a role in mechanisms relevant to protective properties against the neurotoxicity of glutamate through upregulation of VDR expression in cultured rat cortical neurons. PMID: 16521124
  40. These findings suggest that an unknown serum factor modulates the transactivation function of the vitamin D receptor. PMID: 16835013
  41. Expression of the classic vitamin D3 receptor in osteoblasts is required to generate a rapid 1alpha,25-dihydroxy vitamin D3-mediated increase in the intracellular Ca(2+) concentration. PMID: 16927375
  42. We also confirmed the ability of VDR to repress LXRalpha transcriptional activation using a synthetic LXRalpha responsive reporter. PMID: 17054913
  43. Depleted uranium affects both vitamin D active form and receptor expression and consequently could modulate the expression of cyp24a1 and vitamin D target genes. PMID: 17118558
  44. Lithocholic acid can induce its own catabolism through the Vdr. PMID: 17535892
  45. In the presence of a full-length side chain, the 20S configuration improves binding of specific proteins to the VDR transcriptional complex while modifications at carbon 2 do not. PMID: 17658451
  46. These results support a model where VDR preferentially recruits SRC-1 to enhance bone-specific OC gene transcription. PMID: 17786964
  47. 1,25(OH)(2)D(3) signal transduction in heart cells provides further evidence that the VDR plays a role in heart structure and function PMID: 17974622
  48. Intestinal vitamin D receptor protein levels and 1alpha,25(OH)(2)D(3) binding were diminished with ageing. PMID: 18060514
  49. A chronic exposure to enriched uranium affects both mRNA and protein expressions of renal nuclear receptors involved in vitamin D metabolism, without any modification of the circulating vitamin D. PMID: 18502116
  50. These data suggest the vitamin D receptor may play a role in the pilocarpine-induced epilepsy. PMID: 18534255

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Subcellular Location Nucleus, Cytoplasm
Protein Families Nuclear hormone receptor family, NR1 subfamily
Tissue Specificity Detected in intestine and kidney.
Database Links

KEGG: rno:24873

STRING: 10116.ENSRNOP00000011601

UniGene: Rn.10911

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