Rat adiponectin,ADP ELISA Kit

Code CSB-E07271r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q8K3R4
Lead Time 3-5 working days
Abbreviation ADP
Protein Biological Process 1 Cardiovascular
Target Name adiponectin,ADP
Alias N/A
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

 

Linearity

 

To assess the linearity of the assay, samples were spiked with hig3h concentrations of rat ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

 

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

References
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Q&A and Customer Reviews

Customer Reviews
  • Sample type: Serum
    Sample species: Rat
    Review: The dietary fructose treatment was associated with a significant lower concentration of adiponectin and higher concentration of triglycerides than the starch treatment.
    PMID: 29617310
  • Sample type: Plasma
    Sample species: Rat
    Review: The concentrations of adiponectin in the plasma were determined using validated rat enzyme immunoassay kits. Antioxidant status and markers of glucose and lipid metabolism in the plasma of the rats fed experimental diets.
    PMID: 31000255
  • Sample type: Plasma
    Sample species: Rat
    Review: Plasma leptin (A) and plasma adiponectin (B) levels at the end of the study in the different groups: standard-fed rats (C), standard-fed rats receiving 1 g kg−1 day−1 of Egg White Hydrolysate (EWH) (CH), diet induced obese rats (MS) and diet induced obese rats receiving 1 g kg−1 day−1 of EWH (MSH). The rats were treated with pepsin EWH since week 10 until the end of the study (week 20). Values are means ± SEM (n = 7–10). Different letters mean that values are significantly different (p < 0.05) among groups.
    PMID: 29114652
  • Sample type: Plasma
    Sample species: Rat
    Review: Measurement of plasma level of adiponectin, irisin, and PI3K: Adiponectin, irisin and PI3K activity were measured using the commercial ELIZA kits following the manufacturer's instructions.
    PMID: 30684496

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