Rat alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit

Code CSB-EL001654RA
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q64240
Lead Time 7-14 working days
Abbreviation AMBP
Protein Biological Process 1 Tumor marker
Target Name alpha-1-microglobulin/bikunin precursor
Alias A1M, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI, alpha-1-microglobulin/bikunin|bikunin|complex-forming glycoprotein heterogeneous in charge|growth-inhibiting protein 19|inter-alpha-trypsin inhibi
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.563 ng/mL-100 ng/mL
Sensitivity 0.649 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 105
Range % 99-109
1:200 Average % 96
Range % 92-99
1:400 Average % 95
Range % 90-98
1:800 Average % 89
Range % 84-94
Recovery
The recovery of rat AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 87-95
EDTA plasma (n=4) 99 99-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
100 0.116 0.124 0.120
50 0.231 0.245 0.238
25 0.478 0.498 0.488
12.5 1.019 1.025 1.022
6.25 1.426 1.367 1.397
3.125 2.159 2.168 2.164
1.563 2.466 2.544 2.505
0 2.845 2.654 2.750
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Function Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization (By similarity).
Subcellular Location Secreted
Protein Families Calycin superfamily, Lipocalin family
Tissue Specificity Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine. Trypstatin is present in mast cell gra
Database Links

KEGG: rno:25377

STRING: 10116.ENSRNOP00000009248

UniGene: Rn.18721

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