Rat alpha-1-microglobulin/bikunin precursor (AMBP) ELISA kit

Code CSB-EL001654RA
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
alpha-1-microglobulin/bikunin precursor
Alternative Names
Ambp ELISA Kit; ItilProtein AMBP [Cleaved into: Alpha-1-microglobulin; Inter-alpha-trypsin inhibitor light chain ELISA Kit; ITI-LC ELISA Kit; Bikunin ELISA Kit; HI-30); Trypstatin] ELISA Kit
Abbreviation
AMBP
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.563 ng/mL-100 ng/mL
Sensitivity
0.649 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 105
Range % 99-109
1:200 Average % 96
Range % 92-99
1:400 Average % 95
Range % 90-98
1:800 Average % 89
Range % 84-94
Recovery
The recovery of rat AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 87-95
EDTA plasma (n=4) 99 99-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
100 0.116 0.124 0.120
50 0.231 0.245 0.238
25 0.478 0.498 0.488
12.5 1.019 1.025 1.022
6.25 1.426 1.367 1.397
3.125 2.159 2.168 2.164
1.563 2.466 2.544 2.505
0 2.845 2.654 2.750
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat AMBP ELISA Kit was designed for the quantitative measurement of Rat AMBP protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.563 ng/mL-100 ng/mL and the sensitivity is 0.649 ng/mL.

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Target Background

Function
(From Uniprot)
Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.; Trypstatin is a trypsin inhibitor. It inhibits blood coagulation factor Xa and tryptase about 100-fold more rapidly than porcine pancreatic trypsin and chymase.
Subcellular Location
Secreted.
Protein Families
Calycin superfamily, Lipocalin family
Tissue Specificity
Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine. Trypstatin is present in mast cell gra
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