Rat bone alkaline phosphatase,BALP ELISA Kit

Instructions
Code CSB-E11865r
Size 96T,5×96T,10×96T

Product Details

Target Name bone alkaline phosphatase,BALP
Alternative Names Alkaline phosphatase, tissue-nonspecific isozyme (AP-TNAP) (TNSALP) (EC 3.1.3.1) (Alkaline phosphatase liver/bone/kidney isozyme), Alpl
Abbreviation BALP
Uniprot No. P08289
Species Rattus norvegicus (Rat)
Sample Types serum, tissue homogenates, plasma
Detection Range 15.6 U/L-1000 U/L
Sensitivity 3.9 U/L
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %83-96
1:2Average %96
Range %92-105
1:4Average %99
Range %96-105
1:8Average %95
Range %90-104
Recovery
The recovery of rat BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9388-99
EDTA plasma (n=4)9592-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/LOD1OD2AverageCorrected
10002.561 2.375 2.468 2.301
5001.854 1.886 1.870 1.703
2501.272 1.234 1.253 1.086
1250.805 0.817 0.811 0.644
62.50.524 0.554 0.539 0.372
31.20.362 0.353 0.358 0.191
15.60.263 0.258 0.261 0.094
00.170 0.163 0.167  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Target Data

Function This isozyme may play a role in skeletal mineralization.
Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Protein Families Alkaline phosphatase family
Database Links

KEGG: rno:25586

STRING: 10116.ENSRNOP00000019004

UniGene: Rn.82764

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.