Rat bone alkaline phosphatase,BALP ELISA Kit

Instructions
Code CSB-E11865r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name bone alkaline phosphatase,BALP
Alternative Names Alpl ELISA Kit; Alkaline phosphatase ELISA Kit; tissue-nonspecific isozyme ELISA Kit; AP-TNAP ELISA Kit; TNSALP ELISA Kit; EC 3.1.3.1 ELISA Kit; Alkaline phosphatase liver/bone/kidney isozyme ELISA Kit
Abbreviation BALP
Uniprot No. P08289
Species Rattus norvegicus (Rat)
Sample Types serum, tissue homogenates, plasma
Detection Range 15.6 U/L-1000 U/L
Sensitivity 3.9 U/L
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %83-96
1:2Average %96
Range %92-105
1:4Average %99
Range %96-105
1:8Average %95
Range %90-104
Recovery
The recovery of rat BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9388-99
EDTA plasma (n=4)9592-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
U/LOD1OD2AverageCorrected
10002.561 2.375 2.468 2.301
5001.854 1.886 1.870 1.703
2501.272 1.234 1.253 1.086
1250.805 0.817 0.811 0.644
62.50.524 0.554 0.539 0.372
31.20.362 0.353 0.358 0.191
15.60.263 0.258 0.261 0.094
00.170 0.163 0.167  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Data

Function This isozyme may play a role in skeletal mineralization.
Gene References into Functions
  1. he rats body weight were decreased, the cartilage histological structure were disrupted, the cartilage and serum contents of Al and the serum level of C-telopeptide of type II collagen were all increased, the serum level of type II collagen (Col II) and alkaline phosphatase (ALP), and the mRNA expressions of TGF-beta1, BMP-2, ALP and Col II were all decreased in the AlCl3-treated groups compared with those in control g... PMID: 27885436
  2. Data show that nonylphenol (NP) increased serum alkaline phosphatase, lead to liver damage and can increase the expression of heme oxygenase-1 (HO1) and growth arrest and DNA-damage-inducible 45 beta protein (Gadd45b) genes and may modify the toxic effects on liver through induction of oxidative stress. PMID: 27590577
  3. TNAP contributes to the hypertrophic effect of phenylephrine, as well as its ability to produce cardiomyocyte calcification. These responses are minimized by CD73-dependent endogenously produced adenosine. PMID: 24894822
  4. TNAP histochemistry offers a more straightforward, but also more sensitive, method for investigating retinal strata and their diabetes-induced degeneration. PMID: 25411053
  5. Rat pups fed IAP had decreased mRNA expression of the inflammatory cytokines TNFalpha, IL-6 and iNOS. PMID: 24888842
  6. BMP-2 and ALP gene expression is induced by a BMP-2 gene-fibronectin-apatite composite layer PMID: 21636885
  7. The expression of ENPP1, TNAP and ANK proteins could be detected in each generation of endplate chondrocytes. With the continuing passage of cells, the level of protein expression declined gradually. PMID: 21418901
  8. Data show that alkaline phosphatase (AP) activity in brain vessels and parenchyma in which AP exhibits specific patterns is attributable to TNAP. PMID: 21191615
  9. Forty SNPs in ALPL and 14 SNPs in ENPP1 genetic loci as well as pairwise haplotypes were tested for association with bone strength related traits. PMID: 19931660
  10. Characterization of rat Alpl isoforms by hplc and agar gel electrophoresis are reported. PMID: 19084045
  11. ATP-mediated DBS, forming a negative feedback loop. The duodenal epithelial brush border IAP-P2Y-HCO(3-) surface microclimate pH regulatory system effectively protects the mucosa from acid injury. PMID: 19451200

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Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Protein Families Alkaline phosphatase family
Database Links

KEGG: rno:25586

STRING: 10116.ENSRNOP00000019004

UniGene: Rn.82764

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