Rat bone alkaline phosphatase,BALP ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

CloseMessage

Your Good Partner in Biology Research

Tel : 301-363-4651

CLDN18.2｜CD20｜Proteinase K｜EGFR｜PD-L1｜CD276｜ACE2｜TMPRSS2｜Exosome Isolation Kits

SARS-CoV-2: Nucleoprotein｜Spike protein｜S (RBD) protein｜N Antibody｜S Antibody｜Neutralizing Ab ELISA Kit

Home
Kit
ELISA Kit
Rat bone alkaline phosphatase,BALP ELISA Kit

Name: Rat bone alkaline phosphatase,BALP ELISA Kit
Brand: CUSABIO
SKU: CSB-E11865r

^Instructions

Code	CSB-E11865r
Size	96T,5×96T,10×96T
Trial Size
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Citations
Related Products
Customer Reviews and Q&A
Target Background

Target Name

bone alkaline phosphatase,BALP

Alternative Names

Alpl ELISA Kit; Alkaline phosphatase ELISA Kit; tissue-nonspecific isozyme ELISA Kit; AP-TNAP ELISA Kit; TNSALP ELISA Kit; EC 3.1.3.1 ELISA Kit; Alkaline phosphatase liver/bone/kidney isozyme ELISA Kit

Abbreviation

BALP

Uniprot No.

P08289

Species

Rattus norvegicus (Rat)

Sample Types

serum, tissue homogenates, plasma

Detection Range

15.6 U/L-1000 U/L

Sensitivity

3.9 U/L

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-96
1:2	Average %	96
	Range %	92-105
1:4	Average %	99
	Range %	96-105
1:8	Average %	95
	Range %	90-104

Recovery

The recovery of rat BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-99
EDTA plasma (n=4)	95	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/L	OD1	OD2	Average	Corrected
1000	2.561	2.375	2.468	2.301
500	1.854	1.886	1.870	1.703
250	1.272	1.234	1.253	1.086
125	0.805	0.817	0.811	0.644
62.5	0.524	0.554	0.539	0.372
31.2	0.362	0.353	0.358	0.191
15.6	0.263	0.258	0.261	0.094
0	0.170	0.163	0.167

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Irisin Has a Protective Role against Osteoporosis in Ovariectomized Rats EN Morgan,BioMed Research International,2021
The Antioxidant tempol delays the MIA-induced osteoarthritis progression in rats via modulation of signaling pathways involving TGF-β1/SMAD3/NOX4 axis HB Abo-zala,ResearchSquare,2021
Ameliorating effect of leukotriene receptor antagonist in multi-organ toxicity induced in rat offspring, a possible role for epidermal growth factor WY Abdelzaher,Immunopharmacology and Immunotoxicology,2021
QingYan formula extracts protect against postmenopausal osteoporosis in ovariectomized rat model via active ER-dependent MEK/ERK and PI3K/Akt signal pathways Yuan Zhao,Journal of Ethnopharmacology,2020
Possible osteoprotective effects of myricetin in STZ induced diabetic osteoporosis in rats Xiaozhou Ying, et al,European Journal of Pharmacology,2019
Histological and Biochemical Investigation of the Effects of Low Intensity Pulsed Ultrasoundon Orthodontic Tooth Movement Mine Gecgelen Cesur, et al,Konuralp Medical Journal,2019
Critical-Size Alveolar Defect Treatment via TGF-ß3 and BMP-2 Releasing Hybrid Constructs Garipcan A, et al,Journal Of Biomaterials Science-Polymer Edition,2019
Bone health consequence of adjuvant anastrozole in monotherapy or associated with biochanin-A in ovariectomized rat model Mohamed AA.et al,Life Sciences,2018
Effects of curcumin and melatonin on bone formation in orthopedically expanded suture in rats: A biochemical, histological and immunohistochemical study M. G. Cesur.et al,ORTHOD CRANIOFAC RES,2018
Evaluation of the effects of gastrectomy on the development of metabolic bone disease Yuki Higashi.et al,Journal of Surgical Research,2016
Advanced Oxidation Protein Products Accelerate Bone Deterioration in Aged Rats Zeng JH et al,Exp Gerontol,2013
Icariin Protects Against Glucocorticoid-Induced Osteoporosis In Vitro and Prevents Glucocorticoid-Induced Osteocyte Apoptosis In Vivo Feng R et al,Cell Biochem Biophys,2013
Effects of Chronic Stress and Alendronate Therapy on the Osseointegration of Titanium Implants Conte Neto N et al,Clin Implant Dent Relat Res.,2013
Experimental development of bisphosphonate-related osteonecrosis of the jaws in rodents. Conte Neto N et al,Int J Exp Pathol,2013
Advanced glycation end products suppress lysyl oxidase and induce bone collagen degradation in a rat model of renal osteodystrophy Aoki C et al,Lab Invest,2013
Dose-dependent effects of genistein on bone homeostasis in rats' mandibular subchondral bone /,Acta Pharmacologica Sinica,2012
Characterization of Salvia Miltiorrhiza ethanol extract as an anti-osteoporotic agent /,BMC Complementary and Alternative Medicine,2011

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Function (From Uniprot)	This isozyme may play a role in skeletal mineralization.
Gene References into Functions	he rats body weight were decreased, the cartilage histological structure were disrupted, the cartilage and serum contents of Al and the serum level of C-telopeptide of type II collagen were all increased, the serum level of type II collagen (Col II) and alkaline phosphatase (ALP), and the mRNA expressions of TGF-beta1, BMP-2, ALP and Col II were all decreased in the AlCl3-treated groups compared with those in control g... PMID: 27885436 Data show that nonylphenol (NP) increased serum alkaline phosphatase, lead to liver damage and can increase the expression of heme oxygenase-1 (HO1) and growth arrest and DNA-damage-inducible 45 beta protein (Gadd45b) genes and may modify the toxic effects on liver through induction of oxidative stress. PMID: 27590577 TNAP contributes to the hypertrophic effect of phenylephrine, as well as its ability to produce cardiomyocyte calcification. These responses are minimized by CD73-dependent endogenously produced adenosine. PMID: 24894822 TNAP histochemistry offers a more straightforward, but also more sensitive, method for investigating retinal strata and their diabetes-induced degeneration. PMID: 25411053 Rat pups fed IAP had decreased mRNA expression of the inflammatory cytokines TNFalpha, IL-6 and iNOS. PMID: 24888842 BMP-2 and ALP gene expression is induced by a BMP-2 gene-fibronectin-apatite composite layer PMID: 21636885 The expression of ENPP1, TNAP and ANK proteins could be detected in each generation of endplate chondrocytes. With the continuing passage of cells, the level of protein expression declined gradually. PMID: 21418901 Data show that alkaline phosphatase (AP) activity in brain vessels and parenchyma in which AP exhibits specific patterns is attributable to TNAP. PMID: 21191615 Forty SNPs in ALPL and 14 SNPs in ENPP1 genetic loci as well as pairwise haplotypes were tested for association with bone strength related traits. PMID: 19931660 Characterization of rat Alpl isoforms by hplc and agar gel electrophoresis are reported. PMID: 19084045 ATP-mediated DBS, forming a negative feedback loop. The duodenal epithelial brush border IAP-P2Y-HCO(3-) surface microclimate pH regulatory system effectively protects the mucosa from acid injury. PMID: 19451200 Show More Hide All
Subcellular Location	Cell membrane, Lipid-anchor, GPI-anchor
Protein Families	Alkaline phosphatase family
Database Links	KEGG: rno:25586 STRING: 10116.ENSRNOP00000019004 UniGene: Rn.82764

Promotions

  Tel: 301-363-4651
  Email: [email protected]
  Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

Feature Products
Service
Technical Resources
Research Tools
About Us
Contact Us
- CUSABIO TECHNOLOGY LLC
- Tel: 301-363-4651
- Email: [email protected]
Join Us with

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Product >> Product Name：
Product >> Code：
Product >> Size：
Name： *
Email： *
Phone：
We recommend you to leave your phone number because occasionally our emails get flagged by spam filters. We would not disclose any of your private information to any third party.
Question/Comment： *
Your Region： *
Country State：
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
Check Code：