Rat bone alkaline phosphatase,BALP ELISA Kit

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Rat bone alkaline phosphatase,BALP ELISA Kit

Name: Rat bone alkaline phosphatase,BALP ELISA Kit
Brand: CUSABIO
SKU: CSB-E11865r

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Code	CSB-E11865r
Size	96T,5×96T,10×96T
Trial Size
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Target Data

Target Name

bone alkaline phosphatase,BALP

Alternative Names

Alpl ELISA Kit; Alkaline phosphatase ELISA Kit; tissue-nonspecific isozyme ELISA Kit; AP-TNAP ELISA Kit; TNSALP ELISA Kit; EC 3.1.3.1 ELISA Kit; Alkaline phosphatase liver/bone/kidney isozyme ELISA Kit

Abbreviation

BALP

Uniprot No.

P08289

Species

Rattus norvegicus (Rat)

Sample Types

serum, tissue homogenates, plasma

Detection Range

15.6 U/L-1000 U/L

Sensitivity

3.9 U/L

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-96
1:2	Average %	96
	Range %	92-105
1:4	Average %	99
	Range %	96-105
1:8	Average %	95
	Range %	90-104

Recovery

The recovery of rat BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-99
EDTA plasma (n=4)	95	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/L	OD1	OD2	Average	Corrected
1000	2.561	2.375	2.468	2.301
500	1.854	1.886	1.870	1.703
250	1.272	1.234	1.253	1.086
125	0.805	0.817	0.811	0.644
62.5	0.524	0.554	0.539	0.372
31.2	0.362	0.353	0.358	0.191
15.6	0.263	0.258	0.261	0.094
0	0.170	0.163	0.167

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Function	This isozyme may play a role in skeletal mineralization.
Gene References into Functions	he rats body weight were decreased, the cartilage histological structure were disrupted, the cartilage and serum contents of Al and the serum level of C-telopeptide of type II collagen were all increased, the serum level of type II collagen (Col II) and alkaline phosphatase (ALP), and the mRNA expressions of TGF-beta1, BMP-2, ALP and Col II were all decreased in the AlCl3-treated groups compared with those in control g... PMID: 27885436 Data show that nonylphenol (NP) increased serum alkaline phosphatase, lead to liver damage and can increase the expression of heme oxygenase-1 (HO1) and growth arrest and DNA-damage-inducible 45 beta protein (Gadd45b) genes and may modify the toxic effects on liver through induction of oxidative stress. PMID: 27590577 TNAP contributes to the hypertrophic effect of phenylephrine, as well as its ability to produce cardiomyocyte calcification. These responses are minimized by CD73-dependent endogenously produced adenosine. PMID: 24894822 TNAP histochemistry offers a more straightforward, but also more sensitive, method for investigating retinal strata and their diabetes-induced degeneration. PMID: 25411053 Rat pups fed IAP had decreased mRNA expression of the inflammatory cytokines TNFalpha, IL-6 and iNOS. PMID: 24888842 BMP-2 and ALP gene expression is induced by a BMP-2 gene-fibronectin-apatite composite layer PMID: 21636885 The expression of ENPP1, TNAP and ANK proteins could be detected in each generation of endplate chondrocytes. With the continuing passage of cells, the level of protein expression declined gradually. PMID: 21418901 Data show that alkaline phosphatase (AP) activity in brain vessels and parenchyma in which AP exhibits specific patterns is attributable to TNAP. PMID: 21191615 Forty SNPs in ALPL and 14 SNPs in ENPP1 genetic loci as well as pairwise haplotypes were tested for association with bone strength related traits. PMID: 19931660 Characterization of rat Alpl isoforms by hplc and agar gel electrophoresis are reported. PMID: 19084045 ATP-mediated DBS, forming a negative feedback loop. The duodenal epithelial brush border IAP-P2Y-HCO(3-) surface microclimate pH regulatory system effectively protects the mucosa from acid injury. PMID: 19451200 Show More Hide All
Subcellular Location	Cell membrane, Lipid-anchor, GPI-anchor
Protein Families	Alkaline phosphatase family
Database Links	KEGG: rno:25586 STRING: 10116.ENSRNOP00000019004 UniGene: Rn.82764

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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