Rat heat shock protein 27,HSP-27 ELISA Kit

Code CSB-E09240r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
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Product Details

Target Name
heat shock 27kDa protein 1
Alternative Names
Hspb1 ELISA Kit; Hsp27 ELISA Kit; Heat shock protein beta-1 ELISA Kit; HspB1 ELISA Kit; Heat shock 27 kDa protein ELISA Kit; HSP 27 ELISA Kit
Abbreviation
HSPB1
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.78 ng/mL-50 ng/mL
Sensitivity
0.195 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat HSP-27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 93-102
1:2 Average % 105
Range % 100-113
1:4 Average % 91
Range % 85-96
1:8 Average % 94
Range % 90-98
Recovery
The recovery of rat HSP-27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 85-93
EDTA plasma (n=4) 85 80-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
50 2.361 2.293 2.327 2.126
25 1.674 1.592 1.633 1.432
12.5 1.002 1.056 1.029 0.828
6.25 0.729 0.704 0.717 0.516
3.12 0.447 0.471 0.459 0.258
1.56 0.335 0.318 0.327 0.126
0.78 0.278 0.269 0.274 0.073
0 0.196 0.205 0.201  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat HSPB1 ELISA Kit was designed for the quantitative measurement of Rat HSPB1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

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Target Background

Function
(From Uniprot)
Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.
Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle.
Protein Families
Small heat shock protein (HSP20) family
Tissue Specificity
Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris a
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