Rat low density lipoprotein,LDL ELISA Kit

Code CSB-E11704r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name low density lipoprotein,LDL
Alternative Names N/A
Abbreviation LDL
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 μg/mL-20 μg/mL
Sensitivity 0.156 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat LDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:100Average %98
Range %93-103
1:200Average %104
Range %100-108
1:400Average %87
Range %80-94
1:800Average %97
Range %94-100
The recovery of rat LDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-99
EDTA plasma (n=4)9288-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
200.079 0.076 0.078
100.148 0.158 0.153
50.223 0.240 0.232
2.50.387 0.370 0.379
1.250.636 0.657 0.647
0.6250.957 0.939 0.948
0.3121.112 1.118 1.115
01.633 1.606 1.620
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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