Code | CSB-E13476m |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 87 | ||||
Range % | 83-90 | |||||
1:200 | Average % | 85 | ||||
Range % | 81-90 | |||||
1:400 | Average % | 95 | ||||
Range % | 92-98 | |||||
1:800 | Average % | 100 | ||||
Range % | 98-104 |
The recovery of mouse LDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 93 | 90-96 | ||||
EDTA plasma (n=4) | 98 | 93-100 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Low-density lipoprotein (LDL) is a major cholesterol-carrying lipoprotein particle that transports cholesterol from the liver to peripheral tissues. LDL plays an important role in lipid metabolism and serves as a key biomarker for cardiovascular disease risk. Elevated LDL levels are associated with atherosclerosis development and increased risk of coronary heart disease. In research settings, LDL quantification is necessary for studying lipid disorders, metabolic diseases, and testing therapeutic interventions targeting cholesterol metabolism.
The Mouse low density lipoprotein,LDL ELISA Kit (CSB-E13476m) is designed for quantitative measurement of LDL in Mus musculus samples. This competitive assay works with serum, plasma, and tissue homogenates. The detection range spans 7.8 ng/mL-500 ng/mL with sensitivity of 1.95 ng/mL. The assay requires 50-100ul sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for LDL quantification in mouse studies.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research investigating lipid metabolism and dyslipidemia in mouse models. The kit allows quantification of LDL cholesterol levels in serum samples as part of comprehensive lipid profiling studies. Researchers have used this assay alongside other lipid markers to study metabolic dysfunction and cardiovascular risk factors.
• Dyslipidemia research: Measuring LDL cholesterol levels to determine the extent of lipid metabolism disorders in experimental mouse models
• Metabolic profiling: Quantification of LDL cholesterol as part of comprehensive serum lipid analysis including triglycerides and HDL cholesterol measurements
• Cardiovascular research: Measuring LDL cholesterol levels in studies examining metabolic health and potential cardiovascular risk factors
• Serum biomarker analysis: Measurement of circulating LDL cholesterol levels in conjunction with inflammatory markers and endotoxin studies
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