Mouse low density lipoprotein,LDL ELISA Kit

Code CSB-E13476m
Size 96T,5×96T,10×96T
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Product Details

Target Name
low density lipoprotein,LDL
Alternative Names
N/A
Abbreviation
LDL
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 ng/mL-500 ng/mL
Sensitivity
1.95 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 87  
Range % 83-90  
1:200 Average % 85  
Range % 81-90  
1:400 Average % 95  
Range % 92-98  
1:800 Average % 100  
Range % 98-104  
Recovery
The recovery of mouse LDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 90-96  
EDTA plasma (n=4) 98 93-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
500 0.157 0.159 0.158    
250 0.219 0.239 0.229    
125 0.334 0.315 0.325    
62.5 0.537 0.523 0.530    
31.2 0.875 0.898 0.887    
15.6 1.199 1.187 1.193    
7.8 1.558 1.578 1.568    
0 2.503 2.534 2.519    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

Low-density lipoprotein (LDL) is a major cholesterol-carrying lipoprotein particle that transports cholesterol from the liver to peripheral tissues. LDL plays an important role in lipid metabolism and serves as a key biomarker for cardiovascular disease risk. Elevated LDL levels are associated with atherosclerosis development and increased risk of coronary heart disease. In research settings, LDL quantification is necessary for studying lipid disorders, metabolic diseases, and testing therapeutic interventions targeting cholesterol metabolism.

The Mouse low density lipoprotein,LDL ELISA Kit (CSB-E13476m) is designed for quantitative measurement of LDL in Mus musculus samples. This competitive assay works with serum, plasma, and tissue homogenates. The detection range spans 7.8 ng/mL-500 ng/mL with sensitivity of 1.95 ng/mL. The assay requires 50-100ul sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for LDL quantification in mouse studies.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research investigating lipid metabolism and dyslipidemia in mouse models. The kit allows quantification of LDL cholesterol levels in serum samples as part of comprehensive lipid profiling studies. Researchers have used this assay alongside other lipid markers to study metabolic dysfunction and cardiovascular risk factors.

Dyslipidemia research: Measuring LDL cholesterol levels to determine the extent of lipid metabolism disorders in experimental mouse models
Metabolic profiling: Quantification of LDL cholesterol as part of comprehensive serum lipid analysis including triglycerides and HDL cholesterol measurements
Cardiovascular research: Measuring LDL cholesterol levels in studies examining metabolic health and potential cardiovascular risk factors
Serum biomarker analysis: Measurement of circulating LDL cholesterol levels in conjunction with inflammatory markers and endotoxin studies

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