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Rat macrophage inflammatory protein 1β,MIP-1β ELISA kit

Protocol
Code CSB-E07421r
Size 96T,5×96T,10×96T
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Product Details

Associated names
Ccl4 ELISA Kit; Mip1b ELISA Kit; Scya4C-C motif chemokine 4 ELISA Kit; Macrophage inflammatory protein 1-beta ELISA Kit; MIP-1-beta ELISA Kit; Small-inducible cytokine A4 ELISA Kit
Abbreviation
CCL4
Uniprot No.
P50230
Species
Rattus norvegicus (Rat)
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1β/CCL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

　

Sample

Serum(n=4)

1:1

Average %

93

Range %

85-98

1:2

Average %

106

Range %

99-1112

1:4

Average %

97

Range %

90-105

1:8

Average %

91

Range %

86-100

Recovery

The recovery of rat MIP-1β/CCL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

95

83-98

EDTA plasma (n=4)

86

80-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

250

3.618

3.563

3.591

3.509

125

2.708

2.694

2.701

2.620

62.5

1.434

1.492

1.463

1.381

31.25

0.681

0.700

0.691

0.609

15.6

0.345

0.342

0.344

0.262

7.8

0.210

0.221

0.216

0.134

3.9

0.134

0.141

0.137

0.056

0

0.081

0.082

0.081

　

ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Rat CCL4 ELISA Kit was designed for the quantitative measurement of Rat CCL4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.9pg/ml-250pg/ml and the sensitivity is 1.95pg/ml.

Related Products

Ccl4 Proteins

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)
Monokine with inflammatory and chemokinetic properties.
Gene References into Functions
  1. Tong Luo Jiu Nao injection inhibits MIP-1beta expression in brain microvascular endothelial cells injured by oxygen-glucose deprivation and inhibitors microglial cell proliferation. PMID: 22366434
  2. Early and persistent up-regulation of CCL4/CCR5 signaling during epileptogenesis suggests that CCL4 signaling, rather than CCL2 signaling, could have a role in the epileptogenic process. PMID: 22353418
  3. this study we present data on long term increase in CCL4 mRNA expression and immunoreactivity following epileptogenic stimulus in a rat model of temporal lobe epilepsy. PMID: 21731074
  4. During experimental allergic encephalomyelitis, a strong activation of spinal perivascular macrophages is observed accompanied by a consistently increased expression of MIP-1 alpha. PMID: 12196270
Subcellular Location
Secreted.
Protein Families
Intercrine beta (chemokine CC) family
Database Links
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